Antimicrobial activity of Thymbra spicata L. essential oil in Turkish dry fermented sausages

Antimicrobial activity of Thymbra spicata L. essential oil in Turkish dry fermented sausages

Essential oils (EO) could address the need for alternative additives for food producers to maintain safety and quality of meat products. The aim of this study was to evaluate antimicrobial activity of Thymbra spicataL. subsb. spicataEO on Escherichia coliand Salmonella Typhimurium in Turkish dry fermented sausage (sucuk). Antimicrobial activity of EO, obtained from the collected plant, has been demonstrated in vitroand in sucuk matrices against selected foodborne pathogens. In the composition of the essential oil obtained in the study, total of 47 components (99.41%) were assayed including mainly carvacrol (43.6%), ɣ-terpinene (16.69%) and p-cymen (13.97%). Thymbra spicataL. observed to have antimicrobial effect on the related pathogens in vitro however, increased amount of EO use, to be antimicrobiologically effective in sucuk, negatively affected the organoleptic properties.It is concluded that natural additives could potentially be used as an alternative to chemicals in food technology to prevent foodborne diseases and to extend the shelf life of products. Further studies are needed to evaluate the combined and synergetic effects of different EOs and other preservation methods to cope with foodborne pathogens in the food matrices.

___

  • 1.Akgül A, Özcan M, Chialva F, et al(1999): Essential oils of four turkish wild-growing labiatae herbs: Salvia cryptantha montbr. et Auch., Satureja cuneifolia Ten., Thymbra spicata L. and Thymus cilicicus Boiss. et Bal. J Essent Oil Res, 11, 209-214.
  • 2.Aligiannis N, Kalpoutzakis E, Mitaku S, et al(2001): Composition and antimicrobial activity of the essential oils of two Origanum species. J Agric Food Chem, 49, 4168-4170.
  • 3.Association of Official Analytical Chemists(2007): Official Methods of Analysis of AOAC International. Association of Official Analysis Chemists International.
  • 4.Bagamboula CF, Uyttendaele M, Debevere J(2004): Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiol, 21, 33-42.
  • 5.Baydar H, Saǧdiç O, Özkan G, et al(2004): Antibacterial activity and composition ofessential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15, 169-172.
  • 6.Burt S(2004): Essential oils: Their antibacterial properties and potential applications in foods -A review. Int J Food Microbiol, 94, 223-253.
  • 7.Busatta C, Vidal RS, Popiolski AS, et al (2008): Application of Origanum majorana L. essential oil as an antimicrobial agent in sausage. Food Microbiol, 25, 207-211.
  • 8.Büyükünal SK, Şakar FŞ, Turhan İ, et al(2016): Geleneksel Türk fermente et ürünlerinde (sucuk ve pastırma) Salmonella spp., Listeria monocytogenes, Escherichia coli O157 ve nitrat-nitrit varlığı. Kafkas Univ Vet Fak Derg,22, 233-236.
  • 9.Chouhan S, Sharma K, Guleria S(2017): Antimicrobial activity of some essential oils presentstatus and future perspectives. Medicines, 4, 58.
  • 10.Deans SG, Svoboda KP(1989): Antibacterial activity of summer savory (Satureja hortensis L) essential oil and its constituents. J Hort Sci, 64, 205-210.
  • 11.Dorman HJD, Deans SG(2000): Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol, 88, 308-316.
  • 12.Dussault D, Vu KD, Lacroix M (2014): In vitro evaluation of antimicrobial activities of various commercial essential oils, oleoresin and pure compounds against foodpathogens and application in ham. Meat Sci, 96, 514-520.
  • 13.Erkmen O, Bozkurt H(2004): Quality characteristics of retailed sucuk (Turkish dry-fermented sausage). Food Technol Biotech, 42, 63-69.
  • 14.Farag RS, Daw ZY, Hewedi FM(1989): Antimicrobial activity of some Egyptian spice essential oils. J Food Protect, 52, 665-667.
  • 15.García-Díez J, Alheiro J, Falco V, et al(2017): Chemical characterization and antimicrobial properties of herbs and spices essential oils against pathogens and spoilage bacteria associated to dry-cured meat products. J Essent Oil Res, 29, 117-125.
  • 16.Hanafi R, Sobeh M, L Ashour M, et al(2014): Chemical composition and biological activity of essential oils of cumin and coriander fruits from Egypt. Nat Prod J, 4, 63-69.
  • 17.Hyldgaard M,Mygind T, Meyer RL(2012): Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components. Front Microbiol, 3, 12.
  • 18.Karatzas AK, Kets EPW, Smid EJ, et al(2001): The combined action of carvacrol and high hydrostatic pressure on Listeria monocytogenes scott A. J Appl Microbiol, 90, 463-469.
  • 19.Kiliç T(2006): Analysis of essential oil composition of Thymbra spicata var. spicata: Antifungal, antibacterial and antimycobacterial activities. Z Naturforsch C -Section C J Biosci, 61, 324-328.
  • 20.Marino M, Bersani C, Comi G(2001): Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae. Int J Food Microbiol, 67, 187-195.
  • 21.Marković T, Chatzopoulou P, Siljegović J,et al(2011): Chemical analysis and antimicrobial activities of the essential oils of Satureja Thymbra L. and Thymbra Spicata L. and their main components. Arch Biol Sci, 63, 457-464.
  • 22.Mendoza-Yepes MJ, Sanchez-Hidalgo LE, Maertens G, et al(1997): Inhibition of Listeria monocytogenes and other bacteria by a plant essential oil (DMC) in Spanish soft cheese. J Food Safety, 17, 47-55.
  • 23.Ozel MZ, Gogus F, Lewis AC(2003): Subcritical water extraction of essential oils from Thymbra spicata. Food Chem, 82, 381-386.
  • 24.Özbey G, Kök F, Muz A(2007): Isolation of Salmonella spp. in camel sausages from retail markets in Aydın, Turkey, and polymerase chain reaction (PCR) confirmation. Turk J Vet Anim Sci, 31, 67–71.
  • 25.Palaniappan K, Holley RA(2010): Use of natural antimicrobials to increase antibiotic susceptibility of drug resistant bacteria. Int J Food Microbiol, 140, 164-168.
  • 26.Pandit VA, ShelefLA(1994): Sensitivity of Listeria monocytogenes to rosemary (Rosmarinus officinalis L.). Food Microbiol, 11, 57-63.
  • 27.Pharmacopoeia E(2005): Determination of Essential Oils in Herbal Drugs. Council of Europe. 217.
  • 28.Saǧdiç O, Özcan M(2002): Antibacterial activity of Turkish spice hydrosols. Food Control, 14, 141-143.
  • 29.Shelef LA(1984): Antimicrobial Effects ofSpices. J Food Safety, 6, 29-44.
  • 30.Smid EJ, Gorris LGM(1999): Natural Antimicrobials For Food Preservation. 285–308. In: MS Rahman. (Ed.), Handbook of Food Preservation. Marcel Dekker, New York.
  • 31.Tarladgis BG, Watts BM, Younathan MT, et al(1960): A distillation method for the quantitative determination of malonaldehyde in rancid foods. J Am Oil Chem Soc, 37, 44–48.
  • 32.Ünlü M, Vardar-Ünlü G, Vural N, et al(2009): Chemical composition, antibacterial and antifungal activity of the essential oil of Thymbra spicata L. from Turkey. Nat Prod Res, 23, 572-579. İ
  • 33.Zhang D, Hu H, Rao Q, et al(2011): Synergistic effects and physiological responses of selected bacterial ısolates from animal feed to four natural antimicrobials and two antibiotics. Foodborne Pathog Dis, 8,1055-1062.
Ankara Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • Yayın Aralığı: 4
  • Başlangıç: 1954
  • Yayıncı: Ankara Üniversitesi Veteriner Fakültesi
Sayıdaki Diğer Makaleler

