In order to identify the plant parasitic nematodes associated with Berberis vulgaris, 130 soil samples were collected from the rhizosphere of this plant in the South Khorasan Province of Iran during 2012-2014. Among the identified species, four species, Merlinius acuminatus, Geocenamus dobroticus, Paratylenchus vandenbrandei, and Criconemoides morgensis, are new records for the Iranian nematode fauna.