Histopathological effects of Montivipera xanthina venom on rats

In this study, the histopathological changes caused by Montivipera xanthina venom to rat skin, skeletal muscle, and liver tissue were analysed by light microscope. The venom (approximately 2.85 mg/kg) was injected intramuscularly into the gastrocnemius muscle of the rats. The animals were dissected 1, 3, and 6 h after the injection time, and tissue samples were taken. Sections 5 µm thick were taken from paraffin blocks of tissue samples, and the sections were stained with haematoxylin and eosin in order to facilitate examination by light microscope. Examinations showed no damage to the epidermis layer of skin. However, damage to the dermis layer, including oedema, haemorrhage, local bleeding, rare inflammatory cells, strong cell infiltration with mixed characteristics, inflammation around the veins, and fat necrosis, was detected. The damage observed in muscular tissue was myonecrosis, mixed character cell infiltration, haemorrhage, and the formation of inclusion on myofibrils. The liver tissue revealed sinusoidal congestion and hepatocellular degeneration.

Anahtar Kelimeler:

Key words: Montivipera xanthina, histopathology, venom, skin, skeletal muscle, liver

Histopathological effects of Montivipera xanthina venom on rats

Keywords:

Key words: Montivipera xanthina, histopathology, venom, skin, skeletal muscle, liver,

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Acosta, O., Levia, L.C., Peichoto, M.E., Marunak, S., Teibler, P. and Rey, L. 2003. Hemorrhagic activity of the Duvernoy’s gland secretion of the xenodontine colubrid Philodryas patagoniensis from the north-east region of Argentina. Toxicon. 41: 1007- 1012.
Ali, S.A., Alam, J.M., Abbasi, A., Zaidia, Z.H., Stoeva, S. and Voelter, W. 2000. Sea snake Hydrophis cyanocinctus venom. II. Histopathological changes, induced by a myotoxic phospholipase A2 (PLA2-H1). Toxicon. 38: 687-705.
Arıkan, H., Kumlutaş, Y., Türkozan, O. and Baran, İ. 2003. Electrophoretic patterns of some Viper venoms from Turkey. Turk. J. Zool. 27: 239-242.
Aznaurian, V.A. and Amiryan, S.V. 2006. Histopathological changes induced by the venom of the snake Vipera raddei (Armanian adder). Toxicon. 47: 141-143.
Balija, M.L., Vrdoljak, A., Habjanec, L., Dojnovic, B., Halassy, B., Vranesic, B. and Tomasic, J. 2005. Th e variability of Vipera ammodytes ammodytes venom from Croatia - biochemical properties and biological activity. Comp. Biochem. Physiol. 140: 257-263.
Baran, İ. 2005. Türkiye Amfi bi ve Sürüngenleri. TÜBİTAK Popüler Bilim Kitapları, Ankara.
Baran, İ. and Atatür, M.K. 1998. Türkiye Herpetofaunası. T.C. Çevre Bakanlığı, Ankara.
Başoğlu, M. and Baran, İ. 1980. Türkiye Sürüngenleri Kısım II. Yılanlar. Ege Üniversitesi Fen. Fak. Kitaplar Serisi 81, İzmir.
Bjarnason J.B. and Fox, J.E. 1989. Hemorrhagic toxins from snake venoms. J. Toxicol. Toxin Reviews 7: 121-209.
Chang, P., Hung, D.Y., Siebert, G.A., Bridle, K. and Roberts, M.S. 2005. Th erapeutic eff ects and possible mechanisms of a snake venom preparation in the fi brotic rat liver. Dig. Dis. Sci. 50: 745-752.
Flowers, H.H. 1963. Th e eff ects of X-irradiation on the biological activity of cottonmouth moccasin (Ancistrodon piscivorus) venom. Toxicon. 1: 131-136.
Goucher, C.R. and Flowers, H.H. 1964. Th e chemical modifi cation of necrogenic and proteolytic activities of venom and the use of EDTA to produce Agkistrodon piscivorus, a venom toxoid. Toxicon. 2: 139-144.
Hanafy, M.S., Rahmy, N.A. and Abd El Khalek, M.M. 1999. Th e dielectric properties of neutron irradiated snake venom and its pathological impact. Phys. Med. Biol. 44: 2343-2366.
Iddon, D., Th eakston, R.D.G. and Ownby L. 1987. A study of the pathogenesis of local skin necrosis induced by Naja nigricollis venom using simple histological staining techniques. Toxicon. 25: 665-672.
Jimenez, N., Escalente, T., Gutierrez, J. M. and Rucavado, A. 2008. Skin pathology induced by snake venom metalloproteinase: acute damage revascularization and re-epithelization in a mouse ear model. J. Invest. Dermatol. 118: 1-8.
Leonardi, A., Gubensek, F. and Krizaj, I. 2001. Purifi cation and characterisation of two hemorrhagic metalloproteinases from the venom of the long-nosed viper Vipera ammodytes ammodytes. Toxicon. 40: 55-62.
McManus, J.F.A. and Mowry, R.W. 1964. Staining Methods: Histologic and Histochemical. 1st ed. Hoeber International Reprint, London and Tokyo.
Okur, M.İ., Yıldırım, A.M. and Köse, R. 2001. Türkiye’deki zehirli yılan ısırmaları ve tedavisi. T. Klin. Tıp Bilimleri 21: 528-532.
Rucavado, A., Nunez, J. and Gutierrez, J. M. 1998. Blister formation and skin damage induced by BaP1, a haemorrhagic metalloproteinase from the venom of the snake Bohtorpus asper. Int. J. Exp. Path. 79: 245-254.
Samel, M., Subbi, J. and Siigur, E. 2002. Biochemical characterisation of fi brinogenolytic serine proteinases from Vipera lebetina snake venom. Toxicon. 40: 51-54.
Teibler, P., Acosta de Perez, O., Marunac, S., Ruiz, R., Koscinzuk, P., Sanchez Negrete, M. and Mussart de Coppo, N. 1999. Lesiones localesy sistemicas inducidas por veneno de Bothrops alternatus (vibora de la cruz) de Argentia. Acta. Toxicol. Argent. 7: 7-10.
Tu, A.T., Homma, M. and Hong, B.S. 1969. Hemorrhagic, myonecrotic, thrombotic and proteolytic activities of viper venoms. Toxicon. 6: 175-178.
Warrell, D.A. 1989. Snake venom in science and clinical medicine. 1. Russell’s viper: biology, venom and treatment of bites. Trans. R. Soc. Trop. Med. Hyg. 83: 732-740.