Mohamed Badreddine MOKHTARI, Ibtissem EL OUAR, Ibtissem ZEGHINA, Maya Abir TARTOUGA, Asma GHORAB, Laid BAHRI, Chawki BENSOUICI

Cezayir'in Kuzeydoğu Bölgesinden Toplanan Bazı Arı Polenlerinin Palinolojik Analizi, Fenolik Bileşenleri ve Anti-İnflamatuar Aktivitesi

Arı poleni, çiçek poleni ve nektar ile arı tükürük maddelerinin karmaşık bir karışımıdır. Karbonhidratlar, proteinler, lipidler, vitaminler ve fenolik bileşikler gibi birçok biyolojik olarak aktif maddenin zengin bir kaynağı olarak ünlüdür. Bileşimi, botanik kökenlere ve edafoklimatik koşullara göre büyük ölçüde değişir. Bu çalışmada, Cezayir'in kuzeydoğusunda bulunan farklı arılıklardan toplanan insan tüketimine yönelik sekiz arı poleninin botanik orijini, toplam fenolik içerikleri, toplam flavonoid içerikleri, toplam flavonol içerikleri ve in vivo antiinflamatuar aktiviteleri belirlendi ve karşılaştırıldı. Tüm örnekler, 22 botanik familyaya ait kırk polen türünün tanımlanmasıyla heterofloral olarak saptandı. Toplam fenolik içerik 752,94 ± 17,78 ile 12247,06 ± 40,04 mg GAE/ 100 arasında değişirken, toplam flavonoid içeriği 2680,55 ± 12,02 ila 8506,94 ± 15,56 mg QE/ 100g arasında değişmekte ve toplam flavonol içeriği 4978,87 ± 33,39 ile arasında değişmektedir. 7903,75 ± 24,39 mg QE/ 100g. Sonuçlar etanolik ekstraktların büyük kısmının iyi bir anti-inflamatuar aktiviteye sahip olduğunu göstermektedir. Sonuç olarak, yukarıda bahsedilen tüm heterofloral arı poleni örnekleri, potansiyel bir anti-inflamatuar aktiviteye sahip zengin bir polifenol kaynağı olabilir.

Anahtar Kelimeler:

arı poleni, palinolojik analiz, fenolik içerik, antiinflamatuar aktivite

PALYNOLOGICAL ANALYSIS, PHENOLIC COMPONENTS AND ANTI-INFLAMMATORY ACTIVITY OF SOME BEE POLLENS COLLECTED FROM THE NORTHEAST REGION OF ALGERIA

Bee pollen is a complex blend of flower pollen and nectar with bee salivary substances. It is famously known as a rich source of many biologically active substances such as: carbohydrates, proteins, lipids, vitamins and phenolic compounds. Its composition varies greatly according to the botanical origins and edaphoclimatic conditions. In this work, the botanical origin, total phenolic contents, total flavonoid contents, total flavonol contents and the anti-inflammatory activity in vivo of eight bee pollens intended for human consumption which were collected from different apiaries located in the northeast of Algeria, were determined and compared. All samples were heterofloral with the identification of forty pollen types belonging to 22 botanical families. Total phenolic contents varied between 752.94 ± 17.78 and 12247.06 ± 40.04 mg GAE/ 100, while the total flavonoid contents ranged from 2680.55 ± 12.02 to 8506.94 ± 15.56 mg QE/ 100g, and the total flavonol contents were in the interval between 4978.87 ± 33.39 and 7903.75 ± 24.39 mg QE/ 100g. Results show that the bulk of the ethanolic extracts had a good anti-inflammatory activity. As a conclusion, all the aforementioned heterofloral bee pollen samples can be a rich source of polyphenols with a potential anti-inflammatory activity.

Keywords:

bee pollen, palynological analysis, phenolic contents, anti-inflammatory activity,

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Araújo, J., Chambó, E., Costa, M., Cavalcante da Silva, S., Lopes de Carvalho, C., & M. Estevinho, L. (2017). Chemical Composition and Biological Activities of Mono- and Heterofloral Bee Pollen of Different Geographical Origins. International Journal of Molecular Sciences, 18(5), 921. https://doi.org/10.3390/ijms18050921

Arzi, A., Olapour, S., Yaghooti, H., & Sistani Karampour, N. (2015). Effect of Royal Jelly on Formalin Induced-Inflammation in Rat Hind Paw. Jundishapur Journal of Natural Pharmaceutical Products, 10(1), 8–11. https://doi.org/10.17795/jjnpp-22466

Asmae, E. G., Nawal, E. M., Bakour, M., & Lyoussi, B. (2021). Moroccan Monofloral Bee Pollen: Botanical Origin, Physicochemical Characterization, and Antioxidant Activities. Journal of Food Quality, 2021. https://doi.org/10.1155/2021/8877266

Aylanc, V., Tomás, A., Russo-Almeida, P., Falcão, S. I., & Vilas-Boas, M. (2021). Assessment of bioactive compounds under simulated gastrointestinal digestion of bee pollen and bee bread: Bioaccessibility and antioxidant activity. Antioxidants, 10(5). https://doi.org/10.3390/antiox10050651

Bogdanov, S. (2004). Quality and Standards of Pollen and Beeswax. APIACTA, 38, 334–341.

