Osteointegre Dental İmplant Tedavisi Gören Hastalarda IL-1 β Genotipi ve Seviyelerinin İncelenmesi

DOI: 10.26650/experimed.2019.19002Amaç: Çalışmamızda osteointegre dental implant kayıplarında interlökin-1β (IL-1β) +3953 gen polimorfizminin serum interlökin düzeyleri ve sigara içimiyle ilişkisini araştırmayı amaçladık. Yöntemler: İmplant yapılan 56 olgu ve 238 sağlıklı kontrol öalışmaya dahil edilmiştir. IL-1β +3953 polimorfizmi, polimeraz zincirleme reaksiyonu ve restriksiyon fragmanı uzunluk polimorfizmi yöntemleri kullanılarak tayin edilmiştir. Serum örneklerinde IL-1β seviyesi ELISA yöntemi ile saptanmıştır.Bulgular: IL-1β +3953 polimorfizmi; CC, TT ve CT genotip frekansları kontrol ve hasta grubu için sırasıyla 61.2%, 10.1% , 43.6%, ve 48.2%, 8.9% ve 42.9% olarak bulunmuştur. IL-1β +3953 T alleli taşıyanların hastalarda (51.8%) kontrol grubuna (38.8%) göre daha yüksek bulunmuştur ve bu kişilerde plak indeksi, kanama indeksi, cep derinliği ve serum IL-1β düzeylerinin arttığı, sigara içme ve implant kaybı oranının yükseldiği fakat istatistiksel anlamlılığa ulaşmadığı tespit edilmiştir (p>0,05). İmplant kaybında, C alleli taşıyanlarda T alleli taşıyanlara göre daha anlamlı bulunmasına rağmen istatistiksel olarak anlamlı olmadığı görülmüştür (p>0,05). İmplant kaybı olan beş hastanın üçünde IL-1β pozitif olduğu, hepsinin sigara kullandığı, implantların erken dönemde kaybedildiği, plak ve kanama indekslerinin ortalama değerlerden yüksek olduğu ve cep derinliklerinin normal sınırlar arasında olduğu görülmüştür. Sonuç: Sonuç olarak implant kaybında IL-1β pozitif genotipe sahip olma ve sigara içiminin önemli bir etken olduğu izlenimi edinilmiş ancak olgu sayısının arttırılmasıyla istatistiksel anlamlılığa ulaşılabileceği sonucuna ulaşılmıştır.Cite this article as: Dizen F, Görmüş U, Ergen A, Ünür M, İplik ES, Çakmakoğlu B, et al. Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated Dental Implants. Experimed 2019; 9(1): 11-5.

Anahtar Kelimeler:

IL-1β +3953, implant, interlökin, polimorfizm

Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated Dental Implants

DOI: 10.26650/experimed.2019.19002Objective: In our study, we aimed to investigate the relationship between the Interleukin-1β (IL-1β) +3953 gene polymorphism, the levels of this interleukin and smoking in osteointegrated dental implant losses. Materials and Methods: 56 patients with implants and 238 healthy control subjects were included in this study. PCR and RFLP methods was used to determine the IL-1β +3953 gene polymorphisms. Serum IL-1β levels were measured using ELISA. Results: The IL-1β +3953 CC, TT and CT genotype frequencies for the controls and study cases were 61.2%, 10.1% ,43.6%, and 48.2%, 8.9% and 42.9%, respectively. IL-1β +3953 T allele carriers increased more in the patient group (51.8%) compared to the control group (38.8%). These cases also had an increased plaque index, bleeding index, pocket deepness and serum IL-1β levels, but none of them were statistically significant (p>0.05). Implant losses were more frequent in C allele carriers than in T allele carriers, but this was not statistically significant (p>0.05) Three of the five patients that experienced implant loss and smoked, lost the implant earlier and had a higher average plaque and bleeding index.Conclusion: Based on our results, we can suggest that being an IL-1β positive genotype and a smoker can have an effect on implant losses. The study group should be enlarged to comment with statistically significant results. Cite this article as: Dizen F, Görmüş U, Ergen A, Ünür M, İplik ES, Çakmakoğlu B, et al. Investigation of the IL-1 β Genotype and its Levels on Patients Treated with Osteointegrated Dental Implants. Experimed 2019; 9(1): 11-5.

Keywords:

IL-1β +3953, Implant, Interleukin, polymorphism,

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