Van yöresinde, sa¤l›kl› görülen süt s›¤›rc›l›¤› iflletmelerindeki hayvanlar›n d›flk›lar›nda rotavirus, Escherichia coli K99 ve O157:H7’ nin varl›¤› üzerine bir araflt›rma yap›ld›. Araflt›rman›n yap›ld›¤› baz› iflletmelerdeki yenido¤an buza¤›larda önceki y›llarda zaman zaman ishal olgular›n›n görüldü¤ü bildirildi. Örneklemenin yap›ld›¤› s›rada da baz› buza¤›larda ishal olgular› tespit edildi. Toplam 312 d›flk› örne¤inde rotaviruslar›n varl›¤›n› belirlemek için PAGE tekni¤i, Escherichia coli K99 ve O157:H7’leri tespit etmek amac›yla da Latex aglutination testi uyguland›. E. coli K99 ve O157:H7 izolatlar›n›n enteropatojenik özelli¤i tavflan lup testi ile ortaya kondu. Sa¤l›kl› görülen hayvanlar›n d›flk›lar›nda rotavirus saptanmazken, 9 ishalli buza¤›n›n yaln›zca 1 tanesinin d›flk›s›nda rotavirus tespit edildi. Toplam 312 d›flk› örne¤inden 235 E. coli izole edildi. ‹zole edilen 235 E. coli suflundan 28’inin K99, 4’ünün de O157:H7 oldu¤u saptand›. Tavflan ince barsak lup testi ile, E. coli K99 sufllar›ndan 14’ünün ve O157:H7 sufllar›n›n da 2’sinin enteropatojenik özellikte oldu¤u belirlendi. Ayr›ca, izole ve identifiye edilen tüm E. coli K99 ve O157:H7 sufllar›n›n yap›lan antibiyogram testinde en çok enrofloxacin ve danofloxacin’e duyarl› olduklar› tespit edildi.

A survey was conducted to determine the prevalence of rotavirus, Escherichia coli K99 and O157:H7 in healthy dairy cattle herds in Van and environs, Turkey. Some of the cattle herds had a history of recurrent neonatal diarrhoea for previous years, and even while sampling was being done, some newborn calves were diarrhoeic. Three hundred and twelve faecal specimens from dairy cattle farms free of clinically apparent diarrhoea were tested both for the presence of rotaviruses by PAGE silver staining and for Escherichia coli K99 and O157:H7 by latex agglutination using specific antisera after the growth of each colony. The enteropathogenic characteristic of K99 and O157:H7 isolates was determined by rabbit ileal loop test. No rotavirus was detected from clinically normal cows, heifers or calves. Only 1 of 9 diarrhoeic calves was found to be positive for rotavirus. Out of the 235 E. coli isolates from non-diarrhoeic animals, 28 were positive for K99 and 4 for O157:H7. While 14 of 28 K99 isolates were found to be positive for enteropathogenic Escherichia coli (EPEC), 2 of 4 O157:H7 isolates were also positive for EPEC. An in vitro antibiotic sensitivity test of K99 and O157:H7 isolates indicated that the most sensitive antibiotics were enrofloxacin and danofloxacin.