Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species

Occurrence and distribution of enzyme-producing bacteria in the proximal (PI), middle (MI), and distal (DI) segments of the gastrointestinal tracts of 4 brackish water teleosts (Scatophagus argus, Terapon jarbua, Mystus gulio, and Etroplus suratensis) have been investigated. Data were presented as log viable counts g-1 intestine (LVC). The heterotrophic bacterial population had the highest occurrence in the DI regions of all fish species studied except M. gulio. Proteolytic and amylolytic bacteria had the highest occurrence in the DI of M. gulio (LVC = 5.50 and 5.93, respectively), while cellulolytic and lipolytic populations exhibited highest occurrences in the DI regions of T. jarbua (LVC = 6.33) and S. argus (LVC = 5.78), respectively. Out of the 81 bacterial isolates, the most promising 3 isolates were determined through quantitative enzyme assay and studied through 16S rRNA gene sequence analysis for identification. Both the strains SA2.2 isolated from S. argus and TJ2.3 isolated from T. jarbua showed high similarity to different strains of Brevibacillus parabrevis, while another strain, MG4.2, isolated from M. gulio, was similar to Bacillus licheniformis. The NCBI GenBank accession numbers of the 16S rRNA gene sequences for isolates SA2.2, TJ2.3, and MG4.2 were KF377322, KF377324, and KF377323, respectively. The present study might offer scope for further research to evaluate prospects for application of the gut-associated extracellular enzyme-producing bacteria in brackish water aquaculture.

Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species

Occurrence and distribution of enzyme-producing bacteria in the proximal (PI), middle (MI), and distal (DI) segments of the gastrointestinal tracts of 4 brackish water teleosts (Scatophagus argus, Terapon jarbua, Mystus gulio, and Etroplus suratensis) have been investigated. Data were presented as log viable counts g-1 intestine (LVC). The heterotrophic bacterial population had the highest occurrence in the DI regions of all fish species studied except M. gulio. Proteolytic and amylolytic bacteria had the highest occurrence in the DI of M. gulio (LVC = 5.50 and 5.93, respectively), while cellulolytic and lipolytic populations exhibited highest occurrences in the DI regions of T. jarbua (LVC = 6.33) and S. argus (LVC = 5.78), respectively. Out of the 81 bacterial isolates, the most promising 3 isolates were determined through quantitative enzyme assay and studied through 16S rRNA gene sequence analysis for identification. Both the strains SA2.2 isolated from S. argus and TJ2.3 isolated from T. jarbua showed high similarity to different strains of Brevibacillus parabrevis, while another strain, MG4.2, isolated from M. gulio, was similar to Bacillus licheniformis. The NCBI GenBank accession numbers of the 16S rRNA gene sequences for isolates SA2.2, TJ2.3, and MG4.2 were KF377322, KF377324, and KF377323, respectively. The present study might offer scope for further research to evaluate prospects for application of the gut-associated extracellular enzyme-producing bacteria in brackish water aquaculture.

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Bibtex @ { tbtkzoology152940, journal = {Turkish Journal of Zoology}, issn = {1300-0179}, eissn = {1303-6114}, address = {}, publisher = {TÜBİTAK}, year = {2014}, volume = {38}, pages = {79 - 88}, doi = {10.3906/sag-1211-39}, title = {Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species}, key = {cite}, author = {Das, Paramita and Mandal, Sudipta and Khan, Argha and Manna, Sanjib Kumar and Ghosh, Koushik} }
APA Das, P , Mandal, S , Khan, A , Manna, S , Ghosh, K . (2014). Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species . Turkish Journal of Zoology , 38 (1) , 79-88 . DOI: 10.3906/sag-1211-39
MLA Das, P , Mandal, S , Khan, A , Manna, S , Ghosh, K . "Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species" . Turkish Journal of Zoology 38 (2014 ): 79-88 <
Chicago Das, P , Mandal, S , Khan, A , Manna, S , Ghosh, K . "Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species". Turkish Journal of Zoology 38 (2014 ): 79-88
RIS TY - JOUR T1 - Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species AU - Paramita Das , Sudipta Mandal , Argha Khan , Sanjib Kumar Manna , Koushik Ghosh Y1 - 2014 PY - 2014 N1 - doi: 10.3906/sag-1211-39 DO - 10.3906/sag-1211-39 T2 - Turkish Journal of Zoology JF - Journal JO - JOR SP - 79 EP - 88 VL - 38 IS - 1 SN - 1300-0179-1303-6114 M3 - doi: 10.3906/sag-1211-39 UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Zoology Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species %A Paramita Das , Sudipta Mandal , Argha Khan , Sanjib Kumar Manna , Koushik Ghosh %T Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species %D 2014 %J Turkish Journal of Zoology %P 1300-0179-1303-6114 %V 38 %N 1 %R doi: 10.3906/sag-1211-39 %U 10.3906/sag-1211-39
ISNAD Das, Paramita , Mandal, Sudipta , Khan, Argha , Manna, Sanjib Kumar , Ghosh, Koushik . "Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species". Turkish Journal of Zoology 38 / 1 (Mayıs 2014): 79-88 .
AMA Das P , Mandal S , Khan A , Manna S , Ghosh K . Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species. Turkish Journal of Zoology. 2014; 38(1): 79-88.
Vancouver Das P , Mandal S , Khan A , Manna S , Ghosh K . Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species. Turkish Journal of Zoology. 2014; 38(1): 79-88.
IEEE P. Das , S. Mandal , A. Khan , S. Manna ve K. Ghosh , "Distribution of extracellular enzyme-producing bacteria in the digestive tracts of 4 brackish water fish species", Turkish Journal of Zoology, c. 38, sayı. 1, ss. 79-88, May. 2014, doi:10.3906/sag-1211-39
Turkish Journal of Zoology
  • ISSN: 1300-0179
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

38.3b20.1b

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