Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River, Turkey

Fırat Nehir sisteminde yaşayan Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae)’nin karyotip, C-bantlama ve nükleolus organizatör bölge analizi yapıldı. Metafaz kromozomları böbrek hücrelerinden elde edildi. Diploid kromozom sayısı 19 çift meta-submetasentrik ve 6 çift subtelosentrik olmak üzere 2n = 50 ve kol sayısı 88 bulundu. Eşey kromozomu farklılaşması gözlenmedi. C-bantlama ile altı çift kromozomun perisentromerlerinde heterokromatin bölge tespit edildi. Orta büyüklükte 2 çift submeta-subtelosentrik kromozomun kısa kollarında NOR gözlendi. Bu çalışmanın, yapılacak olan sitogenetik araştırmalara katkı sağlayacağı düşünülmektedir.

Fırat Nehri’ndeki Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae)’nin karyotip, NOR ve C-bantlama analizi

Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae) living in the Euphrates River system were analyzed in terms of their karyotype, C-banding, and nucleolus organizer region properties. Metaphase chromosome spreads were obtained from kidney cells. The diploid chromosome number was found to be 2n = 50, of which 19 pairs were meta-submetacentric and 6 pairs were subtelocentric, and the fundamental number was found to be 88. No sex chromosome differentiation was observed. C-banding revealed heterochromatin in the pericentromeric regions of 6 pairs of chromosomes. NORs were identified on the short arms of 2 pairs of medium-sized submeta-subtelocentric chromosomes. This study may contribute to other cytogenetic research.

___

  • Amemiya, C.T. and Gold, J.R. 1988. Chromosomal NORs as taxonomic and systematic characters in North American cyprinid fi shes. Genetica 76: 81-90.
  • Amemiya, C.T. and Gold, J.R. 1990. Cytogenetic studies in the North American minnows (Cyprinidae). Hereditas 112: 231-247.
  • Bogutskaya, N.G. 1997. Contribution to the knowledge of leuciscine fi shes of Asia Minor. Part 2. An annotated check list of leuciscine fishes (Leuciscinae, Cyprinidae) of Turkey with descriptions of a new species and two new subspecies. Mitt. Hamb. Zool. Mus. Inst. 94: 161-186.
  • Bogutskaya, N.G., Küçük, F. and Atalay, M.A. 2007. A description of three new species of the genus Pseudophoxinus from Turkey (Teleostei: Cyprinidae: Leuciscinae). Zoosyst. Ross. 15(2): 335- 341.
  • Boron, A. 2001. Comparative chromosomal studies on two minnow fish, Phoxinus phoxinus (Linnaeus, 1758) and Eupallasella perenurus (Pallas, 1814); an associated cytogenetic-taxonomic considerations. Genetica 111: 387-395.
  • Collares-Pereira, M.J. 1992. In vivo direct chromosome preparation (protocol for air drying technique). First International Workshop on Fish Cytogenetic Techniques, France: 15-20.
  • Freyhof, J. and Özuluğ, M. 2006. Pseudophoxinus ninae, a new species from Central Anatolia, Turkey (Teleostei: Cyprinidae). Ichtyol. Explor. Freshwaters 17(3): 255-259.
  • Gaffaroğlu, M. 2003. The karyological analysis of some species from Cyprinidae in Karakaya Dam Lake, PhD thesis, İnönü University, Malatya, 71 pp.
  • Gaffaroğlu, M. and Yüksel, E. 2009. Constitutive heterochromatin in Acanthobrama marmid and Cyprinion macrostomus (Osteichtyes, Cyprinidae). Kafkas Univ. Vet. Fak. Derg. 15(2): 169-172.
  • Gaffaroğlu, M., Yüksel, E. and Rab, P. 2006. Note on the karyotype and NOR phenotype of leuciscine fish Acanthobrama marmid (Osteichthyes, Cyprinidae). Biologia 61(2): 207-209.
  • Gold, J.R., Amemiya, C.T. and Ellison, J.R. 1986. Chromosomal heterochromatin differentiation in North American cyprinid fi shes. Cytologia 51: 557-566.
  • Gold, J.R., Li, Y.C., Shipley, N.S. and Powers, P.K. 1990. Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. J. Fish Biol. 37: 563-575.
  • Howell, W.M. and Black, D.A. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014-1015.
  • Hrbek, T., Stölting, K.N., Bardakci, F., Küçük, F., Wildekamp, R.H. and Meyer, A. 2004. Plate tectonics and biogeographical patterns of the Pseudophoxinus (Pisces: Cypriniformes) species complex of central Anatolia, Turkey. Mol. Phylogenet. Evol. 32(1): 297-308.
  • Kılıç-Demirok, N. and Ünlü, E. 2004. Karyotype of the cyprinid fi sh Alburnoides bipunctatus (Cyprinidae) from the Tigris River. Folia Biol. 52(1-2): 57-59.
  • Kirtiklis, L., Boron, A. and Porycka, K. 2005. Chromosome banding patterns of the gudgeon, Gobio gobio (Actinopterygii, Cyprinidae). Acta Ichthyol. Piscat. 35(2): 119-123.
  • Küçük, F. 2007. Pseudophoxinus alii (Teleostei: Cyprinidae), a new fi sh species from the Antalya region, Turkey. Turk. J. Zool. 31: 99-106.
  • Levan, A., Fredga, K. and Sandberg, A.A. 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52: 201-220.
  • Monteiro, R., Carvalho, C. and Collares-Pereira, M.J. 2009. Karyotype and genome size of Iberochondrostoma almacai (Teleostei, Cyprinidae) and comparison with the sister-species I. lusitanicum. Genet. Mol. Biol. 32(2): 268-275.
  • Ozouf-Costaz, C. and Foresti, F. 1992. Fish cytogenetic research: advances, applications and perspectives. Neth. J. Zool. 42(2-3): 277-290.
  • Rab, P. and Collares-Pereira M.J. 1995. Chromosomes of European cyprinid fi shes (Cyprinidae, Cypriniformes): a review. Folia Zool. 44(3): 193-214.
  • Rab, P., Karakousis, Y. and Rabova, M. 1996. Karyotype, NOR phenotype and C-banding study of Barbus cyclolepis from Greece. Folia Zool. 45: 77-83.
  • Rab, P., Rabova, M., Economidis, P.S. and Triantaphyllidis, C. 2000. Banded karyotype of the Greek endemic cyprinid fish, Pachychilon macedonicum. Ichthyol. Res. 47(1): 107-110.
  • Rab, P., Roth, P. and Arefjev, V.A. 1990. Chromosome studies of European leuciscine fishes (Pisces, Cyprinidae) karyotype of Aspius aspius. Caryologia 43(3-4): 249-255.
  • Rabova, M., Rab, P., Ozouf-Costaz, C., Ene, C. and Wanzeböck, J. 2003. Comparative cytogenetics and chromosomal characteristics of ribosomal DNA in the fish genus Vimba (Cyprinidae). Genetica 118: 83-91.
  • Sola, L. and Gornung, E. 2001. Classical and molecular cytogenetics of the zebrafish, Danio rerio (Cyprinidae, Cypriniformes): an overview. Genetica 111: 397-412.
  • Sumner, A.T. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp. Cell. Res. 75: 304-306.
  • Takai, A. and Ojima, Y. 1988. Chromosomal distribution of C-banded heterochromatin in cyprinid fi shes. Proc. Jpn. Acad. 64(3): 49-52.
Turkish Journal of Zoology-Cover
  • ISSN: 1300-0179
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Türkiye, Güneydoğu Anadolu Bölgesi’nin dört farklı lokalitesinden toplanan Unio elongatulus (Bourguignat, 1860) (Bivalvia: Unionidae) midyesinin fosfolipit ve nötral lipit yağ asiti kompozisyonunun karşılaştırılması

