Ophryoscolex Stein, 1858 (Protozoa: Ciliophora: Entodiniomorphida) cinsi hakkında morfolojik ve taksonomik araştırmalar

Bu çalışmada izmir civarındaki salhanelerde kesilen toplam 30 evcil sığır (Bos taurus L.)'dan elde edilen işkembe içerikleri Ophryoscolex cinsine dahil siliyatlar açısından incelenmiştir. Araştırma sonucunda cins içerisine dahil edilen siliyatların önceden bazı araştırıcıların yaptıı gibi kaudal ışınlanma özellikleri (1) ve ikincil ışın halkası sayısına dayalı olarak (2) tür düzeyinde ele alınamıyacakları anlaşıldı. Bu durum sığırlarımızda sözkonusu karakterler açısından bazı ara populasyonların saptanmış olması ve istatistiki analiz yöntemleri ile gösterilmiştir. Sürekli varyasyonlar gösteren bu tip karakterler için en uygun sınıflandırmanın "forma" sınıflandırması olduğu ortaya çıkartılmıştır. Bu nedenle cins yeniden gözden geçirilmiş ve priorite ilkesi gözönünde tutularak önceden iki ayrı tür olarak tanımlanmış olan O. purkynjei ile O. caudatus, O. purkynjei altında birleştirilmiştir. Ayrıca 0. purkynjei'ye dahil 3 yeni forma (O. p. f. purkynjei n. f.. O, p. f. bifıdobicinctus n. f. ve O. p. f. bifidoquadricinctus n. f.) tanımlanmış ve yeni bir tayin anahtarı (s. 28) önerilmiştir.

Morphological and taxonomical investigations on the genus of Ophryoscolex Stein, 1858 (Protozoa: Ciliophora: Entodiniomorphida)

Rumen contents obtained from 30 domesticated cattle {Bos taurus L), slaughtered at abattoirs in the vicinity of Izmir, were surveyed for ciliates belonging to the genus of Ophryoscolex. Results of this survey suggested that the ciliates in this two genus should not be classified to the species level on the basis of caudal spination characteristics or the number of the rings of secondary caudal spines, as previously proposed (1, 2). Populations intermediate in these characteristics were detected in Turkish cattle and analysis of the morphometrical data for all criteria did not indicate significant differences for establishing separate species. It appears that these features, which show continuous variation, are more appropriate for classification as formae. Therefore, this genus has been revised. 0. purkynjei and 0. caudatus, previously described as two different species, are now considered as the same species, 0. purkynjei, according to the priority in Systematic Zoology. Three new formae (0. p. f. purkynjei n. f., O. p. f. bifıdobicinctus n. f. and 0. p. f. bifidoquadricinctus n. f.), all belonging to 0. purkynjei are described and a new key proposed for identification (p. 28).

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  • 1. Dogiel, V. A., Monographie der Familie Ophryoscolecidae. Arch. Protistenkd., 59 (1): 1-288, 1927.
  • 2. Kofoid, C. A. and MacLennan, R. F., Ciliates from Bos indicus Linn. III. Epidinium Crawley, Epiplastron gen. nov. and Ophryoscolex Stein. Univ. Calif. (Berkeley) Publ. Zool., 39: 1-33,1933.
  • 3. Lubinsky, G., Ophryoscolecidae (Ciliata: Entodiniomorphida) of Reindeer (Rangifer tarandus L.) from the Canadian Arctic. I. Entodiniidae. Can. J. Zool., 36: 819-835, 1958.
  • 4. Noirot-TimothÈe, C., Etude d’une Familie des CiliÈs: Les Ophryoscolecidae: Structures et Ultrastructures. Ann. Sci. Nat. Zool. Biol., Ser. 12, 2: 527-7›8 (Tháse, Masson et Cie, Paris), 1960.
  • 5. Ogimoto, K. and Imai, S., Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokyo, 231p, 1981.
  • 6. Dehority, B. A., Protozoa of the Digestive Tract of Herbivorous Mammals. Insect Sci. Applic., 7 (3): 279-296, 1986.
  • 7. Williams, A. G. and Coleman, G. S., The Rumen Protozoa. In: The Rumen Microbial Ecosystem, Hobson, P. N. (ed.), Elsevier Science Publishers Ltd., London, pp. 77-128, 1988.
