Deneysel Diyabette Döteryum Azlığının Eritrositler Üzerine Etkisi

Diabetik komplikasyonların gelişmesinde, hiperglisemi kaynaklı oksidatif stres çok önemli bir rol oynamaktadır. Döteryum azlığının hücresel hasara karşı ve tümör baskılayıcı etki üzerine koruyucu etkisi olduğu kanser hücre hatlarında gösterilmiştir. Çalışmanın amacı, döteryum azaltılmasının sıçanlarda streptozotosin (STZ) ile oluşturulmuş deneysel diabette eritrosit oksidan ve antioksidan sistem parametreleri üzerindeki etkisinin araştırılması ve değerlendirilmesidir. STZ enjeksiyonunu takiben 30. veya 40. günde, kan örnekleri alınıp, kan glukoz değerleri ölçülmüş, eritrosit hemolizatları da hazırlanmıştır. Eritrositlerin lipid peroksidasyon ve glutatyon seviyeleri ile glutatyon-S-transferaz, süperoksit dismutaz ve katalaz enzim aktiviteleri tayin edilmiştir. 30 veya 40 günlük periyotlardaki döteryumu azaltılmış su tüketiminin, eritrosit oksidan-antioksidan parametre düzeylerine etkisinin farklı olduğu görülmüştür. İçme suyunda döteryumun 150 ppm den 85 ppm’e azaltılmasının net etkisi glutatyon düzeylerinde gözlenmiş olup hem kontrol hem de diyabetik gruplarda eritrosit glutatyon düzeylerini artırdığı saptanmıştır. Sonuç olarak, döteryumu azaltılmış suyun, diabete bağlı oksidatif hasara karşı koruyucu etkisi olabilir. Döteryumu azaltılmış suyun etki mekanizmasının, uygun dozunun ve uygulama süresinin aydınlatılması için daha fazla çalışmalara ihtiyaç vardır.

Anahtar Kelimeler:

Deneysel diabet, eritrosit, döteryum azlığı, antioksidan ve oksidan parametreler

Effect of Deuterium Depletion on Erythrocytes in Experimental Diabetes

In the development of diabetic complications, oxidative stress caused by hyperglycemia plays an important role. Deuterium depletion has protective effect against cellular damage and tumor suppressor effect in cancer cell lines. The purpose of this study was to examine and evaluate the effect of deuterium depletion on erythrocyte oxidant and antioxidant system parameters in experimental diabetes induced by streptozotocin (STZ) in rats. On the 30th or 40th day after STZ injection, blood samples were taken and blood glucose levels were measured. Erythrocyte hemolysates were also prepared. Lipid peroxidation and glutathione levels and glutathione-S-transferase, superoxide dismutase and catalase enzyme activities of erythrocytes were determined. At the 30 or 40 day periods, deuterium depleted water (DDW) consumption had differently affected oxidant-antioxidant parameters of erythrocytes. The clear effect of deuterium depletion in drinking water from 150 ppm to 85 ppm was observed in glutathione levels and it increased erythrocyte glutathione levels in both the control and diabetic groups. In conclusion, deuterium depletion may have protective effects on diabetes induced oxidative stress. Further studies are needed to be done to determine the mechanism of action and the useful dosage and the application period of DDW.

Keywords:

Experimental diabetes, erythrocyte, deuterium depletion, antioxidant and oxidant parameters,

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