Yıl 2019, Cilt: 1 Sayı : 1 Sayfalar 30 - 41 2019-08-19
Staphylococcus aureus and Staphylococcal enterotoxin detection in raw milk and cheese origin coagulase positive isolates
Tuba YILDIRIM,Farid SADATİ,Berna KOCAMAN,Belgin SİRİKEN
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Öz In the present study, a total 110 (60 cheese and 50 cows’ raw milk) samples was analyzed for CPS according to Food Drug Administration (FDA, 2001) previously. After the isolation, to confirmation of the isolates, catalase test, microscopic examination, coagulase test in tube and glucose-mannitol fermentation tests were applied. After the tests, we obtained 97 CPS isolates. The 97 CPS isolates were used as a material. For the confirmation of the isolates being Staphylococci species, 16S rRNA was detected by using PCR assay. For the detection of the CPS isolates being S. aureus, nuc gene detected in the CPS isolates using PCR assay. To determination of the staphylococcal enterotoxin, PCR assay was applied. The 16 S rRNA was detected in a total 97 (35 milk origin and 62 cheese origin) isolates. Therefore, these isolates were evaluated as coagulase-positive staphylococci (CPS).  The nuc gene was detected in 50 out of 97 CPS isolates. So, the 50 (18 cheese origin and 32 milk origin) isolates were evaluated as S. aureus. However, none of the staphylococcal enterotoxin genes (SE A,B,C,D,E,G,H,I,J) was detected in 97 CPS or 50 S. aureus isolates.

Anahtar Kelimeler

Food Contamination, Bacterial Toxin, CPS, nuc Gene
Argudin, M.A., Mendoza, M.C., & Rodicio, M.R. 2010. Food poisoning and Staphylococcus aureus enterotoxins, Toxins, 2: 1751-1773.
Birincil Dil en
Konular Temel Bilimler
Yayımlanma Tarihi 19 Ağustos 2019
Bölüm Research Articles
Yazarlar Orcid : 0000-0001-8575-4802Yazar: Tuba YILDIRIM (Sorumlu Yazar)Kurum: AMASYA UNIVERSITYÜlke: Turkey

Orcid : 0000-0003-3760-1590Yazar: Farid SADATİ Kurum: AMASYA UNIVERSITYÜlke: Turkey

Orcid : 0000-0002-5578-8697Yazar: Berna KOCAMAN Kurum: AMASYA UNIVERSITY

Orcid : 0000-0002-5793-1792Yazar: Belgin SİRİKEN Kurum: ONDOKUZ MAYIS UNIVERSITY

Tarihler

Yayımlanma Tarihi : 19 Ağustos 2019


Makalenin Yazarları
Tuba YILDIRIM
Farid SADATİ
Berna KOCAMAN
Belgin SİRİKEN