Mustafa ÖZTOP, Kenan ÇINAR, Emel DEMİRBAĞ

Keklik ve Bıldırcın Konjunktiva Epitelindeki Glikokonjugatların Lektin Histokimyası

Bu çalışmada keklik (Alectoris chukar) ve bıldırcın (Coturnix coturnix) konjonktiva epitelindeki glikokonjugatların dağılımının belirlenmesi amaçlandı. Her iki türden alınan gözkapağı örnekleri rutin doku takibi işlemlerinden geçildi. Glikokonjugat ekspresyonunu göstermek için alınan kesitlere lektin histokimyası uygulandı. Bu amaçla altı farklı HRP-bağlı lektin (Con A, UEA-I, PNA, HPA, MAA ve BSA I-B4) kullanıldı. Hem keklikte hem de bıldırcında konjunktiva epiteli, goblet hücreleri ve goblet olmayan hücrelerden oluşmaktadır. Hem PNA hem de HPA değişen derecelerde bıldırcın ve keklik konjunktival goblet hücreleri ve goblet olmayan hücrelere bağlanmıştır. Con A her iki türde goblet olmayan hücreler ile reaksiyon vermiştir. Keklik konjunktival goblet hücreleri Con A’ya karşı herhangi bir reaksiyon göstermezken; bıldırcın konjunktival goblet hücreleri Con A’ya karşı çok zayıf reaksiyon göstermiştir. UEA-I, keklikte herhangi bir hücre tipine karşı reaksiyon göstermezken; bıldırcın konjunktival goblet hücreleri ve goblet olmayan hücreler UEA-I ile reaksiyon vermiştir. Her iki türde konjunktival epitelyal yüzeyler Con A, UEA-I, PNA ve HPA ile reaksiyon vermiştir. Bununla birlikte, MAA ve BSA I-B4 her iki türde herhangi bir hücre ve/veya kısma bağlanmamıştır. Bu sonuçlar glikokonjugat komposizyonunun bu türlerin goblet ve goblet olmayan hücrelerinde farklı olabildiğini, ancak goblet ve goblet olmayan hücrelerin içeriklerinin salındığı konjunktival epitelyal yüzeylerde çok benzer olabildiğini göstermiştir.

Anahtar Kelimeler:

Keklik, Bıldırcın, Lektin Histokimyası, Glikokonjugat, Konjunktiva, Goblet hücresi

Lectin Histochemistry of the Glycoconjugates in Partridge and Quail Conjunctival Epithelia

The aim of this study was to examine the distribution of the glycoconjugates in conjunctival epithelium of partridge (Alectoris chukar) and quail (Coturnix coturnix). Eyelid samples harvested from both species were subjected to routine tissue processing. Lectin histochemistry was applied to sections in order to demonstrate the expression of glycoconjugates. Six different HRP-conjugated lectins (Con A, UEA-I, PNA, HPA, MAA and BSA I-B4) were used for this purpose. The conjunctival epithelium in both partridge and quail consisted of goblet cells and nongoblet cells. Both PNA and HPA bound, to varying degrees, to the goblet cells and nongoblet cells in conjunctival epithelium of partridge and quail. Con A reacted with nongoblet cells in both species. While partridge conjunctival goblet cells showed no reaction to Con A, quail conjunctival goblet cells had a very weak reaction to Con A. UEA-I did not bind to any cells in partridge, however, quail conjunctival goblet cells and nongoblet cells could react to UEA-I. In both species, conjunctival epithelial surfaces reacted with Con A, UEA-I, PNA and HPA. However, MAA and BSA I-B4 did not bind any cell and/or part in both partridge and quail conjunctiva. The present data suggest that composition of glycoconjugates could be different between goblet cells and nongoblet cells of these species, but it could be very similar at conjunctival epithelial surfaces on which the content of the goblet cells and nongoblet cells is released.

Keywords:

Partridge, Quail, Glycoconjugates, Conjunctiva, Lectin histochemistry, Goblet Cell,

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