Investigation of chemopreventif properties of Urtica Dioica L.,in MCF-7 and MDA 231 breast cancer cell lines

Giriş: Gelişmekte olan dünyanın en büyük sağlık prob-lemi tedavide yetersiz kalınan kanser hastalığıdır. Çalış-manın amacı Türkiyede yetişen ısırgan otunun var olabilecek kanser oluşumunu engelleyici özelliklerinin ve etki mekanizmalarının araştırılmasıdır. Materyal ve Metot: Isırgan sulu ve asit bitki özütlerinde, antioksidan aktivite tayin metotlarından toplam fenol, flavon ve serbest radikal süpürücü aktivitesi çalışılmıştır. U. dioica özütünün, 48 saat süreyle, artan konsantras-yonlarda MCF7 ve MDA-231 hücre kültürleri üzerindeki sitotoksik etkisi XTT yöntemi ile tespit edildi. Sonuç: Çalışmada kullanım gören hücre kültürü, fenol flavon tayini ve HPLC sonuçlarına göre araştırma bulgu-ları: ısırgan bitkisinin etki mekanizmalarıyla kanser oluşumunu engelleyici özelliğini göstermektedir.

MCF-7 ve MDA 231 hücre hatlarında Urtica Dioica'nın kemopreventif özelliklerinin araştırılması

Introduction: Cancer is a major health problem in developing world with mostly unsufficient treatment. The interest of present research is to check traditional Turkish herb Urtica diocia L which seems to provide promising agents with the potential protection against cancer and search for their mechanism of action. Material and Method: Total phenol and flavon contents and radical scavenging activity (RSA) were also determined to survey antioxidant potential of U. diocia aqueous and acid extracts. Cells were incubated with the extract in the variying concentration range for 48 h and the cytotoxicity was determined using the XTT assay. Conclusion: According to the results of determination of free radical scavenging activity and cytotoxicity assay, HPLC-DAD profiles of U.dioica which seems to provide promising agents with the potential protection against cancer and findings from its mechanism of action.

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