Damas, J., Liégeois, J. F., Damas, J., & Liégeois, J.-F. (1999). The inflammatory reaction induced by formalin in the rat paw. In Naunyn-Schmiedeberg’s Arch Pharmacol (Vol. 359).

Daoud, A., Malika, D., Bakari, S., Hfaiedh, N., Mnafgui, K., Kadri, A., & Gharsallah, N. (2019). Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of Date Palm Pollen (DPP) from two Tunisian cultivars. Arabian Journal of Chemistry, 12(8), 3075–3086. https://doi.org/10.1016/j.arabjc.2015.07.014

de França Alves, R., & de Assis Ribeiro DosSantos, F. (2014). Plant sources for bee pollen load production in Sergipe, northeast Brazil. Palynology, 38(1), 90–100. https://doi.org/10.1080/01916122.2013.846280

Eraslan, G., Kanbur, M., Silici, S., Cem Liman, B., Altinordulu, Ş., & Soyer Sarica, Z. (2009). Evaluation of protective effect of bee pollen against propoxur toxicity in rat. Ecotoxicology and Environmental Safety, 72(3), 931–937. https://doi.org/10.1016/j.ecoenv.2008.06.008

Estevinho, L. M., Rodrigues, S., Pereira, A. P., & Feás, X. (2012). Portuguese bee pollen: Palynological study, nutritional and microbiological evaluation. International Journal of Food Science and Technology, 47(2), 429–435. https://doi.org/10.1111/j.1365-2621.2011.02859.x

Fatrcová-Šramková, K., Nôžková, J., Kačániová, M., Máriássyová, M., Rovná, K., & Stričík, M. (2013). Antioxidant and antimicrobial properties of monofloral bee pollen. Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes, 48(2), 133–138. https://doi.org/10.1080/03601234.2013.727664

Fereidoni, M., Ahmadiani, A., Semnanian, S., & Javan, M. (2000). An accurate and simple method for measurement of paw edema. Journal of Pharmacological and Toxicological Methods, 43(1), 11–14. https://doi.org/10.1016/S1056-8719(00)00089-7

Gan, T. J. (2010). Diclofenac: An update on its mechanism of action and safety profile. In Current Medical Research and Opinion (Vol. 26, Issue 7, pp. 1715–1731). Informa Healthcare. https://doi.org/10.1185/03007995.2010.486301

Gercek, Y. C., Celik, S., & Bayram, S. (2021). Screening of Plant Pollen Sources, Polyphenolic Compounds, Fatty Acids and Antioxidant/Antimicrobial Activity from Bee Pollen. Molecules, 27(1), 117. https://doi.org/10.3390/molecules27010117

Ghorab, A., Mesbah, M., Nakib, R., Kabli, N., Bekdouche, F., & Seijo, M. C. (2021). Input to the knowledge of the melliferous plants diversity in Babors Kabylia’s region (North-East of Algeria). Livestock Research for Rural Development, 33. www.lrrd.org/lrrd33/10/33119asma.html

Ghorab, A., Rodríguez-Flores, M. S., Nakib, R., Escuredo, O., Haderbache, L., Bekdouche, F., & Seijo, M. C. (2021). Sensorial, melissopalynological and physico-chemical characteristics of honey from Babors Kabylia’s region (Algeria). Foods, 10(2). https://doi.org/10.3390/foods10020225

Khalifa, S. A. M., Elashal, M. H., Yosri, N., Du, M., Musharraf, S. G., Nahar, L., Sarker, S. D., Guo, Z., Cao, W., Zou, X., Abd El-Wahed, A. A., Xiao, J., Omar, H. A., Hegazy, M. E. F., & El-Seedi, H. R. (2021). Bee pollen: Current status and therapeutic potential. In Nutrients (Vol. 13, Issue 6). MDPI AG. https://doi.org/10.3390/nu13061876

Komosinska-vassev, K., Olczyk, P., Ka, J., Mencner, L., & Olczyk, K. (2015). Bee Pollen : Chemical Composition and Therapeutic Application. 2015. https://doi.org/http://dx.doi.org/10.1155/2015/297425

Kumaran, A., & Joel Karunakaran, R. (2007). In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT - Food Science and Technology, 40(2), 344–352. https://doi.org/10.1016/j.lwt.2005.09.011