İhsan EKİN, Mehmet BAŞHAN, Rıdvan ŞEŞEN

Two new host records for Pomphorhynchus laevis (Müller, 1776) (Acanthocephala) recorded from Antalya, Turkey: Small bleak (Alburnus baliki Bogutskaya, Küçük & Ünlü, 2000) and Antalya barb (Capoeta antalyensis Battalgil, 1944)

Ali AYDOĞDU, Yılmaz EMRE, Nesrin EMRE, Fahrettin KÜÇÜK

Invasion of freshwater bodies in the Marmara region (northwestern Turkey) by nonnative gibel carp, Carassius gibelio (Bloch, 1782)

Özcan GAYGUSUZ, Hamdi AYDIN, Çiğdem Gürsoy GAYGUSUZ, Nildeniz TOP, Ali Serhan TARKAN, Özgür EMİROĞLU

The use of otolith length and weight measurements in age estimations of three Gobiidae species (Deltentosteus quadrimaculatus, Gobius niger, and Lesueurigobius friesii)

Akın Türker İLKYAZ, Hasan Tuncay KINACIGİL, Gülnur METİN

The structural organization and functional aspects of the olfactory epithelium of tigerperch, Terapon jarbua (Forsskål, 1775) (Perciformes: Terapontidae)

Padmanabha CHAKRABARTI, Saroj Kumar GHOSH

A comparison of the fatty acid composition of the phospholipid and neutral lipid of Unio elongatulus (Bourguignat, 1860) (Bivalvia: Unionidae) mussels from 4 different localities in southeastern Anatolia, Turkey

Mehmet BAŞHAN, İhsan EKİN, Rıdvan ŞEŞEN

Marmara Bölgesi (Kuzey-Batı Türkiye) içsularının egzotik gümüşi havuz balığı Carassius gibelio (Bloch, 1782) tarafından istilası

Hamdi AYDIN, Özcan GAYGUSUZ, Ali Serhan TARKAN, Nildeniz TOP, Özgür EMİROĞLU

Roosting of tree sparrow (Passer montanus) and house sparrow (Passer domesticus) in white stork (Ciconia ciconia) nests during winter

Marcin TOBOLKA

Range extension and translocation for Rhodeus amarus (Bloch, 1782) (Actinopterygii: Cyprinidae) in northwestern Iran

Hamid Reza ESMAEILI, Narges NAZARI, Ali GHOLAMIFARD

Seasonal patterns of the zooplankton community in the shallow, brackish Liman Lake in Kızılırmak Delta, Turkey

Yasemin SAYGI, Ertunç GÜNDÜZ, Fatma Yıldız DEMİRKALP, Selim Süalp ÇAĞLAR