First DNA barcodes and records of Sciaridae (Insecta, Diptera) from Romania

Sciaridae or the dark-winged fungus gnats fauna of southern and eastern Europe is very poorly known. A total of 29 species were identified from Romania, of which 20 species are reported here for the first time from the country. An updated checklist of dark-winged fungus gnats (Sciaridae) of Romania is provided. DNA barcodes of 39 specimens belonging to 21 species were successfully sequenced, of which some were previously unknown or undetected.