FUSARİUM OXYSPORUM F.SP. CİCERİS’ İN FUSARİK ASİT ÜRETİMİNİN İNCE TABAKA KROMATOGRAFİSİ VE SPEKTROFOTOMETRİK METODLAR İLE BELİRLENMESİ

Öz Bu çalışma Fusarium oxysporum f.sp. ciceris izolatlarının fusarik asit üretimi ve virülenslikleri arasında bir ilişki olup olmadığını belirlemek için yürütülmüştür. F. oxysporum f.sp. ciceris’ in beş izolatı (KC 7, A4, KA6, KC9 ve KC6) Czapek Dox sıvı besin ortamında 5, 10, 15 ve 20 gün süreyle geliştirilmiş ve toxin C18 katı faz ekstraksiyon kartijleriyle ekstrakte edilerek asetonitril ile elue edilmiştir. Asetonitrildeki fusarik asitin varlığı hem ince tabaka kromatografi hem de spektrofotometrik yöntemle belirlenmiştir. Fusarik asitin ince tabaka kromatorafisinde Rf değeri 0.70 olarak bulunmuş ve fluoresan kremit rengi parlaklıkta bir leke vermiştir. Buna ilave olarak ince tabaka kromatografi analizinde bir izolatın tanımlanamayan bir metabolit ürettiği belirlenmiştir (Rf 0.87). Fusarik asitin kantitatif miktar analizi Agilent Specord 200 spectrofotometre ile gerçekleştirilmiştir. İzolatların hiçbirisi inkübasyonun 5. gününde fusarik asit üretmemiştir. Ayrıca düşük ve yüksek virülens izolatların fusarik asit üretimindeki farklılıklar inkübasyonun 20. gününde istatiksel olarak önemli bulunmuştur.

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Anadolu Tarım Bilimleri Dergisi-Cover
  • ISSN: 1308-8750
  • Yayın Aralığı: Yılda 3 Sayı
  • Başlangıç: 1986
  • Yayıncı: Ondokuz Mayıs Üniv. Ziraat Fak.