LeBlanc, B. W., Davis, O. K., Boue, S., DeLucca, A., & Deeby, T. (2009). Antioxidant activity of Sonoran Desert bee pollen. Food Chemistry, 115(4), 1299–1305. https://doi.org/10.1016/j.foodchem.2009.01.055

Lopes, A. J. O., Vasconcelos, C. C., Garcia, J. B. S., Dória Pinheiro, M. S., Pereira, F. A. N., Camelo, D. D. S., de Morais, S. V., Freitas, J. R. B., da Rocha, C. Q., de Sousa Ribeiro, M. N., & do Socorro de Sousa Cartágenes, M. (2020). Anti‐inflammatory and antioxidant activity of pollen extract collected by Scaptotrigona affinis postica: In silico, in vitro, and in vivo studies. Antioxidants, 9(2), 1–16. https://doi.org/10.3390/antiox9020103

Lopes, A. J. O., Vasconcelos, C. C., Pereira, F. A. N., Silva, R. H. M., Queiroz, P. F. D. S. Q., Fernandes, C. V., Garcia, J. B. S., Ramos, R. M., da Rocha, C. Q., Lima, S. T. D. J. R. M., Cartágenes, M. D. S. D. S., & de Sousa Ribeiro, M. N. (2019). Anti-Inflammatory and antinociceptive activity of pollen extract collected by stingless bee Melipona fasciculata. International Journal of Molecular Sciences, 20(18). https://doi.org/10.3390/ijms20184512

Louveaux, J., Maurizio, A., & Vorwohl, G. (1978). Methods of Melissopalynology. Bee World, 59(4), 139–157. https://doi.org/10.1080/0005772X.1978.11097714

Maruyama, H., Sakamoto, T., Araki, Y., & Hara, H. (2010). Anti-inflammatory effect of bee pollen ethanol extract from Cistus sp. of Spanish on carrageenan-induced rat hind paw edema. In BMC Complementary and Alternative Medicine (Vol. 10). http://www.biomedcentral.com/1472-6882/10/30

Nogueira, C., Iglesias, A., Feás, X., & Estevinho, L. M. (2012). Commercial bee pollen with different geographical origins: A comprehensive approach. International Journal of Molecular Sciences, 13(9), 11173–11187. https://doi.org/10.3390/ijms130911173

Saadia Tamali, H., & Özkırım, A. (2019). Beekeeping Activities in Turkey and Algeria. In Mellifera (Vol. 19, Issue 1).

Şahin, S., & Karkar, B. (2019a). The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases. Journal of Food Biochemistry, 43(7), 1–8. https://doi.org/10.1111/jfbc.12888

Şahin, S., & Karkar, B. (2019b). The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases. Journal of Food Biochemistry, 43(7). https://doi.org/10.1111/jfbc.12888

Sattler, J. A. G., de Melo, I. L. P., Granato, D., Araújo, E., da Silva de Freitas, A., Barth, O. M., Sattler, A., & de Almeida-Muradian, L. B. (2015). Impact of origin on bioactive compounds and nutritional composition of bee pollen from southern Brazil: A screening study. Food Research International, 77, 82–91. https://doi.org/10.1016/j.foodres.2015.09.013

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16(3), 144 LP – 158.

Thakur, M., & Nanda, V. (2020). Composition and functionality of bee pollen: A review. Trends in Food Science and Technology, 98, 82–106. https://doi.org/10.1016/j.tifs.2020.02.001

Topçu, G., Ay, M., Bilici, A., Sarikürkcü, C., Öztürk, M., & Ulubelen, A. (2007). A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chemistry, 103(3), 816–822. https://doi.org/10.1016/j.foodchem.2006.09.028

TUTUN, H., KAYA, M. M., USLUER, M. S., & KAHRAMAN, H. A. (2021). Bee pollen: Its antioxidant activity. Uludağ Arıcılık Dergisi, 21(1), 8–20. https://doi.org/10.31467/uluaricilik.896045

Yildiz, O., Can, Z., Saral, Ö., Yuluǧ, E., Öztürk, F., Aliyazicioǧlu, R., Canpolat, S., & Kolayli, S. (2013). Hepatoprotective potential of chestnut bee pollen on carbon tetrachloride-induced hepatic damages in rats. Evidence-Based Complementary and Alternative Medicine, 2013. https://doi.org/10.1155/2013/461478

Zerrouk, S., Seijo, M. C., Boughediri, L., Escuredo, O., & Rodríguez-Flores, M. S. (2014). Palynological characterisation of Algerian honeys according to their geographical and botanical origin. Grana, 53(2), 147–158. https://doi.org/10.1080/00173134.2014.897751

Žilić, S., Vančetović, J., Janković, M., & Maksimović, V. (2014). Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen. Journal of Functional Foods, 10, 65–74. https://doi.org/10.1016/j.jff.2014.05.007