Muradiye KARASU, Eşref YÜKSEL, Muhammet GAFFAROĞLU

Fırat Nehri’ndeki Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae)’nin karyotip, NOR ve C-bantlama analizi

Fırat Nehir sisteminde yaşayan Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae)’nin karyotip, C-bantlama ve nükleolus organizatör bölge analizi yapıldı. Metafaz kromozomları böbrek hücrelerinden elde edildi. Diploid kromozom sayısı 19 çift meta-submetasentrik ve 6 çift subtelosentrik olmak üzere 2n = 50 ve kol sayısı 88 bulundu. Eşey kromozomu farklılaşması gözlenmedi. C-bantlama ile altı çift kromozomun perisentromerlerinde heterokromatin bölge tespit edildi. Orta büyüklükte 2 çift submeta-subtelosentrik kromozomunkısa kollarında NOR gözlendi. Bu çalışmanın, yapılacak olan sitogenetik araştırmalara katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler:

Kromozom, heterokromatin, NOR, Pseudophoxinus firati, Leuciscinae

Karyotype, NORs, and C-banding analysis of Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae) in the Euphrates River, Turkey

Pseudophoxinus firati Bogutskaya, Küçük & Atalay, 2007 (Actinopterygii, Cyprinidae) living in the Euphrates River system were analyzed in terms of their karyotype, C-banding, and nucleolus organizer region properties. Metaphase chromosome spreads were obtained from kidney cells. The diploid chromosome number was found to be 2n = 50, of which 19 pairs were meta-submetacentric and 6 pairs were subtelocentric, and the fundamental number was found to be 88. No sex chromosome differentiation was observed. C-banding revealed heterochromatin in the pericentromeric regions of 6 pairs of chromosomes. NORs were identified on the short arms of 2 pairs of medium-sized submeta-subtelocentric chromosomes. This study may contribute to other cytogenetic research.

Keywords:

heterochromatin, NORs, Pseudophoxinus firati, Leuciscinae, Chromosome,

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