Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats

Öz The aim of the present study was to clarify the effects of silymarin on experimental testicular ischemia / reperfusion injury. A total of 40 Wistar albino rats (10–12 weeks of age, weighing 280–300 g) were randomly divided into five groups. Control group: No surgical procedures were performed. Torsion 3 h / detorsion 3 h group; torsion 3 h / detorsion 24 h group; torsion 3 h / detorsion 3 h + silymarin (250 mg/kg) group; and torsion 3 h / detorsion 24 h + silymarin (250 mg/kg) group. In the study, 720° torsion was applied to the left testicle. At the end of the study, blood was collected from the rats, and an orchiectomy was performed on the left testicles. It was found that tumor necrosis factor alpha (Tnf-α) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expression levels in testicular tissue increased significantly in torsion/detorsion groups, and the expression levels decreased significantly with silymarin administration. In addition, in the testicular tissue of the torsion/detorsion groups, glutathione (GSH) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities decreased, while malondialdehyde (MDA) levels increased. It was found that the parameters specified were reversed with the administration of silymarin. Based on our findings, we can say that silymarin reduces testicular injury by activating antioxidant mechanisms in ischemia/reperfusion injury and minimizing the inflammatory response.

Kaynakça

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Kaynak Göster

Bibtex @araştırma makalesi { tbtkzoology972822, journal = {Turkish Journal of Zoology}, issn = {1300-0179}, eissn = {1303-6114}, address = {}, publisher = {TÜBİTAK}, year = {2021}, volume = {45}, pages = {267 - 276}, doi = {}, title = {Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats}, key = {cite}, author = {Belhan, Saadet and Yıldırım, Serkan and Kayıkcı, Caner and Kömüroğlu, Ahmet Ufuk and Özdek, Uğur and Kuşcu, Yağmur} }
APA Belhan, S , Yıldırım, S , Kayıkcı, C , Kömüroğlu, A , Özdek, U , Kuşcu, Y . (2021). Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats . Turkish Journal of Zoology , 45 (4) , 267-276 .
MLA Belhan, S , Yıldırım, S , Kayıkcı, C , Kömüroğlu, A , Özdek, U , Kuşcu, Y . "Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats" . Turkish Journal of Zoology 45 (2021 ): 267-276 <
Chicago Belhan, S , Yıldırım, S , Kayıkcı, C , Kömüroğlu, A , Özdek, U , Kuşcu, Y . "Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats". Turkish Journal of Zoology 45 (2021 ): 267-276
RIS TY - JOUR T1 - Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats AU - Saadet Belhan , Serkan Yıldırım , Caner Kayıkcı , Ahmet Ufuk Kömüroğlu , Uğur Özdek , Yağmur Kuşcu Y1 - 2021 PY - 2021 N1 - DO - T2 - Turkish Journal of Zoology JF - Journal JO - JOR SP - 267 EP - 276 VL - 45 IS - 4 SN - 1300-0179-1303-6114 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Zoology Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats %A Saadet Belhan , Serkan Yıldırım , Caner Kayıkcı , Ahmet Ufuk Kömüroğlu , Uğur Özdek , Yağmur Kuşcu %T Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats %D 2021 %J Turkish Journal of Zoology %P 1300-0179-1303-6114 %V 45 %N 4 %R %U
ISNAD Belhan, Saadet , Yıldırım, Serkan , Kayıkcı, Caner , Kömüroğlu, Ahmet Ufuk , Özdek, Uğur , Kuşcu, Yağmur . "Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats". Turkish Journal of Zoology 45 / 4 (Temmuz 2021): 267-276 .
AMA Belhan S , Yıldırım S , Kayıkcı C , Kömüroğlu A , Özdek U , Kuşcu Y . Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats. Turkish Journal of Zoology. 2021; 45(4): 267-276.
Vancouver Belhan S , Yıldırım S , Kayıkcı C , Kömüroğlu A , Özdek U , Kuşcu Y . Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats. Turkish Journal of Zoology. 2021; 45(4): 267-276.
IEEE S. Belhan , S. Yıldırım , C. Kayıkcı , A. Kömüroğlu , U. Özdek ve Y. Kuşcu , "Investigation of the effect of silymarin on oxidative DNA damageand inflammatory markers in ischemia/reperfusion injuryfollowing experimental testicular torsion/detorsion in rats", Turkish Journal of Zoology, c. 45, sayı. 4, ss. 267-276, Tem. 2021