Fatma Gül GÖZE ÖZDEMİR, Bekir TOSUN, Arif ŞANLI, Tahsin KARADOĞAN

Türkiye’de Yetişen Bazı Apiaceae Türlerinin Uçucu Yağlarının Kök Lezyon Nematodlarına Karşı Nematisidal Aktiviteleri

Çalışmada Türkiye doğal florasında yetişen Apiaceae familyasına ait 12 farklı bitki türünün uçucu yağının Kök lezyon nematodlarına (Pratylenchus penetrans, P. thornei ve P. neglectus) karşı nematisidal etkileri incelenmiştir. Araştırma in vitro koşullarda yürütülmüş olup, her bir uçucu yağın 1000 ppm konsantrasyonu kullanılmıştır. Uçucu yağların nematisidal etkileri Kök lezyon nematodu türüne bağlı olarak değişiklik göstermiş, P. neglectus türünde Coriondrum sativum L., P. thornei türünde Ferulago cassia Boiss, P. penetrans türünde ise Foeniculum vulgare Miller ve Anethum graveolens L. uçucu yağları diğer uygulamalardan daha yüksek etkinlik göstermiştir. Uçucu yağ uygulamalarına bağlı olarak gerçekleşen nematod ölüm oranları P. neglectus türünde % 24.3-64.7, P. penetrans türünde % 23.7-66.7 ve P. thornei türünde % 33.7-85.0 arasında bulunmuştur. Araştırmada, uçucu yağ aktif maddelerinden linalool, phallendrene ve 2,3,6 trimetilbenzaldehyde bileşenlerini içeren bitkilerin yüksek, anethole ve carvone bileşenlerini içeren bitkilerin orta, caratol ve curzerene bileşenlerini içerenlerin ise düşük nematoksik aktivite gösterdiği anlaşılmıştır. Apiaceae familyası türlerinin Pratylenchus türlerine karşı nematisidal etkilerinin yüksek fakat değişken olduğu, bu nedenle yüksek aktivite gösteren uçucu yağların farklı dozlar kullanılarak arazi koşullarında etkinliklerinin denenmesi ile daha kesin sonuçlara ulaşılabileceği anlaşılmıştır.

Anahtar Kelimeler:

Pratylenchus spp, Uçucu yağ, , Nematoksik etki, Apiaceae

Nematicidal Activity of Essential Oils from Apiaceae Family Plants of Turkey Against Root Lesion Nematodes

In our study, the nematicidal activity of 12 essential oils from Apiaceae family plants of Turkey against Pratylenchus penetrans, P. thornei and P. neglectus root lesion nematodes were investigated. The study was conducted in vitro conditions and concentration of 1000 ppm of each essential oil was used. The nematicidal effect was found to depending on root lesion nematode species and high nematicidal effect were found P. neglectus species; Coriondrum sativum L., P. thornei species; Ferulago cassia Boiss and P. penetrans Foeniculum vulgare Miller and Anethum graveolens L. Mortality rates depending on essential oil applications varied between 24.3-64.7% in P. neglectus species, 23.7-66.7% in P. penetrans and 33.7-85.0% in P. thornei. In this study, the nematicidal activity were found high that essential oil active ingredients of plants containing linalool, phallendrene and 2,3,6 trimethylbenzaldehyde components while the plants containing anethole and carvone components were medium nematicidal activity and containing caratol and curzerene components showed low. It has been understood that the nematicidal activity of Apiaceae family species against Pratylenchus species are high but variable, therefore, more definite results can be achieved by testing the effectiveness of highly active essential oils using different doses in field conditions.