  • 8. Williams, A. G. and Coleman, G. S., The Rumen Protozoa. Brock/Springer Series in Contemporary Bioscience, SpringerVerlag, New York. 442 pp, 1992.
  • 9. Grain, J., Infusoires CiliÈs (Ordre des Entodiniomorphida). TraitÈ de Zoologie, Grasse, P. (Ed.), 2 (2): 327-364, 1994.
  • 10. Stein., F., Ueber mehrere neue im Pansen der Wiederk‰uer lebende Infusionsthiere. Abh. d. Kais. Böhm. Ges. Wiss., ›0: 69-70, 1858.
  • 11. Eberlein, R., Über die im Wiederkáuermagen vorkommenden Ciliaten Infusorien. Zeitsch. Wiss. Zool., 59 : 233-304, ›895.
  • 12. Da Cunha, A. M., Üeber die Ziliaten, Welche in Brazilien im Magen von Rindern und Schafen Verkommen. Mem. Inst. Oswaldo Cruz, 6: 58-67, 1914.
  • 13. Awerinzew, S. und Mutafáwa, R., Material zur Kenntnis der Infusorien aus dem Magen der Wiederk áuer. Arch. f. Protistenkd., 33: 109-118,1914.
  • 14. Dogiel, V. A., Neue parasitische Infusorien aus dem Magen des Renntieres (Rangifer tarandus). Arch. Rus. Protistol., 4 (1-2): 43- 65, 1925.
  • 15. Crawley, H., Evolution in the Ciliate Family Ophryoscolecidae. Proc. Acad. Nat. Sci. Philad., 75: 393-414, 1923.
  • 16. Dogiel, V. A. and Fedorowa, T., On the Variation and Inheritance of Some Morphological Characters in Ophryoscolex purkynjei (Infusoria, Oligotricha). J. Genetics,16: 257-268, 1926.
  • 17. Göçmen, B., Epidinium Crawley, 1923 (Protozoa: Ciliophora: Entodiniomorphida) Cinsi Hakk›nda Morfolojik ve Taksonomik Araflt›rmalar. Do¤a-Tr. J. of Zoology, 23(Ek say› 2): 831 s. 1999.
  • 18. Becker, E. R. and Talbott, M., The Rumen Protozoan Fauna of the Rumen and Reticulum of American Cattle. Iowa State Univ. J. Sci., 1: 345-373, 1927.
  • 19. Bush, M. and Kofoid, C. A., Ciliates from the Sierra Nevada Bighorn Sheep, Ovis canadensis sierrae Grinned. Univ. Calif. (Berkeley) Publ. Zool., 53: 237-262,1948.
  • 20. Mah, R. A., Factors Influencing the in vitro Culture of the Rumen Ciliate Ophryoscolex purkynjei. J. Protozool., 11 (4): 546-552, 1964.
  • 21. Mah, R. A. and Hungate, R. E., Physiological Studies on the Rumen Ciliate, Ophryoscolex purkynjei Stein. J. Protozool., 12 (1): 131- 136, ›965.
  • 22. Coleman, G. S., The Cultivation of Rumen Entodiniomorphid Protozoa, “Isolation of Anaerobes”, Shapton, D. A. and Board, R. G. (eds.), Academic Press, London and New York, pp. 159-176, 1971.
  • 23. Coleman, G. S., Rumen Ciliate Protozoa, Advances in Parasitology, Lumsden, W. H. R., Muller, R. and Baker, J. R. (eds.), Academic Press, London Academic Press, London, 18: 121-173, 1980.
  • 24. Coleman, G. S., Rumen Entodiniomorphid Protozoa, In Vitro Methods for Parasite Cultivation, Taylor, A. E. R., Muller, R. and Baker, J. R. (eds.), Academic Press, London, pp. 26-51, 1987.
  • 25. Göçmen, B., S›¤›r 0flkembesinde Endosimbiyont Yaflayan Isotricha spp. Stein, 1859 (Isotrichidae, Trichostomatida) Üzerine Ifl›k Mikroskobu Düzeyinde Morfolojik ve Sitolojik Gözlemler. Do¤a-Tr. of Zoology, 17 (3): 289-301, 1993.
