Yıl 2020, Cilt: 50 Sayı : 1 Sayfalar 16 - 20 2020-04-01
Core shell column in high-performance liquid chromatography for the determination of polar compounds; troxerutin and carbazochrome
Merve KEŞKEK ARSLAN,Durişehvar ÖZER ÜNAL
13 261

Öz Background and Aims: The combination of troxerutin and carbazochrome has been found efficacious for non-surgical patients with acute uncomplicated hemorrhoids. Therefore it is essential to develop a simultaneous analysis method of those drug substances. This study explains a simple and selective HPLC method to analyze troxerutin and carbazohrome in tablets. Methods: The analysis was carried out using a core-shell pentafluorophenyl propyl polar column with a mobile phase consisting of (MeOH; % 5 HAc), (99.5:0.5 - V/V) at a flow rate of 0.25 mL/min. UV detection was set to 350 nm. Results: The standard calibration curve was established between 1.00-20.00 μg/mL for troxerutin; 0.05-1.00 μg/mL for carbazochrome. LOD and LOQ were found to be 0.65 μg/mL and 1.00 μg/mL for troxerutin and 0.01 μg/mL and 0.05 μg/mL for carbazochrome, respectively. Conclusion: The proposed method was validated for specificity, linearity, accuracy, precision, LOQ and LOD and then successfully applied for the analysis of these substances in tablets.

Anahtar Kelimeler

Troxerutin, carbazochrome, high performance liquid chromatography, core-shell column, tablet
• Abdelrahman, M. M., Abdelaleem, E. A., Ali, N. W., & Emam, R. A. (2016). Simultaneous determination of Carbazochrome and Troxerutin in their binary mixture by HPLC and HPTLC-Densitometric methods. Bulletin of Faculty of Pharmacy, Cairo University, 54(1), 67–75.
Birincil Dil en
Konular Farmakoloji ve Eczacılık, Sağlık Bilimleri ve Hizmetleri
Yayımlanma Tarihi 1 Nisan 2020
Bölüm Original Article
Yazarlar Orcid : 0000-0002-5758-9528Yazar: Merve KEŞKEK ARSLAN (Sorumlu Yazar)Kurum: ISTANBUL UNIVERSITYÜlke: Turkey

Orcid : 0000-0003-0754-1240Yazar: Durişehvar ÖZER ÜNAL Kurum: ISTANBUL UNIVERSITYÜlke: Turkey

Tarihler

Yayımlanma Tarihi : 1 Nisan 2020


Makalenin Yazarları
Merve KEŞKEK ARSLAN
Durişehvar ÖZER ÜNAL