Farklı Gelişme Kuvvetine Sahip 14 Elma Anacının Yapraklarında Fenolik Bileşenlerin Değişimi

Bu çalışmada, 2014 yılında, farklı gelişme kuvvetlerine sahip 14 elma anacının yapraklarında, fenolik bileşenlerin miktarının belirlenmesi amaçlanmıştır. Araştırmada; P.16, P.22, M.9, B.469, O.3, Mac.9, M.26, Pi.80, M.7, M.2, B.118, MM.111, M.25 ve A.2 anaçları yer almıştır. Yaprak örnekleri temmuz ayında sürgünlerin orta yerinden alınmıştır. Gallik asit, ferulik, asit, p-kumarik asit, 4hidroksibenzoik asit, phloridzin ve quersetin miktarları Yüksek Performanslı Sıvı Kromatografi (HPLC) tekniği ile saptanmıştır. Fenolik bileşenler üzerine anaç etkisi önemli çıkmıştır. Elde edilen sonuçlara göre gallik asit 0.796 (P.16) - 88.536 (O.3) mg/kg; 4-hidroksibenzoik asit 24.28 (B.469) 202.13 (M.7) mg/kg; p-kumarik asit 62.35 (M.9) - 181.15 (M.26) mg/kg; ferulik asit 5.21 (M.25) 35.59 (P.22) mg/kg; phloridzin 20.41 (B.469) - 149.59 (M.26) mg/kg ve quersetin 11.56 (B.469)61.33 (M.2) mg/kg arasında değişmiştir. İncelenen fenolik bileşenlerin toplamı dikkate alındığında; M.26 ve M.2 anaçlarının yaprakları en yüksek değerlere sahip olurken, B.469 ve M.9 anaçları en düşük değerleri göstermişlerdir. Fenolik bileşenler ile gelişme kuvveti arasında anlamlı bir ilişki görülmemiştir.

Variation of Phenolic Content in Leaves of 14 Apple Rootstocks with Varying Growth Vigour

The aim of the study, 2014, was to determine the concentration of the main phenols in the leaves of 14 apple rootstocks which has different growth vigour. These are P.16, P.22, M.9, B.469, O.3, Mac.9, M.26, Pi.80, M.7, M.2, B.118, MM.111, M.25 and A.2. The samples of leaves were taken the middle part of the shoots in July. High-performance liquid chromatograph (HPLC) technique was used to quantify the phenols: gallic acid, ferulic acid, p-coumaric acid, 4-hydroxybenzoic acid, quercetin and phloridzin. The contents of on phenolic components were affected by the rootstock. According to data the gallic acid was between 0.796 (P.16) and 88.536 (O.3) mg/kg FW; the 4hydroxbenzoic acid was between 24.28 (B.469) and 202.13 (M.7) mg/kg FW; the p-cumaric acid was between 62.35 (M.9) and 181.15 (M.26) mg/kg FW; the ferulic acid was between 5.21 (M.25) and 35.59 (P.22) mg/kg FW; the phloridzin was between 20.41 (M.9) and 149.59 (M.26) mg/kg FW and the quercetin was between 11.56 (B.469) and 61.33 (M.2) mg/kg FW. Considering the total amount of phenolic compounds investigated the leaves of M.26 and M.2 rootstocks had the highest polyphenolic concentration in total, while the leaves of B.469 and M.9 rootstocks had the lowest. There was no a significant relationship between the components of phenolic and growth vigour.

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