  • 26. Öktem, N. ve Göçmen, B., Türkiye Evcil S›¤›r (Bos taurus taurus L.) ‹flkembesinden Yeni Bir Siliyat Grubu (Entodiniomorphida: Ophryoscolecidae) ve Yeni Bir Tür, Entodinium basoglui sp. nov. Hakk›nda, Do¤a-Tr. J. of Zoology, 20 (Ek say›): 271-278, 1996.
  • 27. Göçmen, B. and Öktem, N., New Rumen Ciliates from Turkish Domestic Cattle (Bos taurus L.): I - The Presence of Entodinium dalli Dehority, 1974 with a New Forma, E. dalli f. rudidorsospinatum n. f. and Comparisons with Entodinium williamsi n. sp.. Europ. J. Protistol., 32 (4): 513-666, 1996.
  • 28. Göçmen, B. ve Öktem, N., ‹flkembe Siliyat› Entodinium longinucleatum (Ciliophora: Entodiniomorphida: Entodiniidae)’un Evcil S›¤›rlardaki Taksonomik Durumu. Do¤a-Tr. of Zoology, bask›da.
  • 29. Torun, S., Evcil Koyun (Ovis ammon aries)’un ‹flkembe Siliyat Faunas›. Yüksek Lisans Tezi, E. Ü. Fen Bil. Enst., 88s (+ 10 Levha), 1996.
  • 30. Öktem, N., Göçmen, B. ve Torun, S., Türkiye Evcil Koyun (Ovis ammon aries)’lar›n›n ‹flkembe Siliyat (Protozoa: Ciliophora) Faunas› Hakk›nda Bir Ön Çal›flma Raporu: I- Familya Isotrichidae (Trichostomatida) ve Entodiniidae (Entodiniomorphida). Do¤a-Tr. J. of Zoology, 21(4): 475-502, 1997.
  • 31. Coleman, G.S. and Hall, F. J., Electron Microscopy of the Rumen Ciliate Entodinium caudatum, with Special Reference to the Engulfment of Bacteria and Other Particulate Matter. Tissue and Cell, 1 (4): 607-618, 1969.
  • 32. Puytorac, P. de, Grain, J. et Mignot, J. P., Prácis de Protistologie. Sociáte Nouvelle des Editions Boubáá, Paris. 581p, 1987.
  • 33. Kofoid, C. A. and MacLennan, R. F., Ciliates from Bos indicus Linn. I. The Genus Entodinium Stein. Univ. Calif. (Berkeley) Publ. Zool., 33: 471-544, 1930.
  • 34. Mayr, E., Principles of Systematic Zoology. Mac Graw-Hill Inc., New York. 428p, 1969.
  • 35. Ito, A. and Imai, S., Ciliated Protozoa in the Rumen of HolsteinFriesian Cattle (Bos taurus taurus) in Hokkaido, Japan, with the Description of Two New Species. Zool. Sci., 7 (3): 449-458), 1990.
  • 36. Coleman, G. S.and Reynolds, D. J., The Effect of Sterols and Haemin on the Growth of the Rumen Ciliate Ophryoscolex caudatus and Some Other Entodiniomorphid Protozoa. J. Appl. Bacteriol., 52: 129-134, 1982.
  • 37. Fiorentini, A., Intorno ai protisti dello Stomaco dei bovini (Pavia) (Thessis of Ph. D.), ›890- Sur les Protistes de l’estomac des BovidÈs. J. Micrographie, 14: 23-28, 79-83, 178-183, 1889.
  • 38. Schrenk, H.-G. and Bardele, C. F., The Fine Structure of the Paralabial Organelle in the Rumen Ciliate Ophryoscolex purkinjei Stein, 1858. J. Protozool., 34 (1): 97-104, 1987.
  • 39. Hsiung, T.-S., A General Survey of the Protozoan Fauna of the Chinese Cattle. Bull. Fan Mem. Inst. Biol., 3: 87-107, 1932.
  • 40. Imai, S., Katsuno, M. and Ogimoto, K., Distrubution of Rumen Ciliate Protozoa in Cattle, Sheep and Goat and Experimental Transfaunation of Them. Jpn. J. Zootech. Sci., 49 (7): 494-505, 1978.
