Effects of Zinc Oxide on mRNA Expression of Genes in Brain Tissue of Rats

AIM: To investigate the expression of the DR4, DR5, OPG, DcR1, and DcR2 in rat brain tissue.MATERIAL and METHODS: Thirty rats were used in this study. The rats were divided into three groups as the control group (n=10),tumor group (n=10), and zincoxide (ZnO) nanoparticles (NP) treatment group (n=10). The reverse transcription polymerase chainreaction (RT-PCR) and Western Blotting methods were used to measure the expression of DR4, DR5, OPG, DcR1, DcR2 and β-actinin the brain tissues of all the three groups.RESULTS: Expression of DR4, DR5, OPG, DcR1, and DcR2 genes were decreased in the tumor group. Overexpression of DR4,DR5, OPG, DcR1, and DcR2 was observed in brain tissues of the ZnO-NP group.CONCLUSION: Increased expression of the DR4, DR5, OPG, DcR1, and DcR2 genes may play an important role in ZnO-NPtreatment of brain tumors.


1. Ahamed M, Akhtar MJ, Raja M, Ahmad I, Siddiqui MK, AlSalhi MS, Alrokayan SA: ZnO nanorod-induced apoptosis in human alveolar adenocarcinoma cells via p53, survivin and bax/bcl-2 pathways: Role of oxidative stress. Nanomedicine 7:904-913, 2011

2. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156-159,1987

3. De Berardis B, Civitelli G, Condello M, Lista P, Pozzi R, Arancia G, Meschini S: Exposure to ZnO nanoparticles induces oxidative stress and cytotoxicity in human colon carcinoma cells. Toxicol Appl Pharmacol 246:116-127, 2010

4. Deng X, Luan Q, Chen W, Wang Y, Wu M, Zhang H, Jiao Z: Nanosized zinc oxide particles induce neural stem cell apoptosis. Nanotechnology 20:111-118, 2009

5. Emery J, McDonnell P, Burke M, Deen K, Lyn S, Silverman C, Dul E, Appelbaum E, Fogh J, Fogh JM, Orfeo T: One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59(1):221–226, 1977

6. Fan Z, Lu JG: Zinc oxide nanostructures: Synthesis and properties. J Nanosci Nanotechnol 5:1561-1573, 2005

7. Franklin NM, Rogers NJ, Apte SC, Batley GE, Gadd GE, Casey PS: Comparative toxicity of nanoparticulate ZnO, bulk ZnO, and ZnCl2 to a freshwater microalga (Pseudokirchneriella subcapitata): The importance of particle solubility. Environ Sci Technol 41: 8484-8490, 2007

8. Huang CC, Aronstam RS, Chen DR, Huang YW: Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles. Toxicol in Vitro 24:45-55, 2010

9. Huang Z, Zheng X, Yan D, Yin G, Liao X, Kang Y, Yao Y, Huang D, Hao B: Toxicological effect of ZnO nanoparticles based on bacteria. Langmuir 24:4140-4144, 2008

10. Karmakar A, Zhang QL, Zhang YB: Neurotoxicity of nanoscale materials. J Food Drug Anal 22: 147–160, 2014

11. Lee SH, Pie JE, Kim YR, Lee HR, Son SW, Kim MK: Effects of zinc oxide nanoparticles on gene expression profile in human keratinocytes. Mol Cell Toxicol 8:113-118, 2012

12. Migliore L, Uboldi C, Di Bucchianico S, Coppedè F: Nanomaterials and neurodegeneration. Environ Mol Mutagen 56: 149–170, 2015

13. Nguyen HX, O’Barr TJ, Anderson AJ: Polymorphonuclear leukocytes promote neurotoxicity through release of matrix metalloproteinases, reactive oxygen species, and TNF-alpha. J Neurochem 102: 900–912, 2007

14. Osmond MJ, McCall MJ: Zinc oxide nanoparticles in modern sunscreens: An analysis of potential exposure and hazard. Nanotoxicology 4:15-41, 2010

15. Ozic C, Arslanyolu M: Characterization of affinity tag features of recombinant Tetrahymena thermophila glutathione-Stransferase zeta for Tetrahymena protein expression vectors. Turk J Biol 36: 513–526, 2012

16. Pitti R, Marsters S, Ruppert S, Donahue C, Moore A, Ashkenzai A: Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J Biol Chem 271:12687–12690, 1996

17. Song J, Song D, Pyrzynska B, Petruk K, Van Meir E, Hao C: TRAIL triggers apoptosis in human malignant glioma cells through extrinsic and intrinsic pathways. Brain Pathol 13:539– 553, 2003

18. Tian L, Lin B, Wu L, Li K, Liu H, Yan J, Liu X, Xi Z: Neurotoxicity induced by zinc oxide nanoparticles: Age-related differences and interaction. Sci Rep 5: 16117, 2015

19. Urrutia PJ, Mena NP, Núñez MT: The interplay between iron accumulation, mitochondrial dysfunction, and inflammation during the execution step of neurodegenerative disorders. Front Pharmacol 5: 38, 2014

20. Win-Shwe TT, Fujimaki H: Nanoparticles and neurotoxicity. Int J Mol Sci 12: 6267–6280, 2011

21. Xia T, Kovochich M, Liong M, Mädler L, Gilbert B, Shi H, Yeh HI, Zink JI, Nel A: Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano 2: 2121-2134, 2008

22. Yang H, Liu C, Yang D, Zhang H, Xi Z: Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by four typical nanomaterials: The role of particle size, shape and composition. J Appl Toxicol 29:69-78, 2009

23. Yildirim CH, Yucetas SC, Kaya M, Ozic C, Balioglu MB, Ustun H, Tasdemiroglu E, Akbasak A: Alpha-lipoic acid inhibits peridural fibrosis following laminectomy through the inactivation of TGF-β1, PDGF, PAI-1 and IL-6 expressions. Turk Neurosurg 25(1):90-99, 2015

24. Zerafa N, Westwood J, Cretney E, Mitchell S, Waring P, Iezzi M, Smyth M: Cutting edge: TRAIL deficiency accelerates hematological malignancies. J Immunol 175:5586–5590, 2005

25. Zhu X, Zhu L, Duan Z, Qi R, Li Y, Lang Y: Comparative toxicity of several metal oxide nanoparticle aqueous suspensions to Zebrafish (Danio rerio) early developmental stage. J Environ Sci Health A Tox Hazard Subst Environ Eng 43(3): 278-284, 2008

Kaynak Göster

Turkish Neurosurgery
  • ISSN: 1019-5149
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1989