Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand

Streptococcus uberis is a major bovine mastitis pathogen. Several studies have revealed a persistence of specific strains of S. uberis with enhanced virulence or transmissibility. We aimed to investigate the presence of S. uberis virulence-associated genes including plasminogen activator (pauA), glyceraldehyde-3-phosphate dehydrogenase (gapC), oligopeptide permease (oppF), metal transporter uberis A (mtuA), hyaluronic acid capsules (hasA, hasB, hasC), lactoferrin binding protein (lbp), adhesion protein (sua), and CAMP factor (cfu) as well as the genetic profiles using pulsed-field gel electrophoresis (PFGE) among isolates from cases of clinical mastitis, subclinical mastitis, and intramammary infection with low somatic cell count in dairy cows. A total of 642 milk samples from 178 milking cows of 53 farms in Chiang Mai, Thailand, were collected and cultured. Eighty-eight S. uberis isolates were identified. The most common pattern of virulence-associated genes was hasA+hasB+hasC+sua+gapC+lbp+pauA+oppF+mtuA (34.1%, 30/88). PFGE was performed with 71 isolates, which could be classified into 56 pulsotypes. S. uberis isolates tended to be clustered by farms regardless of the severity of infection. Therefore, molecular epidemiological investigation can be useful in developing an effective control strategy for bovine mastitis caused by S. uberis in dairy farms.

___

  • Vakkamäki J, Taponen S, Heikkilä AM, Pyörälä S. Bacteriological etiology and treatment of mastitis in Finnish dairy herds. Acta Vet Scand 2017; 59: 1-9.
  • Verbeke J, Piepers S, Supré K, De Vliegher S. Pathogen specific incidence rate of clinical mastitis in Flemish dairy herds, severity, and association with herd hygiene. J Dairy Sci 2014; 97: 6926-6934.
  • Boonyayatra S, Thaboonpeng J, Kreausukon K, Suriyasathaporn W. Antimicrobial resistance of mastitis-associated bacteria in lactating dairy cows in Chiang Mai province. Chiang Mai Veterinary Journal 2007; 5: 135-145.
  • Notcovich S, deNicolo G, Williamson NB, Grinberg A, Lopez-Villalobos N, Petrovski KR. The ability of four strains of Streptococcus uberis to induce clinical mastitis after intramammary inoculation in lactating cows. New Zeal Vet J 2016; 64: 218-223.
  • Oliver SP, Gillespie BE, Headrick SJ, Lewis MJ, Dowlen HH. Prevalence, risk factors, and strategies for controlling mastitis in heifers during the periparturient period. Int J Appl Res Vet M 2005; 3: 150-162.
  • Lopez-Benavides MG, Williamson JH, Pullinger GD, Lacy- Hulbert SJ, Cursons RT, Leigh JA. Field observations on the variation of Streptococcus uberis populations in a pasture-based dairy farm. J Dairy Sci 2007; 90: 5558-5566.
  • Rato MG, Bexiga R, Nunes SF, Cavaco LM, Vilela CL, Santos Sanches, I. Molecular epidemiology and population structure of bovine Streptococcus uberis . J Dairy Sci 2008; 91: 4542-4551.
  • Phuektes P, Mansell PD, Dyson RS, Hooper ND, Dick JS, Browning GF. Molecular epidemiology of Streptococcus uberis isolates from dairy cows with mastitis. J Clin Microbiol 2001; 39: 1460-1466.
  • Ward PN, Field TR, Ditcham WGF, Maguin E, Leigh JA. Identification and disruption of two discrete loci encoding hyaluronic acid capsule biosynthesis genes hasA , hasB , and hasC in Streptococcus uberis. Infect Immun 2001; 69: 392-399.
  • Rosey EL, Lincoln RA, Ward PN, Yancey Jr RJ, Leigh JA. PauA: a novel plasminogen activator from Streptococcus uberis. FEMS Microbiol Lett 1999; 178: 27-33.
  • Ward PN, Leigh JA. Characterization of PauB, a novel broad- spectrum plasminogen activator from Streptococcus uberis . J Bacteriol 2002; 184: 119-125.
  • Johnsen LB, Poulsen K, Kilian M, Petersen TE. Purification and cloning of a streptokinase from Streptococcus uberis . Infect Immun 1999; 67: 1072-1078.
  • Moshynskyy I, Jiang M, Fontaine MC, Perez-Casal J, Babiuk LA, Potter AA. Characterization of a bovine lactoferrin binding protein of Streptococcus uberis . Microb Pathog 2003; 35: 203-215.
  • Almeida RA, Luther DA, Park HM, Oliver SP. Identification, isolation, and partial characterization of a novel Streptococcus uberis adhesion molecule (SUAM). Vet Microbiol 2006; 115: 183-191.
  • Jiang M, Babiuk LA, Potter AA. Cloning, sequencing and expression of the CAMP factor gene of Streptococcus uberis. Microb Pathog 1996; 20: 297-307.
  • Pancholi V, Fischetti VA. Glyceraldehyde-3-phosphate dehydrogenase on the surface of group A streptococci is also an ADP-ribosylating enzyme. P Natl Acad Sci USA 1993; 90: 8154-8158.
  • Smith AJ, Kitt AJ, Ward PN, Leigh JA. Isolation and characterization of a mutant strain of Streptococcus uberis , which fails to utilize a plasmin derived beta-casein peptide for the acquisition of methionine. J Appl Microbiol 2002; 93: 631- 639.
  • Smith AL, Ward PN, Field TR, Jones CL, Lincoln RA, Leigh JA. MtuA, a lipoprotein receptor antigen from Streptococcus uberis , is responsible for acquisition of manganese during growth in milk and is essential for infection of the lactating bovine mammary gland. Infect Immun 2003; 71: 4842-4849.
  • Oliver SP, Hogan JS, Jayarao BM, Owens WE. Microbiological Procedures for the Diagnosis of Bovine Udder Infection and Determination of Milk Quality. 4th ed. Verona, WI, USA: National Mastitis Council Inc.; 2004.
  • Hassan AA, Khan IU, Abdulmawjood A, Lammler C. Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis. J Clin Microbiol 2001; 39: 1618-1621.
  • Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-2239.
  • Grundmann H, Hori S, Tanner G. Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms. J Clin Microbiol 2001; 39: 4190-4192.
  • R Core Team. R: A Language and Environmental for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing; 2013.
  • Jayarao BM, Gillespie BE, Lewis MJ, Dowlen HH, Oliver SP. Epidemiology of Streptococcus uberis intramammary infections in a dairy herd. Zentralbl Veterinarmed B 1999; 46: 433-442.
  • Abureema S, Smooker P, Malmo J, Deighton M. Molecular epidemiology of recurrent clinical mastitis due to Streptococcus uberis : evidence of both an environmental source and recurring infection with the same strain. J Dairy Sci 2014; 97: 285-290.
  • Tomita T, Meehan B, Wongkattiya N, Malmo J, Pullinger G, Leigh J, Deighton M. Identification of Streptococcus uberis multilocus sequence types highly associated with mastitis. Appl Environ Microbiol 2008; 74: 114-124.
  • Reinoso EB, Lasagno MC, Dieser SA, Odierno LM. Distribution of virulence-associated genes in Streptococcus uberis isolated from bovine mastitis. FEMS Microbiol Lett 2011; 318: 183-188.
  • Coffey TJ, Pullinger GD, Urwin R, Jolley KA, Wilson SM, Maiden MC, Leigh JA. First insights into the evolution of Streptococcus uberis : a multilocus sequence typing scheme that enables investigation of its population biology. Appl Environ Microbiol 2006; 72: 1420-1428.
  • Pullinger GD, Lopez-Benavides M, Coffey TJ, Williamson JH, Cursons RT, Summers E, Lacy-Hulbert J, Maiden MC, Leigh JA. Application of Streptococcus uberis multilocus sequence typing: analysis of the population structure detected among environmental and bovine isolates from New Zealand and the United Kingdom. Appl Environ Microbiol 2006; 72: 1429- 1436.
  • Zadoks RN, Schukken YH, Wiedmann M. Multilocus sequence typing of Streptococcus uberis provides sensitive and epidemiologically relevant subtype information and reveals positive selection in the virulence gene pauA . J Clin Microbiol 2005; 43: 2407-2417.
  • Ward PN, Holden MTG, Leigh JA, Lennard, N, Bignell, A, Barron A, Clark L, Quail MA, Woodward J, Barrell BG et al. Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis . BMC Genomics 2009; 10: 54.
  • Field TR, Ward PN, Pedersen LH, Leigh JA. The hyaluronic acid capsule of Streptococcus uberis is not required for the development of infection and clinical mastitis. Infect Immun 2003; 71: 132-139.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Flavia RUXANDA, Cristian RAŢIU, Bianca MATOSZ, Radu CONSTANTINESCU, Viorel MICLĂUŞ

Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand

Sukolrat BOONYAYATRA, Prasit THARAVICHITKUL, Stephen Paul OLIVER

Evaluation of biological response modification and immunotherapeutic activities of barley-derived arabinoxylans against coccidiosis in commercial broilers

Zafar IQBAL, Muteen HAMAYUN, Muhammad Irfan ANWA, Masood AKHTAR, Mian Muhammad AWAIS

Clara AKPAN, Nwakaego NWEZE, Cornelius CHUKWU

Hematological and clinical studies in West African Dwarf sheep experimentally infected with Trypanosoma brucei and treated with diminazene aceturate, levamisole, and vitamin A

Clara AKPAN, Nwakaego NWEZE, Cornelius CHUKWU

İgor STOJANOV, Aleksandar MILOVANOVIĆ, Dragana RUŽIĆ-MUSLIĆ, Radomir RATAJAC, Milica ŽIVKOV BALOŠ, Nevena MAKSIMOVIĆ, Jelena APIĆ

Comparative clinical and parasitological efficacy of moxidectin pour-on, ivermectin, and piperazine citrate on Tox o c a r a vitulorum infection in buffalo calves ( Bubalus bubalis ): a randomized clinical trial

Mohamed Abdo RIZK, Salama A. OSMAN, Magdy H. AL-GAABARY, Sabry A. EL-KHODERY

Bilal DİK, Uğur USLU

Sukolrat BOONYAYATRA, Prasit THARAVICHITKUL, Stephen Paul OLIVER

Identification of Sarcocystis spp. by polymerase chain reaction and microscopic examination in various beef products (minced meat, meatballs, fermented sausage)

Atila AKÇA, Mükremin Özkan ARSLAN, Ufuk KAMBER, Gencay Taşkın TAŞÇI, Güven GÜLBAZ