Berkay KÜÇÜK, Salih TUNCAL, Osman AYDIN, Yılmaz ÜNAL, Yusuf Murat BAĞ, Zeynep ADIYAMAN, Mehmet ŞENES, Pınar CELEPLİ, Sema HÜCÜMENOĞLU, Bülent KILIÇOĞLU, Kemal KISMET, Ertuğrul ERTAŞ

KALSİYUM DOBESİLAT'IN İNTRAABDOMİNAL ADEZYON ÖNLEYİCİ ETKİSİ: DENEYSEL ÇALIŞMA

ÖZET: AMAÇ: Kalsiyum Dobesilat'ın (Ca-Dob) postoperatif intraabdominal adezyon oluşumunu önlemedeki etkinliğini deneysel çekal abrazyon modelinde göstermeyi amaçladık. GEREÇ VE YÖNTEMLER: Çalışmada kullanılan 30 adet Wistar Albino dişi rat randomize olarak 3 gruba ayrıldı. Sham grubuna laparotomi yapıldı, çekum batın dışına alındıktan sonra herhangi bir işlem yapılmadan batına konuldu. Kontrol grubuna çekal abrazyon ve periton rezeksiyonu sonrası herhangi bir işlem yapılmadı. Ca-Dob grubuna çekal abrazyon ve periton rezeksiyonu yapıldıktan sonra batın kapatıldı, sadece bu gruba 21 gün boyunca 100 mg/kg/gün dozunda orogastrik yolla Ca-Dob verildi. Tüm ratlar postoperatif 21. gün sakrifiye edildi. Adezyon değerlendirmesi yapıldı. Periton ve barsaktan alınan doku örnekleri inflamasyon ve fibrozis derecelerini belirlemek için histopatolojik incelemeye gönderildi. Ayrıca, biyokimyasal analiz için doku ve kan örnekleri alındı. BULGULAR: Ca-Dob uygulanan sıçanlarda inflamasyon, makroskopik adezyon ve fibrozis skorlarının kontrol grubuna göre istatistiksel olarak anlamlı derecede düşük olduğu saptandı (p<0,05).Yine, Ca- Dob grubunda doku MDA, doku FOÜ ve doku TOS değerlerinin kontrol grubuna göre istatistiksel olarak anlamlı derecede düşük olduğu görüldü (p=0,001). Ayrıca sham grubundaki doku total SH değerlerinin, kontrol grubuna göre istatistiksel olarak anlamlı derecede yüksek olduğu saptandı (p<0,05). Son olarak; Ca- Dob grubunda serum MDA ve serum TOS değerlerinin, kontrol grubuna göre istatistiksel olarak anlamlı derecede düşük olduğu görüldü (p<0,05). SONUÇ: Ca-Dob’ın çekal abrazyon modelinde adezyon oluşumunu engellemede etkin olduğunu tespit ettik. Histopatolojik olarak gösterilen antiinflamatuvar etkinin yanı sıra, biyokimyasal olarak saptanan antioksidan etkinin, Ca-Dob’ın adezyon önleyici etkinliğinde rol oynadığı sonucuna varılmıştır.

Anahtar Kelimeler:

Kalsiyum Dobesilat, Antiinflamatuar, Antioksidan, Adezyon, Deneysel

THE EFFECT OF CALCIUM DOBESILATE ON THE PREVENTION OF EXPERIMENTAL INTRA-ABDOMINAL ADHESIONS

ABSTRACT: OBJECTIVE: It was aimed to demonstrate the efficacy of Calcium Dobesilate (Ca-Dob) in preventing postoperative intra-abdominal adhesion formation in an experimental cecal abrasion model. MATERIALS AND METHODS: Thirty female Wistar Albino rats were randomized into 3 groups. In the sham group, the cecum was removed from the abdomen and replaced without any further processing. In the control group, after cecal abrasion and peritoneal resection no processing was applied. In the Ca-Dob group, after cecal abrasion and peritoneal resection, 100 mg/kg/day Ca-Dob was administered. All rats were sacrificed on 21st day. The evaluation of adhesions was made through the degrees of bowel inflammation and fibrosis in tissue samples and the biochemical analysis of tissue and blood samples. RESULTS: Statistically, inflammation, microscopic adhesions and fibrosis scores were found to be significantly lower in the Ca-Dob administered rats compared to the control group (p < 0.05). Tissue MDA, FOP and TOS values were found to be significantly lower statistically compared to the control group (p = 0.001). Tissue total SH values were found to be statistically significantly higher (p < 0.05) in the sham group compared with the control group. Finally; serum MDA and serum TOS values in the Ca-Dob group, was statistically significantly lower (p < 0.05) compared to control group. CONCLUSION: Ca-Dob was found to be efficacious in preventing the formation of adhesions. In addition to the demonstrated anti-inflammatory effect histopathologically, the effect of antioxidant effect demonstrated biochemically was concluded to participate to the adhesion preventive efficacy of Ca-Dob.

Keywords:

Calcium Dobesilate, Anti-inflammatory, Antioxidant, Adhesion, Experimental,

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