Mukaddes PALA, Mehmet ALTAN, Ferahat HANİKOGLU, Salim NESELİOGLU, Özcan EREL, Gökhan METİN

Düzenli olarak uygulanan orta şiddetteki egzersiz programının tiyol/disülfid homeostazı ve iskemi modifiye albümin üzerine etkisi

Amaç: Tiyol/disülfid homeostazı, oksidatif stresin ve antioksidan kapasitenin bir göstergesidir. İskemi-modifiye albümin (İMA), hem oksidatif stres hem de iskemi için önemli bir belirteçtir. Düzenli olarak uygulanan orta şiddetteki egzersizin tiyol/disülfid homeostazı ve IMA seviyeleri üzerine olası etkilerini değerlendirmeyi amaçladık.Yöntemler: Sprague-Dawley sıçanlar kullanıldı. Çalışma, Egzersiz grubu (EG, n=9) ve Kontrol grubundan (KG, n=6) oluşturuldu. 10 haftalık bir yüzme egzersizi yaptırıldı. Bu çalışmada tiyol/disülfid homeostazı ölçüm yöntemi kullanıldı. İMA seviyeleri, bir kobalt-albümin bağlama yöntemiyle ölçüldü.Bulgular: EG'de, total tiyol seviyeleri KG ile karşılaştırıldığında anlamlı derecede daha yüksekti (p<0.01). Disülfid/total tiyol oranı EG’da KG ile karşılaştırıldığında daha düşüktü (p<0.01). EG'da IMA seviyelerinde hafif bir artış olduğunu gözlemledik (p=0,18). Bu artış istatistiksel olarak anlamlı değildi.Sonuç: Düzenli olarak uygulanan orta şiddetteki egzersiz, nativ ve total tiyol seviyelerini artırdı. Tiyol seviyelerinin artması oksidatif stresi önleyebilir. Düzenli olarak uygulanan orta şiddetteki egzersiz programlarının oksidatif stres üzerinde olumlu etkiler sağladığı görülmektedir.

Anahtar Kelimeler:

Yüzme, oksidatif stres, tiyol-disülfit homeostazı, iskemi-modifiye albumin

The effect of regularly performed moderate-intensity exercise program on thiol/disulfide homeostasis, and ischemia-modified albumin

Aim: Thiol/disulfide homeostasis is an indicator of oxidative stress and antioxidant capacity. Ischemia-modified albumin (IMA) is an important marker for both oxidative stress and ischemia. We aimed to evaluate the possible effects of regularly performed moderate-intensity exercise on thiol/disulfide homeostasis, and IMA levels.Methods: Sprague Dawley rats were used. The study was composed of an Exercise group (EG, n=9) and Control group (CG, n=6). A 10-weeks swimming exercise was performed. Thiol/disulfide homeostasis measurement method was used in this study. IMA levels were measured by a cobalt-albumin binding method.Results: In the EG, total thiol levels were significantly higher compared to the CG (p<0.01). The disulfide/total thiol ratio was lower in the EG compared to the CG (p<0.01). We observed that there was a slight increase in IMA levels in EG (p=0.18). This increase was not statistically significant.Conclusion: Regularly performed moderate-intensity exercise has increased native and total thiol levels. Increase of thiol levels can prevent oxidative stress. Regularly performed moderate-intensity exercise programs appear to provide favourable effects on oxidative stress.

Keywords:

Swimming, oxidative stress, thiol/disulfide homeostasis, ischemia-modified albumin,

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