Insect-specific peptides in the venom of wolf spiders (Araneae: Lycosidae)
The venom of two dominant species of wolf spiders, Pardosa sumatrana Thorell and Pardosa birmanica Simon, was extracted and characterized. Insecticidal potential of crude venom and selected peptide fractions (i.e. 35-kDa fraction of both spiders) was evaluated in the laboratory using Rhopalosiphum padi (Linnaeus) (Homoptera: Aphididae) as a model pest. Results of the study showed that both the crude venom and the protein fractions caused significantly higher mortality in treated aphids compared to the control. It is concluded that both the crude venom and the protein fractions possess insecticidal potential.
___
- Castro CS, Silvestre FG, Araujo SC, Gabriel-De, MY, Mangili
OC, Cruz I, Chavez-Olortegui C, Kalapothakis E (2004).
Identification and molecular cloning of insecticidal toxins
from the venom of the brown spider
Loxosceles intermedia
.
Toxicon 44: 273-280.
- Chaim OM, Trevisan-Silva D, Chaves-Moreira D, Wille AC, Ferrer
VP, Matsubara FH, Mangili OC, Da-Silveira RB, Gremski LH,
Gremski W et al. (2011). Brown spider (
Loxosceles
genus)
venom toxins: tools for biological purposes. Toxins (Basel) 3:
309-344.
- Corzo G, Escoubas P (2003). Pharmacologically active spider peptide
toxins. Cell Mol Life Sci 60: 2409-2426.
- Devaraja S, Girish KS, Devaraj VR, Kemparaju K (2010a). Factor
Xa-like and Fibrinogen lytic activities of a serine protease from
Hippasa agelenoides
spider venom gland extract. J Thromb
Thrombolysis 29: 119-126.
- Devaraja S, Girish KS, Gowtham YJ, Kemparaju K (2010b). The
hagprotease-II is a fibrinogenase from
Hippasa agelenoides
spider venom gland extract: purification, characterization and
its role in hemostasis. Toxicon
57: 248-258.
- Frontali N, Ceccarelli B, Gorio A, Mauro A, Siekeitz P, Tzeng M,
Hurlbut WP (1976). Purification from black widow spider
venom of a protein factor causing the depletion of synaptic
vesicles at neuromuscular junctions.
J Cell Biol 68: 462-479.
- Glare T, Caradus J, Gelernter W, Jackson T, Keyhani N, Köhl
J, Marrone P, Morin L, Stewart A (2012). Have bio pesticides
come of age? Trends
Biotechnol 30: 250-258.
- Irshad M (2001). Aphids and their biological control in Pakistan.
Pakistan
J Biol Sci
4: 537-541.
- King GF (2004). The wonderful world of spiders: preface to the
special Toxicon issue on spider venoms. Toxicon 43: 471-476.
- Leng P, Zhang Z, Guangtang P, Zhao M (2011). Applications and
development trends in biopesticides. Afr J Biotechnol
10:
19864-19873.
- Nauen R, Bretschneider T (2002). New modes of action of
insecticides. Pesticide Outlook 13: 241-245.
- Nicholson GM (2007). Insect-selective spider toxins targeting
voltage-gated sodium channels. Toxicon 49: 490-512.
- Parodi J, Navarrete P, Marconi M, Gutiérrez RS, Martínez-Torres A,
Mejías FR (2010). Tetraethyl ammonium-sensitive K
+
current
in the bovine spermatozoa and its blocking by the venom of the
Chilean
Latrodectus mactans
. Syst Biol Reprod Med 56: 37-43.
- Qaim M, Zilberman D (2003). Yield effects of genetically modified
crops in developing countries. Science 299: 900-902.
- Quistad GB, Dennis PA, Skinner WS (1992). Insecticidal activity
of spider (Araneae), centipede (Chilopoda), scorpion
(Scorpionidae), and snake (Serpentes) venoms. J Econ
Entomol 85: 33-39.
- Richardson M, Pimenta AMC, Bemquerer MP, Santoro MM,
Beirao PSL, Lima ME, Figueiredo SG, Bloch JC, Vasconcelos
EAR, Campos FAP et al. (2006). Comparison of the partial
proteomes of the venoms of Brazilian spiders of the genus
Phoneutria
. Comp
Biochem Physiol 142: 173-187.
- Saez NJ, Senff S, Jensen JE, Er SY, Herzig V, Rash LD, King GF (2010).
Spider-venom peptides as therapeutics. Toxins 2: 2851-2871.
- Sambrook JF, Russell DW (2001).
Molecular Cloning: A Laboratory
Manual
. 3rd ed. Cold Spring Harbor, New York: Cold Spring
Harbor Laboratory.
- Schroeder FC, Taggi AE, Gronquist M, Malik RU, Grant JB, Eisner
T, Meinwald J (2008). NMR-spectroscopic screening of spider
venom reveals sulfated nucleosides as major components for
the brown recluse and related species. P Natl Acad Sci USA
105: 14283-14287.
- Tahir HM, Zafar K, Mishal R, Naseem S, Butt A, Yaqoob R, Ahsan
MM, Arshad M (2015). Potential use of venom of
Odontobuthus
odonturus
(Arachnida: Buthidae) as bio-pesticide against
Rhopalosiphum erysimi
(Homoptera: Aphididae). Pakistan J
Zool
47: 37-40.
- Tedford HW, Sollod BL, Maggio F, King GF (2004). Australian
funnel-web spiders: master insecticide chemists. Toxicon 43:
601-618.
- Vetter I, Davis JL, Rash LD, Anangi R, Mobli M, Alewood PF, Lewis
RJ, King GF (2011). Venomics: a new paradigm for natural
products-based drug discovery. J Amino Acids 40: 15-28.
- Zahra K (2015). Bio-pesticidal potential of low molecular weight
peptides of jumping spiders. M.Phil. thesis, University of
Sargodha, Sargodha, Pakistan.