Synthesis and Characterization of Veronica beccabunga Green Synthesized Silver Nanoparticles for The Antioxidant and Antimicrobial Activity

The use of nano-agents in the healthcare field is an important alternative in reducing the increasing pathogen resistance. One of the main purposes of the nano mechanism is that the secondary metabolites produced naturally in plants become more effective through metals. Silver (Ag) nanoparticles were obtained by green synthesis using Veronica beccabunga plant extract and AgNO3 in our study. Scanning electron microscopy was used for SEM, SEM / EDX images of synthesized silver nanoparticles. The interaction of V. beccabunga with Ag was explained by X-ray diffraction (XRD) analysis. The characterization process was performed using an ultraviolet-visible (UV-vis) spectrophotometer and Fourier converted infrared spectroscopy (FT-IR). It was determined that silver nanostructures have an important antioxidant potential as a result of 2,2-difenil-1-pikrilhidrazil (DPPH) analysis. The antimicrobial activity of synthesized metal nanoparticles was investigated, against some pathogens causing disease in humans, by the disk diffusion method. Ag NPs /Vb has been found to have antibacterial effects against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, Pseudomonas aeruginosa ATCC 27853 microorganisms. In our current study, Ag NPs / Vb appears to provide a remarkable effect for its use in medicine, pharmacology.

Anahtar Kelimeler:

Veronica beccabunga, Antimicrobial, Antioxidant, nanoparticle, characterization

Synthesis and Characterization of Veronica beccabunga Green Synthesized Silver Nanoparticles for The Antioxidant and Antimicrobial Activity

Keywords:

Veronica beccabunga, antimicrobial, antioxidant, nanoparticle, characterization,

PDF

___

Aazam, E.S., Zaheer, Z., 2016. Growth of Ag-nanoparticles in an aqueous solution and their antimicrobial activities against Gram-positive, Gram-negative bacterial strains and Candida fungus. Bioprocess and Biosystems Engineering, 39(4): 575-584.
Blois, M.S., 1958. Antioxidant determinations by the use of a stable free radical. Nature, 181(4617): 1199-1200.
Deng, J., Cheng, W., Yang, G., 2011. A novel antioxidant activity index (AAU) for natural products using the DPPH assay. Food Chemistry, 125(4): 1430-1435.
Dewick, P.M., 2001. Medicinal Natural Products. Biosynthetic Approach, New York: Wiley.
Djilas, S.M., Canadanovic-Brunet, J.M., Cetkovic, G.S., 2002. ESR spectroscopic investigation of antioxidant activity of Artemisia absinthium L. extracts. 6th International Conference on Applications of Magnetic Resonance in Food Science, September 18, Paris, France, pp. 40-45.
Fatemeh, K., Mohammad, J.M., Samaneh, K., 2017. The effect of silver nanoparticles on composite shear bond strength to dentin with different adhesion protocols. Journal of Applied Oral Science, 25(4): 367-373.
Haida, Z., Hakiman, M., 2019. A comprehensive review on the determination of enzymatic assay and nonenzymatic antioxidant activities. Food Science & Nutrition, 7(5): 1555-1563.
Ibraheem, I.B.M., Abd Elaziz, B.E.E., Saad, W.F., Fathy W.A., 2016. Green biosynthesis of silver nanoparticles using marine red algae Acanthophora specifera and its antimicrobial activity. Journal of Nanomedicine Nanotechnology, 7(6): 1-4.
Kedare, S.B., Singh, R.P., 2011. Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology, 48(4): 412-422.
Koçak, Y., Oto, G., Meydan, İ., Seçkin, H., 2020. Investigation of total flavonoid, DPPH radical scavenging, lipid peroxidation and antimicrobial activity of Allium schoenoprasum L. plant growing in Van Region. Yüzüncü Yıl University Journal of Agricultural Science, 30(1): 147-155. (In Turkish).
Mittal, A.K., Chisti, Y., Banerjee, U.C., 2013. Synthesis of metallic nanoparticlesusing plant extracts. Biotechnology Advances, 31: 346-356.
Mittal, J., Batra, A., Singh, A., Sharma, M.M., 2014. Phytofabrication ofnanoparticles through plant as nanofactories. Advances in Natural Sciences, 5(4): 1-10.
Nazar, R., Sangermano, M., Vitale, A., Bongiovanni, R., 2018. Silver polymer nanocomposites by photoreduction of AgNO3 and simultaneous photocrosslinking of the acrylic matrix: effect of PVP on Ag particle formation. Journal of Polymer Engineering, 38: 803-809.
Nikolova, M., 2011. Screening of radical scavenging activity and polyphenol content of Bulgarian plant species. Pharmacognosy Research, 3(4): 256-259.
Nóra, P., Nikolett, S., Rit, C., Monika, T., King, G., Tünde, D., Samuel, G.B., Erzsébet, V., Andrea, K., Tamás, K., 2019. Antioxidant potential of some plants used in folk medicine in Romania. Soc Stıınte Farmaceutıce Romanıa, 67(2): 323-330.
Patil, M.P., Kim, G.D., 2017. Eco-friendly approach for nanoparticles synthesis andmechanism behind antibacterial activity of silver and anticancer activity ofgold nanoparticles. Applied Microbiology and Biotechnology, 101: 79-92.
Selvam, K., Sudhakar, C., Govarthanan, M., Thiyagarajan, P., Sengottaiyan, A., Senthilkumar, B., Selvankumar, T., 2017. Eco-friendly biosynthesis and characterization of silver nanoparticles using Tinospora cordifolia (Thunb.) Miers and evaluate its antibacterial, antioxidant potential. Journal of Radiation Research and Applied Sciences, 10(1): 6-12.
Shah, M., Fawcett, D., Sharma, S., Tripathy, S., Poinern, G., 2015. Green synthesis of metallic nanoparticles via biological entities. Materials, 8(11): 7278-7308.
Sharma, O.P., Bhat, T.K., 2009. DPPH antioxidant assay revisited. Food Chemistry, 113(4): 1202-1205.
Singh, P., Kim,Y.J., Zhang, D., Chun-Yang, D., 2016. Biological synthesis of nanoparticles from plants and microorganisms. Trends in Biotechnology, 34(7): 588-599.
Tenover, F.C., 2006. Mechanisms of antimicrobial resistance in bacteria. The American Journal of Medicine, 119(6): 3-10.
Valsalam, S., Agastian, P., Esmail, G.A., Ghilan, A.K.M., Al-Dhabi, N.A., Arasu, M.V., 2019. Biosynthesis of silver and gold nanoparticles using Musa acuminata colla flower and its pharmaceutical activity against bacteria and anticancer efficacy. Journal of Photochemistry and Photobiology B: Biology, 201(4): 210-225.
Wang, L., Hu, C., Shao, L., 2017. The antimicrobial activity of nanoparticles: present situation and prospects for the future. International Journal of Nanomedicine, 12: 1227-1249.
Yaldız, G., Yüksek, T., Şekeroğlu, N., 2010. Medicinal and aromatic plants in flora of Rize province and their usage areas. 3rd National Black Sea Forestry Congress, 20-22 May, Artvin, Turkey, pp.1100-1114. (In Turkish).
Yaşar, S., Güler, G., Beram, A., Coşkun, D., Ozansoy, D., 2017. Volatile components of wormwood (Artemisia absinthium L.) leaves. The Journal of Graduate School of Natural and Applied Sciences of Mehmet Akif Ersoy University, 8(2): 148-152. (In Turkish).