Süheyla TÜRKYILMAZ, Şebnur HAZIMOĞLU

Hindilerde Bordetella avium antikorlarının enzyme-linked immunosorbent assay, mikroaglutinasyon ve lam aglutinasyon testleri ile belirlenmesi üzerine karşılaştırmalı bir araştırma

Bu çalışmanın amaçları solunum sistemi hastalığı semptomu bulunan hindilerden B. avium’a maruz kalmış olanların serolojik olarak saptanması için lam aglutinasyon (LAT), mikroaglutinasyon (MAT) test antijenlerinin hazırlanması ve bu testlerin duyarlılıklarının ticari enzyme-linked immunosorbent assay (ELISA) ile karşılaştırılmasıdır. Çalışmanın materyalini İzmir İl’i ve çevresinde bulunan, solunum sistemi problemi gösteren 900 ticari hindiden alınan kan serum örnekleri oluşturmaktadır. Seroprevalens ELISA, LAT ve MAT için sırasıyla % 21.6, % 13.8 ve % 12.1 olarak belirlenmiştir. Sonuç olarak; B. avium’a karşı oluşan antikorların belirlenmesinde en duyarlı testin ELISA olmasına karşın; LAT ve MAT’lerinin de bu amaçla kullanılabileceği tespit edilmiştir.

A comparative study on detection of bordetella avium antibodies in turkeys by enzyme-linked immunosorbent assay, microagglutination and serum plate agglutination tests

The aims of this study were to develop a serum plate agglutination test (LAT) antigen and microagglutination (MAT) test antigen for the serologic detection of turkeys that have been exposed to B. avium with respiratory disease symptom and to compare sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with these tests. All serum samples were collected from commercial turkey enterprises in İzmir, samples from 900 turkeys with symptoms of respiratory disease constituted the material of this study. The seroprevalence was determined as 21.6 %, 13.8 %, 12.1 % for ELISA, LAT, and MAT tests, respectively. As a conclusion, although ELISA was defined as the most sensitive test for screening of B. avium antibodies with this study, LAT and MAT’s could also be used for this purpose.

PDF

___

1. Back A, Halvorson G, Rajashekara G, Nagaraja VK (1998): Development of serum plate agglutination test to detect antibodies to Ornithobacterium rhinotracheale. J Vet Diag Invest, 10, 84–86.
2. Barbour EK, Brinton MK, Torkelson SD, Johnson JB, Poss PE (1991): An enzyme-linked immunosorbent assay for detection of Bordetella avium infection in turkey flocks: sensitivity, specifity and reproducibility. Avian Dis, 35, 308–314.
3. Barnes H J, Hofstad M S (1977): Turkey coryza: an emerging disease of young poults. J Am Vet Med Ass, 1171–1174.
4. Blackall PJ, Doheny CM (1987): Isolation and characterisation of Bordetella avium and related species and an evaluation of their role in respiratory disease in poultry. Aust Vet J, 64, 235–239.
5. Burns EH, Norman JM, Hatcher MD (1993): Fimbriae and determination of host species sfecificity of Bordetella bronchicestica. J Clin Microbiol, 31, 1838–1844.
6. Conover WJ (1999): Practical Nonparametric Statistics. John Waley Series, USA, 90-181.
7. Diker K S (1998): İmmunoloji. 294–298. Medisan Serisi, Ankara, Türkiye.
8. Hafez HM, Sting R (1999): Investigation on different Ornithobacterium rhinotracheale “ORT’’ isolates. Avian Dis, 43, 1–7.
9. Hopkins BA, Skeeles JK, Houghten GE, Story JD (1988): Development of an enzyme-linked immunosorbent assay for Bordetella avium. Avian Dis, 32, 353–361.
10. Jacwood DJ, Saif YM (1980): Development and use of microagglutination test to detect antibodies to Alcaligenes faecalis in turkeys. Avian Dis, 24, 685–701
11. Lister SA, Alexander DJ (1986): Turkey rhinotracheitis. Vet Bul, 56, 637–663.
12. Neighbor NK, Skeeles JK, Beasley JN, Kreider DL (1991): Use of an enzyme-linked immunosorbest assay to measure antibody levels in turkey breeder hens, eggs and progeny following natural infection or immunization with a commercial Bordetella avium bacterin. Avian Dis, 35, 315–320.
13. Ocak F (2005): Kanatlı hayvanlarda Bordetella avium’a karşı oluşan antikorların MAT, IFAT and ELISA teknikleri ile saptanması. Erciyes Üniv Vet Fak Derg, 2, 85–89.
14. Özdamar K (2001):SPSS ile Bioistatistik. Dördüncü baskı, Kaan Kitabevi, Ankara, Türkiye.
15. Skeeles JK, Arp LH (1998): Turkey bordetellosis (coryza). 275-287, In: Disease of Poultry. BW Calnek, HJ Barnes, CW Beard, McDougald LR, Saif YMJr (Eds), 10th ed. Iowa state University Press, Ames, Iowa, USA.
16. Slavik MF, Skeeles JK, Meınecke CF, Holloway L (1981): The involvement of Alcaligenes faecalis in turkeys submitted for diagnosis as detected by bacterial isolation and microagglutination test. Avian Dis, 25, 761–763.
17. Tsai HJ, Saif YM (1991): Detection of antibodies against Bordetella avium in turkeys by avidin-biotin enhancement of the enzyme-linked immunosorbent assay and the dot-immunobinding assay. Avian Dis, 35, 810–808.
18. Türkyılmaz S, Kaya O (2005): Detection of antibodies Ornithobacterium rhinotracheale and Bordetella avium by enzyme-linked immunosorbent assay in hens and turkeys in Aydın province of Turkey. Turk J Vet Anim Sci, 29, 897–902.
19. Türkyılmaz S, Türkyılmaz K, Kaya O (2006): A comparative study on detection of B. avium antibodies in turkeys by ELISA, SPAT and AGID tests. Turk J Vet Anim Sci, 30, 165–169.