Keywords:

Apiaceae, Nematicidal effect, Pratylenchus spp., Essential oil., Essential oil,

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Andres M., Gonzalez-Coloma A., Sanz J., Burillo J., & Sainz P. (2012). Nematicidal activity of essential oils: a review Phytochemistry Review DOI 10.1007/s11101-012-9263-3.
Avato, P., Laquale, S., Argentieri, M. P., Lamiri, A., Radicci, V., & D’Addabbo, T. (2017). Nematicidal activity of essential oilsfrom aromatic plants of Morocco. Journal of pest science, 90(2), 711-722.
Barbosa P., Lima A. S., Vieira P., Dias L. S., Tinoco M. T., & Barroso J. G. (2010). Nematicidal activity of EOs and volatiles derived from portuguese aromatic flora against the pinewood nematode, Bursaphelenchus xylophilus. J Nematol 42:8–16
Bauske E. M., Rodr´ıguez-Ka´bana R., Estau´n V.,Kloepper J. W., & Robertson D. G. (1994). Management of Meloidogyne incognita on cotton by use of botanical aromatic compounds. Nematropica, 24:143–50.
Blair, B. L. & Stirling, G. R. (2007). The role of plant-parasitic nematodes in reducing yield of sugarcane in fine-textured soils in Queensland, Australia. Australian Journal of Experimental Agriculture, 47(5), 620-634.
Calvet C., Pinochet J., Camprubi A., Estaun V., & Rodriquez-kabana R. (2001). Evaluation of natural chemical compounds against root lesion and root knot nematodes and side effects on the infectivity of arbuscular mycorrhizal fungi. European Journal of Plant Pathology, 107, 601-605.
Castillo P., & Vovlas N. (2007). Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management. Nematology Monographs and Perspectives Volume 6.
Cavanagh, H., & M., A. (2007). Antifungal activity of the volatile phase of essential oils: A brief review, Nat. Prod. Commun, 2, 1297–1302.
Dorman, H., J., D., & Deans, S.G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils, Journal of Applied Microbiology, .88, 308-316.
Duschatzky, C. B., Martinez, A. N., Almeida, N. V., & Bonivardo, S. L. (2004). Nematicidal activity of the essential oils of several Argentina plants against the root-knot nematode. Journal of Essential Oil Research, 16(6), 626-628.
El-Habashy, D. E., Abdel Rasoul, M. A., & Abdelgaleil, S. A. M. (2020). Nematicidal activity of phytochemicals and their potential use for the control of Meloidogyne javanica infected eggplant in the greenhouse. European Journal of Plant Pathology. doi:10.1007/s10658-020-02079-6
Figueiredo, A. C., Barroso, J. G., Pedro, L. G., & Scheffer, J. J. (2008). Factors affecting secondary metabolite production inplants: volatile components and essential oils. Flavour and Fragrance journal, 23(4), 213-226.
Gupta A., Sharma S., & Naik S.N. (2011). Biopesticidal value of selected EOs against pathogenic fungus, termites, and nematodes. International Biodeterioration Biodegradation, 65:703–707.
Hafez, S. L., Al-Rehıayanı, S., Thornton, M. & Sundararaj, P. (1999). Differentiation of two geographically isolated populations of Pratylenchus neglectus based ontheir parasitism of potato and interactionwithVerticilliumdahliae. Nematropica 29, 25-36.
Jones, M. G. K. & Fosu-Nyarko, J. (2014). Molecular biology of root lesion nematodes (Pratylenchus spp.) and their interaction with host plants. Annals of applied biology,164(2), 163-181. https: //doi.org/10.1111/aab.12105
Kim, S. S., Baik, J. S., Oh, T. H., Yoon, W. J., Lee, N. H., & Hyun. C. G. (2008). Biological activities of Korean Citrus obovoides and Citrus natsudaidai essential oils against acne-inducing bacteria. Biosci Biotechnol Biochem 72: 2507–2513
Kong J., Lee S., Moon Y., Lee S., & Ahn Y. (2006). Nematicidal activity of plant EOs against Bursaphelenchus xylophilus (Nematoda: aphelenchoididae). Journal of Asia Pacific Entomology, 9:173–178.
