ÜLKEMİZDE GÜNEY MARMARA BÖLGESİ’NDEKİ BAL ARILARINDA NOSEMA CERANAE’NİN MİKROSKOBİK VE MOLEKÜLER OLARAK BELİRLENMESİ

Bu çalışmada Bursa ve Balıkesir çevresindeki kolonilerde mikroskobik olarak Nosema türlerinin varlığını belirlemek ve multipleks PCR yöntemi ile kolonilerin hangi Nosema türü (N. ceranae ve N. apis) ile enfekte olduğunu tespit etmek amaçlanmıştır. Mikroskobik inceleme sonucunda, 14 koloniden 11 tanesindeki (%78,6) bireylerde Nosema sporları bulunmuş ve taranan bireylerin Nosema ile enfekte olduğu tespit edilmiştir. Bu kolonilerden alınan örneklerde multipleks PCR metodu kullanılarak moleküler tanımlama ile N. ceranae’nın 16S rRNA gen bölgesini karakterize eden bantlar bulunmuştur. Çalışmada kolonilerin büyük çoğunluğunun N. ceranae ile enfekte olduğu tespit edilmiştir.

Anahtar Kelimeler:

Apis mellifera, Nosema apis, Nosema ceranae, tanımlama, Bursa ve Balıkesir

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