Distribution of Salmonella serovars and characterization of isolates in cattle feces and environmental samples

Melih ÇAKIN, Beren BAŞARAN KAHRAMAN, Belgi DİREN SIĞIRCI, Arzu Funda BAĞCIGİL

Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells

Ece ÇERÇİ, Hatice ERDOST

Effects of probiotics on in vitro ruminal profile and population of some cellulolytic bacteria

Ali ÇALIK, Gültekin YILDIZ, Hıdır GÜMÜŞ, Özge SIZMAZ, Oğuz Berk GÜNTÜRKÜN, Serdar SIZMAZ

Effects of dried thyme (Thymus vulgarisL.) leaves on performance, some egg quality traits and immunity in laying hens

Handan ESER, Sakine YALÇIN, İlyas ONBAŞILAR, Suzan YALÇIN

Determination of mineral concentrations in stem and seed of Juncus acutus

Nurcan ÇETİNKAYA, Funda ERDEM

The effects of dietary eggshell with membrane and olive leaf extract supplementation on performance, carcass characteristics and some biochemical parameters of quails exposed to heat stress

Yasin BAYKALIR, Zeki ERİŞİR, Seda İFLAZOĞLU MUTLU, Gonca OZAN KOCAMÜFTÜOĞLU, Ülkü Gülcihan ŞİMŞEK, Mehtap ÖZÇELİK, Mehmet Ali AZMAN, Selcen ARSLAN, Mehmet EROĞLU

The effect of sodium bentonite on growth performance and some blood parameters in post-weaning Tuj breed lambs

Gültekin YILDIZ, Oğuz MERHAN, Özlem DURNA AYDIN

Determination of the levels of serum oxidative indicator, cytokine and some biochemical parameters in horses naturally infected with Theileria equi

Ufuk KÖMÜROĞLU, Uğur ÖZDEK, Bekir OĞUZ, Yeter DEĞER

Effects of different chilling procedures on honey bees (Apis mellifera) for anesthesia

Hidayet TUTUN, Bekir ÇETİNTAV, Sedat SEVİN

Phylogenetic analysis of partial transmembrane protein gene of canine coronaviruses detected in Turkey

Bahattin Taylan KOÇ, Zeynep AKKUTAY YOLDAR, Tuba Çiğdem OĞUZOĞLU