Tülay AŞKIN ÇELİK, Özlem Sultan ASLANTÜRK, Gözde ASLAN, Mesut KIRMACI

Sphagnum divinum Flatberg & K. Hassel ve Sphagnum girgensohnii Russow (Sphagnopsida)'nin Fitokimyasal İçeriklerinin ve Antioksidan Aktivitelerinin Belirlenmesi

Bu çalışmanın amacı, Sphagnum divinum ve S. girgensohnii'den elde edilen metanol ekstraktlarının fitokimyasal bileşenlerini ve antioksidan aktivitesini araştırmaktır. Antioksidan içeriği çok az çalışılmış olan iki farklı Sphagnum türünün metanol ekstraktındaki fitokimyasal aktif madde grupları tarama yöntemleri kullanılarak belirlenmiş ve antioksidan özellikleri in vitro DPPH deneyi, CUPRAC deneyi ve fosfomolibdat deneyi kullanılarak tespit edilmiştir. Deneylerden elde edilen sonuçlara göre, S. divinum 'un metanol ekstraktının sadece fenoller ve tanenler içerdiği, S. girgensohnii 'nin metanol ekstraktının ise fenoller, tanenler ve saponinler içerdiği, ancak her iki briyofitin de alkaloidler ve flavonoidler içermediği belirlenmiştir. Deneysel sonuçlar, S. divinum ve S.girgensohnii metanol ekstraktlarının test edilen tüm konsantrasyonlarda %50'den daha yüksek DPPH süpürme aktivitesine sahip olduğunu göstermektedir. Ekstrakt konsantrasyonundaki artışla birlikte DPPH süpürme kabiliyetinde artış olduğu gözlemlenmiştir. S. divinum metanol ekstraktının DPPH süpürme kabiliyeti, test edilen tüm konsantrasyonlarda S. girgensohnii metanol ekstraktından daha yüksek bulunmuştur. Çalışmanın sonuçları, S.divinum ve S. girgensohnii'nin potansiyel antioksidan bileşik kaynakları olabileceğini göstermektedir.

Anahtar Kelimeler:

Karayosunları, Turbalık, CUPRAC Testi, DPPH, Fosfomolibdat testi

Determination of Phytochemical Content and Antioxidant Activities of Sphagnum divinum Flatberg & K. Hassel and Sphagnum girgensohnii Russow (Sphagnopsida)

This study aimed to explore the phytochemical constituents and antioxidant activity of methanol extracts from Sphagnum divinum and Sphagnum girgensohnii. Screening methods were employed to identify the phytochemical groups present in the methanol extracts of these two Sphagnum species, which have been relatively understudied for their antioxidant potential. The antioxidant properties of the extracts were evaluated using in vitro DPPH, CUPRAC, and phosphomolybdate assays. The experimental results revealed that the methanol extract of S. divinum exclusively contained phenols and tannins, while the methanol extract of S. girgensohnii contained phenols, tannins, and saponins. Alkaloids and flavonoids were not detected in either bryophyte. The methanol extracts of both S. divinum and S. girgensohnii exhibited DPPH scavenging activity exceeding 50% at all tested concentrations. An increase in DPPH scavenging ability was observed with increasing extract concentration. The DPPH scavenging activity of S. divinum methanol extract was consistently higher than that of S. girgensohnii methanol extract across all tested concentrations. These findings suggest that S. divinum and S. girgensohnii hold promise as potential sources of antioxidant compounds.

Keywords:

Bryophytes, Peatland, CUPRAC assay, DPPH, Phosphomolybdate assay,

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