Characterization of interferon alpha of major histocompatibility complex class I in Punjab urial ( Ovis vignei punjabiensis )

Characterization of interferon alpha of major histocompatibility complex class I in Punjab urial ( Ovis vignei punjabiensis )

We have characterized the endangered Punjab urial via its interferon alpha-A gene. DNA extraction was done from blood,followed by PCR and sequencing using a 3130xl genetic analyzer. Single nucleotide polymorphism and phylogenetic analyses wereperformed with BioEdit 7.0.9.0 and MEGA 6.1. The highly polymorphic nature revealed will lead to identifying molecular markers forbreeding purposes.

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