A study of genetic diversity among different population of Orthochirus sp. based on cytochrome C oxidase subunit I and 16srRNA sequencing
Orthochirus sp. (Family: Buthidae) is widespread throughout the world. There are 17 species, 7 of which have been reported in Khuzestan Province of Iran, including the most diverse species related to the scorpion. In the present study, specimens of O. iranus, O. farzanpay, O. zagrosensis, and O. stockwelli were collected from different areas in this province. Phylogenetic analysis was conducted through duplicating and sequencing the cytochrome c oxidase subunit 1 (COXI) and 16SrRNA genes and comparing their genetic diversity. Orthochirus iranus had the highest genetic distance from O. stockwelli, while the lowest genetic distance was observed between O. farzanpay and O. stockwelli. In addition, the highest interspecific divergence was reported between the species of O. iranus and O. zagrosensis, while the lowest interspecific divergence was found between the species of O. farzanpay and O. stockwelli. The results indicated that cytochrome c oxidase (COXI) and 16SrRNA genes have species identification value.
___
- Fet V, Gantenbein B, Gromov AV, Lowe G, Lourenco WR (2003).
The first molecular phylogeny of Buthidae (Scorpiones).
Euscorpius 4: 1-10. DOI: 10.3109/24701394.2016.1149830
- Fet V (1989). A catalogue of scorpions (Chelicerata: Scorpiones) of
the USSR. Review Museum Society National Bergamo 13: 73-
171.
- Fet V, Lowe G (2000). Family Buthidae C.L. Koch, 1837. In: Fet V,
Sissom WD, Lowe G, Braunwalder ME, editors. Catalog of the
Scorpions of the World (1758–1998). New York: New York
Entomological Society, pp. 54-286.
- Gantenbein B, Fet V, Largiadèr CR, Scholl A (1998). First DNA
phylogeny of the genus Euscorpius Thorell, 1876 (Scorpiones:
Euscorpiidae) and its bearing on the taxonomy and
biogeography of this genus. Biogeographica 75: 59-72. DOI:
10.3897/zookeys.219.3597
- Jafari H, Salabi F, Jelodar A, Navidpour SH, Jahanifard E et
al. (2018). Phylogenetic study on Orthochirus iranus by
using morphological and molecular methods (Scorpiones:
Buthidae). Journal of Entomology and Zoology Studies 6(3):
304-309.
- Kovařík F (2004). Revision and taxonomic position of genera
Afghanorthochirus Lourenço & Vachon, Baloorthochirus
Kovařík, Butheolus Simon,Nanobuthus Pocock, Orthochiroides
Kovařík, Pakistanorthochirus Lourenço, and Asian Orthochirus
Karsch, with descriptions of twelve new species (Scorpiones,
Buthidae). Euscorpius 16: 1-33.
- Kovařík F, Fet V (2006). Taxonomic position of the genus Simonoides
Vachon et Farzanpay and description of a new species of
Orthochirus Karsch from Iran (Scorpiones: Buthidae).
Euscorpius 38: 1-10.
- Mirshamsi O, Sari A, Elahi E, Hosseinie S (2010). Phylogenetic
relationships of Mesobuthus eupeus (C.L. Koch, 1839) inferred
from COI sequences (Scorpiones: Buthidae). Journal of Natural
History 44: 2851-2872. DOI: 10.1080/00222933.2010.512400
- Mirshamsi O, Sari A, Hosseinie S (2011). History and current
checklist of Iran’s scorpiofauna (Arachnida: Scorpiones).
Progress in Biological Sciences 1: 16-28.
- Mirshamsi O, Azghadi S, Navidpour S, Aliabadian M, Kovařík F
(2013). Odontobuthus tirgari sp. nov. (Scorpiones, Buthidae)
from the eastern region of the Iranian Plateau. Zootaxa 3731:
153-170 DOI: 10.11646/zootaxa.3731.1.7
- Navidpour SH (2015). An annotated checklist of scorpions in south
and southwestern parts of Iran. International Journal of Fauna
and Biological Studies 2(3): 9-15.
- Navidpour SH, Ezatkhah M, Kovařík F, Soleglad ME, Fet V (2011).
Scorpions of Iran (Arachnida: Scorpiones). Part VII Kerman
Province. Euscorpius 131: 1-32.
- Navidpour SH, Kovařík F, Soleglad ME, Fet V (2008). Scorpions
of Iran (Arachnida, Scorpiones). Part I Khuzestan Province.
Euscorpius 65: 1-41.
- Ozkan O, Ahmet C, Zafer K (2010). A study on the genetic diversity of
Androctonus crassicauda (Olivier, 1807; Scorpiones Buthidae)
from Turkey. The Journal of Venomous Animals and Toxins
including Tropical Diseases 16(4): 599-606. DOI: 10.1590/
S1678-91992010000400010
- Vachon M (1974). Étude des caractères utilisés pour classer
les familles et les genres de Scorpions (Arachnides) La
trichobothriotaxie en arachnologie. Sigles trichobothriaux
et types de trichobothriotaxie chez les scorpions. Bulletin du
Muséum National d’Histoire Naturelle 3: 857-958.