THE ASSOCIATION OF THE CARCİNOGENİC EFFECT OF N-NİTROSO-N-METHYLUREA WİTH THE BLOOD PLASMA VİTAMIN E AND SELENİUM LEVELS İN RATS

AbstractEffects of vitamin E and Se on cancer induced by N-N'itroso-N-Methylurea. which is a chemical carcinogen were investigated in this study. The study was carried out with 50 female Wistar-AIbino rats. The animals were divided into five groups: Four experimental and 1 control (n: 10). The animals in groups 1 and II were daily given 3ppm/lt sodium sclcnit via drinking water starting 45 days prior to NMU injection, while groups II and IV were injected vitamin E intraperitoneally (i.p) three times a week. At the end of the 45-day-period blood samples were taken from each animal in individual group via tail veins and the experimental groups (1-4) were administrated 20 mg/kg of NMU intraperitoneally three times a week during the second 45-day-period. After this stage vitamin E and Se application was prolonged for ¡70 days (experimental stage totally lasted for 260 days). All animals were sacrificed at the end of the study after the blood samples were obtained. Histologically, tumourai mass development was obtained in one (10%), five (50%), two (20%) and two animals (20%) in groups I (Se+NMU). II (Se+Vit.E+NMU). Ill (Vit.E+NMU) and IV (NMU), respectively. In conclusion, the ratio of tumourai development in group I. which was slightly lower than in group I pointed out at least the partial effectiveness of Se on prevention of neoplastic development. On the other hand, vitamin E was demonstrated to be ineffective in the prophylaxis of tumourai development due to equal tumour incidences detected in groups III (Vit.E) and IV (Only NMU). The ratio of tumourai development in group II (Se+Vit.E) was detected to be significantly higher than the other groups. Serum Se and vit.E levels in the relevant group were demonstrated to be lower than in group I (despite the insignificance of the estimated value) and significantly higher (p<0.05) than in group III. respectively, which all together indicated the likelihood of the inductive role of high serum levels of vit.E in tumour development.Key Words: Vitamin E, Selenium, N-Nitroso-N-Methylurea, Cancer, Rat SIÇANLARDA N-NİTROSO-N-METHYLUREA'NIN KARSİNOJEN ETKİSİNİN KAN PLAZMASINDAKİ E VİTAMİNİ VE SELENYUM DÜZEYLERİYLE İLİŞKİSİ ÖzetBu çalışmada kimyasal bir karsinojen olan N-Nitroso-N-Methylurea (NMU) ile oluşturulan kanserlerde E vitamini (Vit.E) ve selenyumun (Se) etkisi araştınldı.Çalışmada 50 adet VVistar-albino dişi sıçan kullanıldı. Hayvanlar dört deney ve bir kontrol grubu olmak üzere beş gruba ayrıldı (n=10). Bir ve ikinci gruplara NMU enjeksiyonundan 45 gün önce başlanarak içme sularına günlük 3ppm/lt sodyum selenit eklendi. İki ve üçüncü gruplara aynı süre içerisinde haftada üç kez Vit.E intraperitoneal (i.p) uygulandı. Belirtilen sürenin sonunda bütün gruplardaki hayvanların kuyruk venalanndan kan alındı ve deney gruplarına (1-4) 45 gün boyunca haftada üç kez 20mg/kg NMU i.p uygulandı. Bu uygulamanın bitiminden çalışmanın sonuna kadar 170 gün daha (toplam 260 gün) Vit.E ve Se uygulamasına devam edildi.Çalışmanın sonunda hayvanlardan tekrar kan alındıktan sonra ötanazi edilerek nekropsileri yapıldı.Histopatolojik inceleme sonunda birinci grupta (Se+NMU) bir hayvanda (%10), ikinci grupta (Se+Vit.E+NMU) beş hayvanda (Çi-50), üçüncü (VİLE+NMU) ve dördüncü grupta (NMU) ikişer hayvanda {7c20) tümör oluşumu saptandı.Sonuç olarak Sc verilen birinci gruptaki tümör oranının dördüncü gruba göre birazda olsa düşük çıkması Se'un tümör oluşumunu engellemede kısmen de olsa etkili olduğunu, Vit.E verilen üçüncü grup ile yalnızca NMU verilen dördüncü grupta tümör oranlarının eşit çıkması nedeniyle de Vit.E'nm tümör oluşumunu engellemede etkisiz olduğunu göstermektedir. Ancak Se+Vit.E verilen ikinci gruptaki tümör oranının diğer gruplardan belirgin olarak yüksek çıkması, aynca bu gruptaki serum Se düzeyinin istatistiksel olarak anlamlı olmamakla birlikte birinci gruba göre düşük, serum Vit.E düzeyinin ise üçüncü gruba göre belirgin olarak yüksek (p<0.05) çıkması nedeniyle, serumda yüksek düzeyde bulunan Vit.E'nin tümör oluşumunu provake edebileceğini düşündürmektedir.Anahtar Kelimeler: E vitamini, Selenyum, N-Nitroso-N-Methylurea, Kanser, Sıçan

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