Bazı GABAA-reseptör modüle edici bitkisel gıdaların antioksidant kapasiteleri

Reaktif oksijenler nörodejeneratif hastalıklar gibi bazı sağlık sorunlarına yol açan oksidatif strese neden olurlar. Bazı antioksidanlar nöroprotektif aktivite gösterir ve GABAA reseptörlerinin pozitif modülasyonu ile nöronal aktiviteyi düzenlerler. Bu çalışmada bazı yeni GABAA reseptör modüle edici bitkisel gıdalardan lavanta, karanfil ve 4 Sideritis türünün (S. arguta, S. sipylea, S. condensata, ve S. stricta) ABTS metodu ile antioksidant etkileri araştırılmıştır. Karanfilin sulu ekstraktı en yüksek antioksidant etki göstermiştir ve IC50 değeri 3.76 μg/mL olarak bulunmuştur. Trolox’un antioksidant kapasitesi (IC50: 4.63 μg/mL) karanfilden düşük ancak diğer bitki ekstraktlarından yüksek bulunmuştur. Sulu lavanta ekstraktı, S. sipylea hariç diğer Sideritis türleri ile karşılaştırıldığında en düşük antioksidant kapasitesi göstermiştir (IC50: 20.75 μg/mL). Sonuç olarak, bu deney sonuçları GABAA reseptör module edici bitkisel gıdaların güçlü antioksidant etkiye de sahip olduklarını doğrulamaktadır.

Antioxidative capacities of some GABAA receptor modulating food plants

Reactive oxygen species cause oxidative stressassociated with some health issues such asneurodegenerative diseases. Some antioxidantsshow neuroprotective activity and regulate neuronalactivity through positive modulation of GABAAreceptors. In this study, the antioxidative capacity ofsome novel GABAA receptor modulating food plants,including lavender, clove and four Sideritis species(S. arguta, S. sipylea, S. condensata, and S. stricta) wasinvestigated using ABTS assay. The aqueous cloveextract showed the most potent antioxidant effectand its IC50 value was found to be 3.76 μg/mL. Theantioxidant capacity of Trolox was lower (IC50: 4.63μg/mL) than that of the clove extract, but higherthan those of the other food extracts. The aqueouslavender extract exhibited less antioxidant activitycompared to Sideritis species, except S. sipylea thathad the least antioxidant capacity (IC50: 20.75μg/mL). In conclusion, these results confirm thatGABAA receptor modulating food plants possessstrong antioxidative activity.