  • 41. Ito, A., Imai, S. and Ogimoto, K., Rumen Ciliate Composition and Diversity of Japanese Beef Black Cattle in Comparison with Those of Holstein-Friesian Cattle. J. Vet. Med. Sci., 56 (4): 707-714, 1994.
  • 42. Bonhomme-Florentin, A., Blancou, J. et Latteur, B., …tude des Variations Seasonoires de la Microfaune du Rumen de Zebus. Protistologica, 14 (3):283-289, 1978.
  • 43. Das-Gupta, M., Preliminary Observations on the Protozoan Fauna of the Rumen of Indian Goat, Capra hircus Linn. Arch. f. Protistenkd., 85: 153-172, 1935.
  • 44. Buisson, J., Infusoires nouveaux Parasites D’antilopes Africaines. C.R. Soc. Biol., 89: 1217-1219, 1923.
  • 45. Hsiung, T.-S., The Protozoon Fauna of the Rumen of Chinese Sheep. Bull. Fan Mem. Inst. Biol., 2: 29-43, 1931.
  • 46. Dehority, B. A., Rumen Ciliate Fauna of Alaskan Moose (Alces americana), Musk-ox (Ovibos moschatus) and Dall Mauhtain Sheep (Ovis dalli). J. Protozool., 2› (1): 26-32, 1974.
  • 47. Marinho, A.A. de M., Protozo·rios Ciliados No R˙men de Ovinos Em Pastoreio (Ciliate Protozoa in the Rumen of Grazing Sheep). Rev. Port. CiÍne. Veter., 78 (467): 157-165, 1983.
  • 48. Clarke, R. T. J., Ciliates of the Rumen of Domestic Cattle (Bos taurus L.). N. Z. J. Agric. Res., 7: 248-257, 1964.
  • 49. Banerjee, A. K., Studies on Parasitic Ciliates from Indian Ruminants. Proc. Zool. Soc. Bengal., 8 (2): 87-101, 1955.
  • 50. Shimuzu, M., Kinoshita, M., Fujita, K. and Imai, S., Rumen Ciliate Protozoal Fauna and Composition of the Zebu Cattle, Bos indicus and Water Buffalo, Bubalus bubalis, in Philippines. Bull. Nip. Vet. Zootech. Col., 32:83-88, 1983.
  • 51. Imai, S. and Ogimoto, K., Rumen Ciliate Protozoal Fauna and Bacterial Flora of Zebu Cattle (Bos indicus) and the Water Buffalo (Bubalus bubalis) in Thailand. Jpn. J. Zootech. Sci., 55 (8): 576- 583, ›984.
  • 52. Imai, S., Ciliate Protozoa in the Rumen of Kenyan Zebu Cattle, Bos taurus indicus, with the Description of Four New Species. J. Protozool., 35 (1): 130-136, 1988.
  • 53. Dogiel, V. A., The Phylogeny of the Stomach Infusorians of Ruminants in the Light of Palaentological and Parasitological Data. Quart. J. Microsc. Sci., Ser. 3, 88: 337-343, 1947.
  • 54. Lubinsky, G., Studies on the Evolution of the Ophryoscolecidae (Ciliata: Oligoisotricha). III. Phylogeny of the Ophryoscolecidae based on their Comparative Morphology. Can. J. Zool., 35: 141- 159, 1957.
  • 55. Imai, S., Katsuno, M. and Ogimoto, K., Type of the Pattern of the Rumen Ciliate Composition of the Domestic Ruminants and the Predator-Prey Interaction of Ciliates. Jpn. J. Zootech. Sci., 50 (2): 79-87, 1979.
  • 56. Eadie, J. M., The Mid-Winter Rumen Microfauna of the SeaweedEaten Sheep of North Ronaldshay. Proc. R. Soc. Edinburg Sect. B, 66: 276-287, 1956.
  • 57. Eadie, J. M., Interrelationships between Certain Rumen Ciliate Protozoa. J. Gen. Microbiol., 29 : 579-588, 1962.
Turkish Journal of Zoology-Cover
  • ISSN: 1300-0179
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK