Object: One of the most frequently isolated microorga- nisms urinary tract infections (UTI) is E. coli. In this study, we aimed to determine susceptibility of E. coli strains isolated from patients with UTI against nitrofurantoin, fosfomycin trometamol and some other frequently used antibiotics. Material and Method: Totally 140 E. coli strains were used in the study. Extended-spectrum beta-lactamase (ESBL) test was performed by double-disc synergy test and antibiotic susceptibility was determined by Kirby- Bauer disc diffusion method according to Clinical and Laboratory Standards Institute (CLSI) criteria. Results: It was found that 93.6% of the isolates were susceptible to nitrofurantoin whereas 91.4% of the isolates were susceptible to fosfomycin. Among the other antibiotics, susceptibility was highest to cefepime and ceftazidime and lowest to ampicillin. Regarding ESBL production of the isolates, there was no significant difference between hospital and community acquired infections, and fosfomycin and nitrofurantoin resistance. Conclusion: It was concluded that nitrofurantoin and fosfomycin could be preferred in the empiric treatment of UTI