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Alnamer, R., Alaoui, K., Bouidida el, H., Benjouad, A., Cherrah, Y., 2012. Sedative and Hypnotic Activities of the Methanolic and Aqueous Extracts of Lavandula officinalis from Morocco. Advances in Pharmacological Sciences, 2012: 270824.
Alqareer, A., Alyahya, A., Andersson, L., 2006. The effect of clove and benzocaine versus placebo as topical anesthetics. Journal of Dentistry, 34(10): 747-750.
Aoshima, H., Hamamoto, K., 1999. Potentiation of GABAA Receptors Expressed in Xenopus Oocytes by Perfume and Phytoncid. Bioscience, Biotechnology, and Biochemistry, 63(4): 743-748.
Carlsen, M. H., Halvorsen, B. L., Holte, K., Bøhn, S. K., Dragland, S., Sampson, L., Willey, C., Senoo, H., Umezono, Y., Sanada, C., Barikmo, I., Berhe, N., Willett, W. C., Phillips, K. M., Jacobs, D. R., Blomhoff, R., 2010. The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide. Nutrition Journal, 9: 3.
Charimi, M. T., Lazari, D., Karioti, A., Skaltsa, H., Hadjipavlou-Litina, D., Souleles, C., 2008. Antioxidant and antiinflammatory activities of Sideritis perfoliata subsp. perfoliata (Lamiaceae). Phytotherapy Research, 22(4):450-454.
Gonzalez-Burgos, E., Carretero, M. E., Gomez-Serranillos, M. P., 2011. Sideritis spp.: Uses, chemical composition and pharmacological activities - A review. Journal of Ethnopharmacology, 135 (2): 209-225.
Granger, R. E., Campbell, E. L., Johnston, G. A. R., 2005. (+)- And (-)-borneol: efficacious positive modulators of GABA action at human recombinant a1b2g2L GABAA receptors. Biochemical Pharmacology, 69(7): 1101-1111.
Gulcin, I., 2011. Antioxidant activity of eugenol: a structure-activity relationship study. Journal of Medicinal Food, 14(9): 975-985.
Gülçin, İ., Şat, G., Beydemir, Ş., Elmastaş, M., Küfrevioğlu, Ö. İ., 2004. Comparison of antioxidant activity of clove (Eugenia caryophylata Thunb) buds and lavender (Lavandula stoechas L.). Food Chemistry, 87(3): 393-400.
Güvenç, A., Houghton, P.J., Duman, H., Coşkun, M., Şahin, P., 2008. Antioxidant activity studies on selected Sideritis species Native to Turkey. Pharmaceutical Biology, 43(2): 173-177.
Ha J. H., L. D. U., Lee J. T., Kim J. S., Yong C. S., Kim J. A., Ha J. S., Huh K., 2000. 4-Hydroxybenzaldehyde from Gastrodia elata B1. is active in the antioxidation and GABAergic neuromodulation of the rat brain. Journal of Ethnopharmacology, 73(1-2): 329-333.
Halder, S., Mehta, A. K., Mediratta, P. K., Sharma, K. K., 2012. Acute effect of essential oil of Eugenia caryophyllata on cognition and pain in mice. Naunyn-Schmiedeberg's Archives of Pharmacology, 385: 587-593.
Hernandez-Perez, M., Sanchez-Mateo, C. C., MontalbettiMoreno, Y., Rabanal, R. M., 2004. Studies on the analgesic and anti-inflammatory effects of Sideritis candicans Ait. var. eriocephala Webb aerial part. Journal of Ethnopharmacology, 93(2-3): 279-284.
Hosseini, M., Kamkar-Asl, M., Rakhshandeh, H., 2011. Analgesic effect of clove essential oil in mice. Avicenna Journal of Phytomedicine, 1(1): 1-6.
Kessler, A., Sahin-Nadeem, H., Lummis, S. C., Weigel, I., Pischetsrieder, M., Buettner, A., Villmann, C., 2014. GABAA receptor modulation by terpenoids from Sideritis extracts. Molecular Nutrition & Food Research, 58(4): 851-862.
Kessler, A., Villmann, C., Sahin-Nadeem, H., Pischetsrieder, M., Buettner, A., 2012. GABAA receptor modulation by the volatile fractions of Sideritis species used as ‘Greek’ or ‘Turkish’ mountain tea. Flavour and Fragrance Journal, 27(4): 297-303.
Lee, K. J., Oh, Y. C., Cho, W. K., Ma, J. Y., 2015. Antioxidant and anti-inflammatory activity determination of one hundred kinds of pure chemical compounds using offline and online screening HPLC assay. Evidence-Based Complementary and Alternative Medicine, 2015: 165457.
Möhler, H., 2006. GABAA receptors in central nervous system disease: anxiety, epilepsy, and insomnia. Journal of Receptors and Signal Transduction, 26(5-6): 731-740.
Reiter, R. J., 1998. Oxidative damage in the central nervous system: protection by melatonin. Progress in Neurobiology, 56(3): 359-384.
Sahin, S., Eulenburg, V., Heinlein, A., Villmann, C., Pischetsrieder, M., 2017. Identification of eugenol as the major determinant of GABA A -receptor activation by aqueous Syzygium aromaticum L. (clove buds) extract. Journal of Functional Foods, 37: 641-649.
Sahin, S., Eulenburg, V., Kreis, W. Villmann, C., Pischetsrieder, M., 2016. Three-Step Test System for the Identification of Novel GABAA Receptor Modulating Food Plants. Plant Foods for Human Nutrition, 71(4): 355-360.
Sesti, F., Liu, S., Cai, S. Q., 2010. Oxidation of potassium channels by ROS: a general mechanism of aging and neurodegeneration? Trends in Cell Biology, 20(1): 45-51.
Shaw, D., Annett, J. M., Doherty, B., Leslie, J. C., 2007. Anxiolytic effects of lavender oil inhalation on open-field behaviour in rats. Phytomedicine, 14(9): 613-620.
Sigel, E., Steinmann, M. E., 2012. Structure, function, and modulation of GABAA receptors. Journal of Biological Chemistry, 287(48): 40224-40231.
Silva, M. I., Silva, M. A., de Aquino Neto, M. R., Moura, B. A., Sousa, H. L., de Lavor, E. P. H., de Vasconcelos, P. F., Macêdo, D. S., de Sousa, D. P., Vasconcelos, S. M., de Sousa, F. C., 2009. Effects of isopulegol on pentylenetetrazol-induced convulsions in mice: possible involvement of GABAergic system and antioxidant activity. Fitoterapia, 80(8): 506-513.
Silva, G. L., Luft, C., Lunardelli, A., Amaral, R. H., Melo, D. A., Donadio, M. V., Nunes, F.B., de Azambuja, M. S., Santana, J. C., Moraes, C. M., Mello, R. O., Cassel, E., Pereira, M. A., de Oliveira, J. R., 2015. Antioxidant, analgesic and anti-inflammatory effects of lavender essential oil. Anais da Academia Brasileira de Ciências, 87(2):1397-1408
Willcox, J. K., Ash, S. L., Catignani, G. L., 2004. Antioxidants and prevention of chronic disease. Critical Reviews in Food Science and Nutrition, 44(4): 275-295.
Woronuk, G., Demissie, Z., Rheault, M., Mahmoud, S., 2011. Biosynthesis and therapeutic properties of Lavandula essential oil constituents. Planta Medica, 77(1): 7-15.
Wu, C., Gopal, K. V., Moore, E. J., Gross, G. V., 2014. Antioxidants L-carnitine and D-methionine modulate neuronal activity through GABAergic inhibition. Journal of Neural Transmission, 121(7): 683-693.
Zbarsky, V., Datla, K. P., Parkar, S., Rai, D. K., Aruoma, O. I., Dexter, D. T., 2005. Neuroprotective properties of the natural phenolic antioxidants curcumin and naringenin but not quercetin and fisetin in a 6- OHDA model of Parkinson's disease. Free Radical Research, 39(10): 1119-1125.