Use of Salicin-Rhamnose-Cellobiose-4-Methylumbelliferyl-b-D Glucuronide–Sorbitol MacConkey (SRC-MUG-SMAC) Differential Agar for the Presumptive Identification of Escherichia coli O157 from the Primary Isolation Step

A differential agar for the further detection of presumptive Escherichia coli O157 strains among typical sorbitol negative and cefixime-tellurite resistant strains was investigated. For this purpose, in parallel to colonies of 8 reference strains, 1130 suspect colonies picked from the surface of cefixime-tellurite (CT)-sorbitol MacConkey (SMAC) (CT-SMAC) agar plates of food samples (white cheese, raw milk and ground beef) were transferred to differential agar plates. After a 6-h incubation period at 42 ºC, only 47 (4.15%) of the 1130 colonies appeared as typical E. coli O157 on the differential agar plates (100 x 15 mm) gridded into 25 numbered sections. Salicin (S), rhamnose (R) and cellobiose (C) were tested separately and together in combination with MUG. The best results were obtained from the combined use of the 3 in SMAC medium (SRC-MUG-SMAC). When violet red bile lactose agar (VL) was used in the same manner as SRC-MUG-SMAC, only 16 (1.41%) of the 1130 colonies remained typical. In this procedure, no biochemical test was required for further identification. The use of an SRC-MUG-SMAC plate immediately after the colony selection step appeared to be more discriminative than the addition of these sugars and MUG to the primary isolation media. As a result, the testing of presumptive E. coli O157 colonies using a differential agar plate including MUG and 3 (or more) sugars that are not fermented by this organism is a rapid, easy, precise and relatively inexpensive isolation procedure, and it is particularly ideal for use in routine screening laboratories.

Use of Salicin-Rhamnose-Cellobiose-4-Methylumbelliferyl-b-D Glucuronide–Sorbitol MacConkey (SRC-MUG-SMAC) Differential Agar for the Presumptive Identification of Escherichia coli O157 from the Primary Isolation Step

A differential agar for the further detection of presumptive Escherichia coli O157 strains among typical sorbitol negative and cefixime-tellurite resistant strains was investigated. For this purpose, in parallel to colonies of 8 reference strains, 1130 suspect colonies picked from the surface of cefixime-tellurite (CT)-sorbitol MacConkey (SMAC) (CT-SMAC) agar plates of food samples (white cheese, raw milk and ground beef) were transferred to differential agar plates. After a 6-h incubation period at 42 ºC, only 47 (4.15%) of the 1130 colonies appeared as typical E. coli O157 on the differential agar plates (100 x 15 mm) gridded into 25 numbered sections. Salicin (S), rhamnose (R) and cellobiose (C) were tested separately and together in combination with MUG. The best results were obtained from the combined use of the 3 in SMAC medium (SRC-MUG-SMAC). When violet red bile lactose agar (VL) was used in the same manner as SRC-MUG-SMAC, only 16 (1.41%) of the 1130 colonies remained typical. In this procedure, no biochemical test was required for further identification. The use of an SRC-MUG-SMAC plate immediately after the colony selection step appeared to be more discriminative than the addition of these sugars and MUG to the primary isolation media. As a result, the testing of presumptive E. coli O157 colonies using a differential agar plate including MUG and 3 (or more) sugars that are not fermented by this organism is a rapid, easy, precise and relatively inexpensive isolation procedure, and it is particularly ideal for use in routine screening laboratories.

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Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Immunohistochemical lacolization of FSH cells in the pars distalis of the pituitary gland in five-month-old laying hans

Hatice ERDOST

Türkiye’de Yabani Keklikler Üzerinde ‹lk Defa Bulunan Pseudolichus solutocurtus Dubinin, 1956 (Acarina, Pterolichoidea) ve Harpyrhynchus sp. (Acarina, Harpyrhynchidae Dubinin, 1957) Türleri

Nursel AKSIN, Zerrin ERDOĞMUŞ

Konakçı Faktörlerine Bağlı Olarak Dactylogyrus cornu Linstow, 1878 (Monogenea)’nın Türkiye’nin Sinop Bölgesinden Yakalanan Eğrez Balığı’ndaki (Vimba vimba tenella (Nordmann, 1840)) Enfestasyonu

Ahmet ÖZER, Türkay ÖZTÜRK

Use of salicin-rhamnose-cellobiose-4-methylumbelliferyl-Beta-D Glucuronide-sorbitol MacConkey (SRC-MUG-SMAC) differential agar for the presumptive identification of Escherichia coli 0157 from the primary isolation step

Leyla VATANSEVER, Murat GÜLMEZ, Çiğdem SEZER, Abamüslüm GÜVEN, Berna DUMAN

The Prevalence of Cryptosporidium parvum in Lambs around Konya

Ferda SEVİNÇ, Uğur USLU, Özlem DERİNBAY

Dactylogyrus cornu Linstow, 1878 (Monogenea) infestations on vimba (Vimba vimba tenella (Nordmann, 1840)) cought in the Sinop region of Turkey in relation to the host factors

Türkay ÖZTÜRK, Ahmet ÖZER

Immunohistochemical Localization of FSH Cells in the Pars Distalis of the Pituitary Gland in Five-Month-Old Laying Hens

Hatice ERDOST

Pseudolichus solutocurthus Dubinin, 1956 (Acarina, pterolichoidea) and Harpyrhynchus sp. (Acarina, Harpyrhynchidae Dubinin, 1957) species recorded for the first time on wild partridges in Turkey

Nursel AKSIN, Zerrin ERDOĞMUŞ

Influence of different ligth sources, illumination intensities and storage times on the Vitamin C content in pasteurized milk

Tamer TURGUT, Songül ÇAKMAKÇI

Some Productive Characteristics of F1, B1 and F1XB1 Crossbreeds from Simmental x SAR Crossbreeding

Mehmet N. ORMAN