Morphological characterization of hemocyte types in some species belonging to Tettigoniidae and Pamphagidae (Insecta: Orthoptera)

  In the present study, the species Eupholidoptera smyrnensis (Brunner von Wattenwyl, 1882) (Orthoptera: Tettigoniidae), Decticus verrucivorus (Linnaeus, 1758) (Orthoptera: Tettigoniidae), and Glyphotmethis spp. (Orthoptera: Pamphagidae) were studied in order to determine hemocyte types. To that end, hemolymph smear preparations were stained using Wright's stain. According to light microscopic examinations, five hemocyte types, prohemocytes, plasmatocytes, granulocytes, spherulocytes, and oenocytoids, were determined in each species. Prohemocytes are basic and the smallest cell type in the hemolymph. Plasmatocytes show polymorphism and they may have pseudopodia. Granulocytes are characterized by granules in their cytoplasm. Spherulocytes typically include spherical vacuoles. Oenocytoids are the largest cells among hemocyte types. These hemocyte types have similar characteristics in all examined species. Hemocyte measurements were also made. According to these measurements, E. smyrnensis hemocytes were smaller than those of Decticus verrucivorus and Glyphotmethis spp. Although included in different families, the two closest species in terms of hemocyte size were D. verrucivorus and Glyphotmethis spp.

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  • Ademolu KA, Idowu AB, Olatunde G (2010). Hemocyte populations in Zonocerus variegatus (L.) (Orthoptera: Pyrgomorphidae) during post-embryonic development. Acta Entomol Sin 53: 470-473.
  • Anderl I, Vesala L, Ihalainen TO, Vanha-aho LM, Ando I, Ramet M, Hultmark D. (2016). Transdifferentiation and proliferation in two distinct hemocyte lineages in Drosophila melanogaster larvae after wasp infection. PLoS Pathog 12: e1005746.
  • Berger J, Slavickova K (2008). Morphological characterization of hemocytes in the adult linden bug, Pyrrhocoris apterus (L.) (Heteroptera). Zool Stud 47: 466-472.
  • Berger J, Walczysko S, Pávková J, Gutzeit HO (2003). Effects of genistein on insect haemocytes. J Appl Biomed 1: 161-168.
  • Brehelin M, Zachary D (1986). Insect hemocytes: a new classification to rule out the controversy. In: Brehelin M, editor. Immunity in Invertebrates. Berlin, Germany: Springer-Verlag, pp. 36-48.
  • Chapman RF (2013). The Insects: Structure and Function. 5th ed. Cambridge, UK: Cambridge University Press.
  • Duressa TF, Vanlaer R, Huybrechts R (2015). Locust cellular defense against infections: sites of pathogen clearance and hemocyte proliferation. Dev Comp Immunol 48: 244-253.
  • Gupta AP (1985). Cellular elements in hemolymph. In: Kerkut GA, Gilbert LI, editors. Comprehensive Insect Physiology, Biochemistry and Pharmacology. Integument Respiration and Circulation, Vol. 3. New York, NY, USA: Pergamon Press, pp. 401-451.
  • Hillyer JF (2015). Integrated immune and cardiovascular function in pancrustacea: lesson from the insects. Integr Comp Biol 55: 843-855.
  • Hillyer JF (2016). Insect immunology and hematopoiesis. Dev Comp Immunol 58: 102-118.
  • Hoffmann JA (2003). Innate immunity of insects. Curr Opin Immunol 7: 4-10.
  • Jalali J, Salehi R (2008). The hemocyte types, differential and total count in Papilio demoleus L. (Lepidoptera: Papilionidae) during post-embryonic development. Mun Ent Zool 3: 199-216.
  • Lavine MD, Strand MR (2002). Insect hemocytes and their role in immunity. Insect Biochem Mol Biol 32: 1295-1309.
  • Lawrence OP (2008). Hemocytes of insects: their morphology and function. In: Capinera JL, editor. Encyclopedia of Entomology. Berlin, Germany: Springer, pp. 1787-1790.
  • League GP, Hillyer JF (2016). Functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts. BMC Biol 14: 78.
  • Levin DM, Breuer LN, Zhuang S, Anderson SA, Nardi JB, Kanost MR (2005). A hemocyte-specific integrin required for hemocytic encapsulation in the tobacco hornworm, Manduca sexta . Insect Biochem Mol Biol 35: 369-380.
  • Liu F, Xu Q, Zhang Q, Lu A, Beerntsen BT, Ling E (2013). Hemocytes and hematopoiesis in the silkworm, Bombyx mori . Invert Surviv J 10: 102-109.
  • Miranpuri GW, Bidochka MJ, Tourians GKG (1991). Morphology and cytochemistry of hemocytes and analysis of hemolymph from Melanoplus sanguinipes (Orthoptera: Acrididae). J Econ Entomol 84: 371-378.
  • Silina KV (2003a). Electron microscope study of haemolymph cells of Tettigonia cantans (Orthoptera, Tettigonidae). Tsitologiia 45: 357-367.
  • Silina KV (2003b). Electron microscope study of haemolymph cells of Decticus verrucivorus (Orthoptera, Tettigoniidae) in larva and imago stages. Tsitologiia 45: 635-649.
  • Silva JEB, Boleli IC, Simoes ZLP (2002). Hemocyte types and total and differential counts in unparasitized Anastrepha obliqua (Diptera, Tephritidae) larvae. Braz J Biol 62: 689-699.
  • Silva TLA, Vasconcellos LRC, Lopes AH, Souto-Padron T (2013). The immune response of hemocytes of the insect Oncopeltus fasciatus against the flagellate Phytomonas serpens . PLoS One 8: e72076
  • Sokolova IuIa, Tokarev IuS, Lozinskaia IaL, Glupov VV (2000). A morphofunctional analysis of the hemocytes in the cricket Gryllus bimaculatus (Orthoptera: Gryllidae) normally and in acute microsporidiosis due to Nosema gryli . Parazitologia 34: 408-419 (in Russian with English abstract).
  • Stanley H, Haas E, Miller J (2012). Eicosanoids: exploiting insect immunity to improve biological control programs. Insects 3: 492-510.
  • Strand MR (2008). The insect cellular immune response. Insect Sci 15: 1-14.
  • Tsakas S, Marmaras VJ (2010). Insect immunity and its signalling: an overview. Invert Surviv J 7: 228-238.
  • Vilmos P, Kurucz E (1998). Insect immunity: evolutionary roots of the mammalian innate immune system. Immunol Lett 62: 59- 66.
  • Wu G, Liu Y, Ding Y, Yunhong Y (2016). Ultrastructural and functional characterization of circulating hemocytes from Galleria mellonella larva: cell types and their role in the innate immunity. Tissue Cell 48: 297-304.
  • Yamashita M, Iwabuchi K (2001). Bombyx mori prohemocyte division and differentiation in individual microcultures. J Insect Physiol 47: 325-331.