Sexual dimorphism in antennal sensilla of Parthenium beetle Zygogramma bicolorata

In the present study, scanning electron microscopy was used to investigate the ultrastructure of sensilla present on the antennaeof male and female Zygogramma bicolorata. The antennae in this insect consist of the scape, pedicel, and nine flagellomeres. Six types ofsensilla were identified: sensilla trichodea type 1 and 2 (ST1, ST2), sensilla cavitae (SCa), sensilla chaetica (SCh), and sensilla basiconicatype 1 and 2 (SB1, SB2). Sensilla were characterized on the basis of their size and density to find out whether sexual dimorphism existsin this species or not. We observed that the size of the antennomeres was comparatively larger in males than females. Moreover, sensillachaetica were found on all antennomers and have the qualification of being the longest type, suggesting that they probably have a role inmechanoreception. There was an abundance of sensilla trichodea in both males and females, indicating its function as a chemoreceptor.Sensilla basiconica are believed to sense sex pheromones and hence their abundance was more in males as compared to females.

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Alm SR, Hall FR (1986). Antennal sensory structures of Conotrachelus nenuphur (Coleoptera: Curculionidae). Annals of Entomological Society of America 79: 324-333.
Bartlet E, Romani R, Williams IH, Isidoro N (1999). Functional anatomy of sensory structures on the antennae of Psylliodes chrysocephala L. (Coleoptera: Chrysomelidae). International Journal of Insect Morphology and Embryology 28: 291-300.
Buck L, Axel RA (1991). Novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell 65: 175- 187.
Carlson JR (2001). Functional expression of a Drosophila odor receptor. Proceedings of the National Academy of Sciences of the USA 98: 8936-8937.
Chess A, Buck L, Dowling MM, Axel R, Ngai J (1992). Molecular biology of smell: expression of the multigene family encoding putative odorant receptors. Cold Spring Harbor Symposia on Quantitative Biology 57: 505-516.
Crook DJ, Fraser I, Francese JA, Mastro VC (2005). Chemical ecology of the emerald ash borer, Agrilusplani pennies Fairmaire (Coleoptera Buprestidae), in relation to tree volatiles. In: Mastro V, Reardon R, Parra G (editors). Proceedings of the Emerald Ash Borer Research and Technology Development Meeting, 26–27 September 2005, Pittsburg, PA, USA.
Morgantown, WV, USA: USDA Forest Service Forest Health Technology Enterprise Team, p. 63.
Crook DJ, Kerr LM, Mastro VC (2008). Distribution and fine structure of antennal sensilla in emerald ash borer (Coleoptera: Buprestidae). Annals of Entomological Society of America 101: 1103-1111.
De Wilde JE (1976). The olfactory component in host-plant selection in the adult Colorado beetle (Leptinotarsa decemlineata Say). Symp Biol Hung 16: 291-300.
De Wilde JE, Hille Ris Lambers Suverkropp K, Van Tol A (1969). Responses to air flow and airborne plant odour in the Colorado beetle. Netherlands Journal of Plant Pathology 75: 53-57.
Dweck HKM (2009). Antennal sensory receptors of Pteromalus puparum female (Hymenoptera: Pteromalidae), a gregarious pupal endoparasitoid of Pieris rapae. Micron 40: 769-774.
Galizia GC, Silke S (2010). Odor coding in insects. In: Menini A (editor). The Neurobiology of Olfaction. Boca Raton, FL, USA: CRC Press/Taylor & Francis, pp. 35-70.
Hatfield LD, Frazier JL, Coons LB (1976). Antennal sensilla of the pecan weevil, Curculio caryae (Horn) (Coleoptera: Curculionidae). International Journal of Insect Morphology and Embryology 5: 279-287.
Hu F, Zhang GN, Wang JJ (2009). Scanning electron microscopy studies of antennal sensilla of bruchid beetles, Callosobruchus chinensis (L.) and Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Micron 40: 320-326.
Isidoro N, Solinas M (1992). Functional morphology of the antennal chemosensilla of Ceutorhynchus assimilis Payk. (Coleoptera: Curculionidae). Entomologica 27: 69-84.
Katsuto F, Yanagawa A, Tuda M, Sakurai G, Kamitani S, Furuya N (2016). Sexual difference in antennal sensilla abundance, density and size in Callosobruchus rhodesianus (Coleoptera: Chrysomelidae: Bruchinae). Applied Entomology and Zoology 51: 641-651.
Lopes O, Barata EN, Mustaparta H, Araujo J (2002). Fine structure of antennal sensilla basiconica and their detection of plant volatiles in the eucalyptus woodborer, Phoracantha semipunctata Fabricius (Coleoptera: Cerambycidae). Arthropod Structure and Development 31: 1-13.
