Lütfi BEHÇET, A. Adnan HAYALOĞLU, Bayram YURT, Nurullah DEMİR

LOKAL ENDEMİK RIBES ANATOLICA BEHÇET (GROSSULARIACEAE) MEYVELERİNİN BAZI FİZİKOKİMYASAL ÖZELLİKLERİ, TOPLAM FENOLİK MADDE MİKTARI, ANTİOKSİDAN KAPASİTELERİ VE FENOLİK KOMPOZİSYONUNUN BELİRLENMESİ

Bu çalışmada, yeni bir tür olan Ribes anatolica Behçet türünün yabani meyvelerinin nem miktarı % 74.69 ± 1.03, kuru madde miktarı % 25.31 ± 1.03, su aktivitesi(aw) 0.948 ± 0.001, Suda çözünen kuru madde miktarı (Briks) 17.96 ± 1.31, pH 4.120 ± 0.013, Titrasyon asitliği 0.94 ± 0.07 (g sitrik asit / 100g), Yüzey rengi değerleri L* 57.79 ± 0.85, a* 10.19 ± 1.70, b* 4.03 ± 1.34, C* 10.98 ± 2.03 ve h° 21.13 ± 3.80, Toplam fenolik madde miktarı 11.37 ± 0.88 (mg G.A.E./ 100g kuru ağırlık) ve Antioksidan kapasitelerini gösteren ABTS değerleri 314.85 ± 52.97 (µmol T.E. / 100g kuru ağırlık) ve DPPH değerleri 213.24 ± 20.32 (µmol T.E. / 100g kuru ağırlık), Fenolik bileşikler µg/g oarak; Gallik asit 3.51±1.86, Protocatechuic asit 22.80±5.88, 4-dihydroxy benzoik asit 19.17±4.89, Syringik asit 13.52±4.29, Trans-cinnamik asit 31.92±18.22, Chlorogenik asit 22.69 ±0.36, Rutin 152.47±17.32, Quercetin-3-O-glucoside 114.71±2.99 ve Kaempferol-3-O-glucoside 32.13 ±2.35 düzeyinde bulunmuştur.

DETERMINATION OF SOME PHYSICOCHEMICAL FEATURES , ANTIOXİDANT CAPACITIES, TOTAL PHENOLIC CONTENT AND PHENOLIC COMPOSITION OF LOCAL ENDEMIC RIBES ANATOLICA BEHÇET (GROSSULARIACEAE) FRUITS

In this study, a new species, Ribes anatolica Behçet, analysis mean values were found as the following; moisture content 74.69 % ± 1.03, dry matter 25.31% ± 1.03, water activity (aw) 0.948 ± 0.001, water soluble solids content(Brix) 17.96 ± 1.31, pH 4.120 ± 0.013, titratable acidity 0.94 ± 0.07 (g citric acid / 100g), surface color values L* 57.79 ± 0.85, a* 10.19 ± 1.70, b* 4.03 ± 1.34, C* 10.98 ± 2.03 and h° 21.13 ± 3.80, Total amount of phenolic substance 11.37 ± 0.88 (mg G.A.E./ 100g dry matter), showing antioxidant capacities, ABTS values 314.85 ± 52.97 (µmol T.E. / 100g dry matter) and DPPH values 213.24 ± 20.32 (µmol T.E. / 100g dry matter), Phenolic compounds in µg/g ; Gallic acid 3.51±1.86, Protocatechuic acid 22.80±5.88, 4-dihydroxy benzoic acid 19.17±4.89, Syringic acid 13.52±4.29, Trans-cinnamic acid 31.92±18.22, Chlorogenic acid 22.69 ±0.36, Rutin 152.47±17.32, Quercetin-3-O-glucoside 114.71±2.99 and Kaempferol-3-O-glucoside 32.13 ±2.35.