Lang, G., & Buchbauer, G. (2012). A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals, Flavour Fragr. J., 27, 13–39. Lee H. S., (2006). Mosquito larvicidal activity of aromatic medicinal plant oils against Aedes aegypti and Culex pipiens pallens. Journal of the American Mosquito Control Association, 22: 292-295.
Liang, J. Y., Ning, A. Q., Lu, P. Y., Shao, Y. Z., Xu, J., Yang, Y. Y., & Wang, H. L. (2020). Chemical Composition and BiologicalActivity of Essential Oil Extracted from the Aerial Part of Elsholtzia fruticosa against Ditylenchus destructor. Journal of Essential Oil Bearing Plants, 23(3), 575-582.
Marotti M., & Piccaglia R. (1992). The Influence of Distillation Conditions on the Essential Oil Composition of Three Varieties of Foeniculum vulgare Mill. Journal of Essential Oil Research, 4: 569-576.
Mudiope, J., Adipala, E., Coyne, D., & Sikora, R. (2004). Monoxenic culture of Pratylenchus sudanensis on carrot disks, with evidence of differences in reproductive rates between geographical isolates. Nematology, 6(4), 617-619.
Ntalli N. G., Ferrari F., Giannakou I., & Menkissoglu-Spiroudi U. (2011). Synergistic and antagonistic interactions of terpenes against Meloidogyne incognita and the nematicidal activity of EOs from seven plants indigenous to Greece. Pest Management Science 67:341–351.
Ntalli, N., G., Ferrari, F., Giannakou, I., & Menkissoglu-Spiroudi, U. (2010). Phytochemistry and nematicidal activity of the essential oils from 8 Greek Lamiaceae aromatic plants and 13 terpene components, J. Agric. Food Chem., 58, 7856–7863.
Oka Y., Nacar S., Putievsky E., Ravid U., Yaniv Z., & Spiegel Y. (2000). Nematicidal activity of EOs and their components against the root-knot nematode. Phytopathology, 90:710–715.
Osman A.A., & Viglierchio D.R. (1988). Efﬁcacy of biologically active agents as non traditional nematicides for Meloidogyne javanica. Review of N´ematology,11:93–98.
Payan, L. A., & Dickson, D. W. (1990). Comparison of populations of Pratylenchus brachyurus based on isozyme phenotypes. Journal of Nematology, 22(4), 538.
Pinochet, P., Cenis, J. L., Fernandez, C., Doucet, M., & Maruli, J. (1994). Reproductive fitness and random amplified polymorphic DNA variation among isolates of Pratylenchus vulnus. Journal of Nematology, 26(3), 271.
Renco M., & Balezentiene L. (2015). An analysis of soil free-living and plant-parasitic nematode communities in three habitats invaded by Heracleum sosnowskyi in central. Biol Invasions, 17:1025–1039.
Saad, M. M. G., Abou-Taleb, H. K., & Abdelgaleil, S. A. M. (2018). Insecticidal activities of monoterpenes and phenylpropenes against Sitophilus oryzae and their inhibitory effects on acetylcholinesterase and adenosine Eur J Plant Pathol triphosphatases. Applied Entomology and Zoology, 53(2), 173–181.
Siddiqui A., & Zaki M. J. (2017). Effıcacy of Some Seeds of Famıly Apıaceae agaınst Root Knot Nematode, Meloıdogyne javanıca (Treub) Chıtwood. Internatıonal Journal of Bıology and Bıotechnology, 14 (1): 89-94.
Smiley R.W., & Nicol J. M., (2009). Nematodes which challenge global wheat production. In: Carver B.F. (ed.) Wheat science and trade, Wiley-Blackwell, pp. 171–187.
Söğüt M. A., & Devran Z. (2011). Distribution and molecular identification of root lesion nematodes in temperate fruit orchards of Turkey. Nematropica, 41: 91-99.
Stein S. E., 1990. National Institute of Standardsand Technology (NIST) Mass Spectral Database and Software, Version 3.02, Juen USA.
Tsao R., & Yu Q. (2000). Nematicidal Activity of Monoterpenoid Compounds against Economically Important Nematodes in Agriculture. Journal of Essential Oil Research, 12:3, 350-354.
Viglierchio D.R., Wu F.F., 1989. Selected biological inhibitors for Heterodera schachtii control. Nematropica, 19:75–79.
Zuckerman B. M., Mai W .F., & Harrison M. B. (1985). Plant Nematology Laboratory Manual. The University of Massachusetts Agricultural Experiment Station Amherst, Massachusetts 01003, Pp: 212