Mamidala P, Wijeratne AJ, Wijeratne S, Poland T, Qazi SS, Doucet D, Cusson M, Beliveau C, Mittapalli O (2013). Identification of odor-processing genes in the emerald ash borer, Agrilusplani pennis. PLoS One 8: e56555.
Merivee E, Rahi M, Bresciani J, Ravn HP, Luik A (1998). Antennal sensilla of the click beetle, Limonius aeruginosus (Olivier) (Coleoptera: Elateridae). International Journal of Insect Morphology and Embryology 27: 311-318.
Mustaparta H (1973). Olfactory sensilla on the antennae of the pine weevil. Zeitsdchriftf Ür Zellforschung und Mikroskopische. Anatomie 144: 559-571.
Patel S (2011). Harmful and beneficial aspects of Parthenium hysterophorus: an update. Biotech 1: 1-9.
Pitts RJ, Zwiebel LJ (2006). Antennal sensilla of two female anopheline sibling species with differing host ranges. Malaria Journal 5: 26.
Saïd I, Tauban D, Renou M, Mori K, Rochat D (2003). Structure and function of the antennal sensilla of the palm weevil Rhynchophorus palmarum (Coleoptera, Curculionidae). Journal of Insect Physiology 49: 857-872.
Schneider D (1964). Insect antennae. Annual Reviews of Entomology 9: 103-122.
Sen A, Mitchell BK (1995). The role of maxillary and labial palpi and leaf surface waxes in the feeding behavior of the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Phytophaga 7: 83-94.
Sen A, Mitchell BK (2001). Olfaction in the Colorado potato beetle: ultrastructure of antennal sensilla in Leptinotarsa sp. Journal of Biosciences 26: 233-246.
Shields VDC (2008). Ultrastructure of insect sensilla. In: Capinera JL (editor). Encyclopedia of Entomology. Dordrecht, the Netherlands: Springer, pp. 4009-4023.
Sun L, Xiao HJ, Gu SH, Zhou JJ, Guo YY, Liu ZW, Zhang YJ (2014). The antenna‐specific odorant‐binding protein AlinOBP13 of the alfalfa plant bug Adelphocoris lineolatus is expressed specifically in basiconic sensilla and has high binding affinity to terpenoids. Insect Molecular Biology 23: 417-434.
Visser JH, Ave DA (1978). General green leaf volatiles in the olfactory orientation of the Colorado beetle, Leptinotarsa decemlineata. Entomologia Experimentalis et Applicata 24: 738-749.
Vosshall LB, Wong AM, Axel R (2000). An olfactory sensory map in the fly brain. Cell 102: 147-159.
Wang XY, Yang ZQ, Gould YN, Zhang GJ, Liu ES (2010). The biology and ecology of the emerald ash borer, Agrilusplani pennis, in China. Journal of Insect Science 10: 128.
Wang Y, Chen Q, Zhao H, Ren B (2016). Identification and comparison of candidate olfactory genes in the olfactory and non-olfactory organs of elm pest Ambrostomaquadri impressum (Coleoptera: Chrysomelidae) based on transcriptome analysis. PLoS One 11: e0147144.
Wigglesworth VB (1972). The Principles of Insect Physiology. London, UK: Chapman and Hall.
Yi Z, Liu D, Cui X, Shang Z (2016). Morphology and ultrastructure of antennal sensilla in male and female Agrilus mali (Coleoptera: Buprestidae). Journal of Insect Sciences 16: 87.
Zacharuk RY (1980). Ultrastructure and function of insect chemosensilla. Annual Review of Entomology 25: 27-47.
Zacharuk RY (1985). Antennae and sensilla. In: Kerkut GA, Gilbert LI (editors). Comprehensive Insect Physiology, Chemistry and Pharmacology, Vol. 6. Oxford, UK: Pergamon Press, pp. 1-69.
Zhang GH, Li BL, Li CR (2016). Morphology and distribution of antennal sensilla of female Phyllotret astriolata (Fabricius) (Coleoptera: Chrysomelidae). Microscopic Research Technique 79: 219-226.
Zhang L, Ren L, Luo Y, Zo S (2013). Scanning electron microscopy analysis of the cephalic sensilla of Chrysolina aeruginosa Fald. (Coleoptera, Chrysomelidae). Microscopic Research Technique 76: 423-431.
Zhang Y, Ren L, Zhang L, Luo Y (2015). Ultrastructure of antennal and posterior abdominal sensilla in Chlorophorus caragana females. Micron 75: 45-57.