Şinasi BAYRAM, Onur ERSOY, Ayşegül ÇERKEZKAYABEKİR, Melike SAPMAZ, Turan KARACA, Gülnur KIZILAY ÖZFİDAN

Fukoidanın Diyabetik Sıçan Testis Dokularındaki PCNA, INSL3, JNK, TGF-β1, IL-1β ve Akt İfadelerine Etkisi

Çalışmamızın amacı, diyabetik testis dokularında gözlenen hasarlarda etkili olabilme potansiyeli yüksek bir antioksidan olan fukoidanın, spermatogenez seri hücrelerine proliferasyon, apoptozis ve inflamasyon yönünden olası etkilerini araştırmaktır. Fukoidan; çeşitli amaçlarla tablet formunda tüketici kullanımına sunulmuş olmasına rağmen, diyabetli erkek hastalarda infertiliteye yönelik bir değerlendirme yapabilmek için yeterli veriler bulunmamaktadır. Bu amaçla, testis dokusunda prolifere hücre nükleer antijeni (PCNA), insulin-benzeri peptid 3 (INSL3), fosfo (f)-c-Jun N-terminal kinaz (f-JNK), dönüştürücü büyüme faktörü-β1 (TGF-β1), fosfo-serin/treonin protein kinaz (f-Akt) ve interlökin-1β (IL-1β) gibi biyobelirteçlerin ifadelerinin değerlendirilmesi planlanmıştır. Çalışmamızda yirmi dört adet Wistar albino erkek sıçan kullanılarak 4 deney grubu (n=6); K: Kontrol grubu, D: diyabet grubu; 40 mg/kg streptozotosin (STZ, 5 ardışık gün, intraperitoneal (i.p.) verilen grup, EF grubu: 40 mg/kg STZ i.p (5 ardışık gün) + 50 mg/kg i.p. fukoidan (Diyabet oluşumunun ertesi günü başlanarak 6 hafta süresince, günaşırı bir kez) verilen grup, GF grubu: 40 mg/kg STZ i.p. (5 ardışık gün) + 50 mg/kg i.p. fukoidan (diyabet oluşturulduktan 15 gün sonra, 6 hafta süresince günaşırı bir kez) verilen grup oluşturulmuştur. Diyabet indüksiyonuyla; immatur hücrelerde dökülme ve seminifer tübül duvarındaki hücrelerde izlenen sitoplazma kayıpları, belirgin dejeneratif değişiklikler olarak görülmüştür. Bununla birlikte diyabet, proliferasyon indeksinin yanısıra INSL3 ve f-Akt immunoreaktivitelerinde azalmaya; f-JNK, TGF-β1, IL-1β immunreaktivitelerinde ise artışa neden olmuştur. EF ve GF gruplarında ise çalışılan tüm parametrelerde, diyabetin etkilerini azaltma yönünde etki gözlenmiştir. Yaptığımız çalışma sonucunda, moleküler düzeyde etkili olabildiği önceki çalışmalarla da saptanan fukoidanın; diyabetik testis dokusundaki histopatolojik hasarlara karşı koruyucu etkileriyle, infertilite gibi üreme sağlığında oluşabilecek ciddi problemlerin önlenmesine katkı yapacağı kanısındayız.

Anahtar Kelimeler:

Fukoidan, Testis, Diyabet, JNK, INSL3

Effect of Fucoidan on PCNA, INSL3, JNK, TGF-β1, IL-1β and Akt Expressions in Diabetic Rat Testicular Tissues

The aim of our study is to evaluate the possible effects of fucoidan, which is an antioxidant with high potential to be effective in histopathological damages in diabetic testicular tissue, on the proliferation, Leydig cell function, apoptosis and inflammation. For this purpose, we planned to evaluate protein expression of proliferated cell nuclear antigen (PCNA), insulin-like peptide 3 (INSL3) phospho-c-Jun N-terminal kinase (p-JNK), transforming growth factor-β1 (TGF-β1), interleukin-1β (IL-1β) phospho-serine/threonine protein kinase (p-Akt) in testicular tissue. Although fucoidan has been presented to consumer use in tablet form for various purposes, there are insufficient data to make an assessment of infertility in male patients with diabetes. The animals were divided into four groups (n=6) using twenty-four Wistar albino male rats; K: control group, Group D: 40 mg/kg streptozotocin (STZ, 5 consecutive days, intraperitoneal (i.p.), EF group: 40 mg/kg STZ i.p (5 consecutive days)+50 mg/kg i.p. fucoidan (one time, every other day, during six weeks), GF group: 40 mg/kg STZ (5 consecutive days)+50 mg/kg i.p. fucoidan (after fifteen days induction diabetes, every other day, during six weeks). The shedding of immature cells and loss of the seminiferous tubule wall were seen as significant degenerative changes by diabetes induction. However diabetes decreased proliferation index, INSL3 and f-Akt immunoreactivity, whereas increased f-JNK, TGF-β1, IL-1β immunoreactivity. All parameters studied in EF and GF groups was observed reducing effects of diabetes. It was concluded that fucoidan may contribute to the prevention of some serious problems that may occur in male reproductive health, such as infertility.

Keywords:

Fucoidan, Testes, Diabetes, JNK, INSL3,

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