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[1] Efe R. 2010. Biocoğrafya. Marmara Kitap Merkezi Yayıncılık Ltd. Şti. No: 16215. Bursa.
[2] Heywood, V.H., Brummitt, R. K., Culham, A., Seberg, O. (2007). Flowering Plant Families of The World. Firefly Books pres, Ontario, Canada, p.: 160.
[3] Karaer, F., Adak, Y. Turkiye florasında uzumsu meyve olarak kullanılan taksonların yayılış alanları ve ekolojik ozellikleri. 2006. II. Ulusal Üzümsü Meyveler Sempozyumu, Bildiriler: 36-43, 14-16 Eylul 2006 /Tokat.
[4] Ames, B.N., Shigenaga, M.K., Hagen, M.T. Oxidants, antioxidants and the degenerative diseases of aging. 1993. Proc. Natl. Acad. Sci.,90: 7915-7922.
[5] Kadiiska, M.B., Gladen, B.C., Baird, D., Graham, I., Parker, C. and Ames, B.. Biomarkers of oxidative stress study III. Effects of the nonsteroidal antiinflammatory agents indomethacin and meclofenamic acid on measurements of oxidative products of lipids in CCl4 poisining. 2005. Free Rad. Bio. Med., 38:711-718.
[6] Mates, J.M., Sanchez-Jimenez, F.M. Role of oxygen species inapoptosis: implications for cancer therapy. 2000. International Journal of Biochemistry and Cell Biology, 32: 157–170.
[7] Pellegrini, N., Miglio, C., Delrio, D. Effect of domestic cooking methods on the total antioxidant capacity of vegetables. 2009. International Journal of Food Sciences and Nutrition 60 (Suppl 2): 12–22.
[8] Karakaya S, El S, Tas AA. Antioxidant activity of some foods containing phenolic compounds. 2001. International Journal of Food Sciences and Nutrition; 52: 501-508
[9] Özgen, M., Serçe, S., Kaya, K. Phytochemical and antioxidant properties of anthocyanin-rich Morus Nigra and Morus Rubra fruits. 2009. Scientia horticulturae,119: 275-279.
[10] Eyduran, S. P., Ağaoğlu, Y. S. Ankara (Ayaş) koşullarında yetiştirilen frenk uzumu ceşitlerinin bazı pomolojik ve bitkisel ozellikleri. 2007. Ankara Universitesi Ziraat Fakultesi, Tarım Bilimleri Dergisi, 13 (3): 293-298.
[11] Miller, N.J., Paganga, G. Structureantioxidant activity relationships of flavonoids and phenolic acids. 1996. Free Radical Biology & Medicine, 20: 933-956.
[12] Scheerens, J.C. Phytochemicals and the consumers: factors affecting fruit and vegetable consumption and the potential for increasing small fruit in the diet. 2001. Horttech, 11:547-556.
[13] Kökosmanlı, M., Keleş, F. Erzurum’da yetiştirilen kızılcık meyvesinin marmelat ve pulpa işlenerek değerlendirilmesi. 2000. Gıda 25(4): 289-298.
[14] Kendir, G. Türkiye’de doğal olarak yetişen Ribes L. Türleri Üzerinde farmasötik botanik yönünden Araştırmalar. 2012. Doktora Tezi, Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, 359s. Ankara.
[15] Chamberlain D.F. Ribes L. In Davis P.H. (ed.) Flora of Turkey and The East Aegean Islands. 1972. Edinburgh Universty Press, vol. 4: 261-263.
[16] Behcet, L. A new species of Ribes L. (Grossulariaceae) from east Anatolia, Turkey. 2001. Turk. J. Botany., 25: 103-105.
[17] Kılıç, C. S., Koyuncu, M., Ozek, T., Başer, K. H. C. (2008). Essential oil of the leaves of Ribes nigrum L. from Turkey. Journal of Essential Oil Research, 20(6): 512-514.
[18] AOAC. 1990. Official Method 979.23: Saccharides (major) in corn syrup.
[19] Karabulut, İ., Bilenler, T., Sislioglu, K., Gökbulut, İ., Özdemir,İ.S., Seyhan, F., Özturk, K. Chemical composition of apricots affected by fruit size and drying methods, Drying Technology, 2018. 36:16, 1937-1948.
[20] Cemeroğlu, B. Gıda analizleri. 2010. Gıda Teknolojisi Derneği Yayınları, No:34. 657s.
[21] Singleton, V.L., Orthofer, R., Lamuela-raventós, R.M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. 1999. Methods in Enzymology, 299: 152-178.
[22] Kendir, G., Koroğlu, A. In vitro antioxidant effect of the leaf and branch extracts of Ribes L. species in Turkey. 2015. Int. J. Pharma Sci. Res. 2, 108.
[23] Tabart, J., Franck, T., Kevers, C., Pincemail, J., Serteyn, D., Defraigne, J.-O., Dommes, J. 2012. Antioxidant and anti-inflammatory activities of Ribes nigrum extracts. Food Chem. 131, 1116– 1122.
[24] Sasaki, T., Li, W., Zaike, S., Asada, Y., Li, Q., Ma, F., Zhang, Q., Koike, K., 2013. Antioxidant lignoids from leaves of Ribes nigrum. Phytochemistry 95, 333–340.
[25] Cyboran, S., Bonarska-Kujawa, D., Pruchnik, H., Zyłka, R., Oszmianski, J., Kleszczynska, H., 2014. Phenolic content and biological activity of extracts of blackcurrant fruit and leaves. Food Res. Int. 65, 47–58.
[26] Wu, X., Gu, L. Prior, R.L., Mckay, S. J. Characterization of Anthocyanins and Proanthocyanidins in Some Cultivars of Ribes, Aronia, and Sambucus and Their Antioxidant Capacity.2004. Agric. Food Chem., 52, 7846−7856.
[27] Yazdi, M. E.T., Khara, J., Husaindokht, M. R., Sadeghnia, H. R., Bahabadi, S. E.,Amiri, M.S., Darroudi, M. Biocomponents and Antioxidant Activity of Ribes khorasanicum. 2018. International Journal of Basic Science in Medicine. 3:(3), 99-103.
[28] Kendir, G., Süntar İ., Çeribaşı, A. O., Köroğlu, A. Activity evaluation on Ribes species, traditionally used to speed up healing of wounds: With special focus on Ribes nigrum. 2019. Journal of Ethnopharmacology 237: 141–148.