The protective efficacy of immunoglobulin Y from immunized chickens against Salmonella infections in mice
The aim of this study was to determine the efficacy of immunoglobulin Y (IgY) obtained from chickens immunized with Salmonella vaccines. Chickens were vaccinated three times with inactivated monovalent, bivalent, and combined vaccines. Immunized hen eggs were collected after the third vaccination and IgYs were purified. In total, 100 mice were orally challenged with Salmonella serotypes. After the challenge, IgYs were orally administered to mice. Mice were observed for morbidity and mortality. Fecal samples from the mice were also cultured for the reisolation of Salmonella serotypes. The antibody titers in the serum samples of vaccinated chickens were higher than those of controls (P < 0.001). Neither morbidity nor mortality were observed in these mice. In all of the groups the reisolation numbers of the Salmonella serotypes from internal organs and fecal samples were low (P < 0.001) In conclusion, it is suggested that IgYs from immunized chickens could be used to establish protection against Salmonella infections.
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- Dahlen C, DiLorenzo N, DiCostanzo A. Efficacy of a polyclonal
antibody preparation against respiratory disease pathogens on
cattle morbidity and performance during the step-up period. J
Anim Sci 2008; 86: 195-198.
- Besser TE, Gay CC, McGuire TC, Evermann JF. Passive
immunity to bovine rotavirus infection associated with transfer
of serum antibody into the intestinal lumen. J Virol 1998; 62:
2238-2242.
- Carlander D, Kollberg H, Wejaker PE, Larsson A. Peroral
immunotherapy with yolk antibodies for the prevention and
treatment of enteric infections. Immunol Res 2000; 21: 1-6.
- Kovacs-Nolan J, Mine Y. Egg yolk antibodies for passive
immunity. Annu Rev Food Sci Technol 2012; 3: 163-182.
- Chalghoumi R, Beckers Y, Portetelle D, Thewis A. Hen egg yolk
antibodies (IgY), production and use for passive immunization
against bacterial enteric infections in chicken: a review.
Biotechnol Agron Soc Environ 2009; 13: 295-308.
- Hatta H, Kim M, Yamamoto T. A novel isolation method for
hen egg yolk antibody “IgY”. Agric Biol Chem 1990; 54: 2531-
2535.
- Hirai K, Arimitsu H, Umeda K, Yokota K, Shen L, Ayada
K, Kodama Y, Tsuji T, Hirai Y, Oguma K. Passive oral
immunization by egg yolk immunoglobulin (IgY) to
Vibrio
cholerae
effectively prevents cholera. Acta Med Okay 2010; 64:
169-170.
- Meenatchisundaram S, Parameswari G, Michael A,
Ramalingam S. Studies on pharmacological effects of Russell’s
viper and saw-scaled viper venom and its neutralization by
chicken egg yolk antibodies. Int Immunph 2008; 8: 1067-1073.
- Ikemori Y, Kuroki, M, Peralta RC, Yokoyama H, Kodama Y.
Protection of neonatal calves against fatal enteric colibacillosis
by administration of egg yolk powder from hens immunized
with K99-piliated enterotoxigenic
Escherichia coli
. Am J Vet
Res 1992; 53: 2005-2008.
- Peralta RC, Yokoyama H, Ikemori Y, Kuroki M, Kodama Y.
Passive immunization against experimental salmonellosis in
mice by orally administered hen egg-yolk antibodies specific
for 14-kDa fimbriae of
Salmonella
Enteritidis. J Med Microbiol
1994; 41: 29-35.
- Yokoyama H, Umeda K, Peralta RC, Hashi T, Icatlo FC Jr,
Kuroki M, Ikemori Y, Kodama Y. Oral passive immunization
against experimental salmonellosis in mice using chicken
egg yolk antibodies specific for
Salmonella
Enteritidis and
S.
Typhimurium. Vaccine 1998; 16: 388-393.
- Bizhanov G, Vyshiniauskis G. A comparison of three methods
for extracting IgY from the egg yolk of hens immunized with
Sendai virus. Vet Res Comm 2000; 24: 103-113.
- Lee EN, Sunwoo HH, Menninen K, Sim JS. In vitro studies of
chicken egg yolk antibody (IgY) against
Salmonella
Enteritidis
and
Salmonella
Typhimurium. Poultry Sci 2002; 81: 632-641.
- Dilorenzo N, Dahlen C, Dicostanzo A. Effects of feeding
a polyclonal antibody preparation against
Escherichia coli
O157:H7 on performance, carcass characteristics and
E. coli
O157:H7 fecal shedding of feedlot steers. J Anim Sci 2008; 86:
3023-3032.
- Zhen YH, Jin LJ, Guo J, Li XY, Li Z, Fang R, Xu YP.
Characterization of specific egg yolk immunoglobulin
(IgY) against mastitis-causing
Staphylococcus aureus
. J Appl
Microbiol 2008; 105: 1529-1535.
- Zhen YH, Jin LJ, Guo J, Li XY, Lu YN, Chen J, Xu YP.
Characterization of specific egg yolk immunoglobulin (IgY)
against mastitis-causing
Escherichia coli
. Vet Microbiol 2008;
130: 126-133.
- Zhen YH, Jin LJ, Li XY, Guo J, Li Z, Zhang BJ, Fang R, Xu YP.
Efficacy of specific egg yolk immunoglobulin (IgY) to bovine
mastitis caused by
Staphylococcus aureus
. Vet Microbiol 2009;
133: 317-322.
- Meenatchisundaram S, Michael A, Subbraj T, Diraviam
T, Shanmugam V. Isolation, purification and neutralizing
potential of chicken egg yolk immunoglobulin (IgY) against
mastitis causing
Escherichia coli
in dairy cows in Coimbatore
District. Int J Drug Dev Res 2011; 3: 147-153.
- Hadimli HH, Sakmanoğlu A, Gürbüz E. Determination of
efficacies of
Salmonella
vaccines against
Salmonella
infections
in mice. In: Proceedings of the 11th National Congress of
Veterinary Microbiology (with International Guest Speakers),
Antalya, Turkey; 2014. pp. 22-23 (in Turkish with English
abstract).
- Hadimli HH, Erganiş O, Sayın Z, Yıldırım B. The efficacy of
inactivated
Salmonella
Typhimurium vaccines with ginseng in
mice and sheep. Vet Bil Derg
2007; 23: 17-24 (in Turkish with
English abstract).
- De Meulenaer B, Huyghebaert A. Isolation and purification of
chicken egg yolk immunoglobulins: a review. Food and Agr
Immun 2001; 13: 275-288.
- Chalghoumi R, Thewis A, Portetelle D, Beckers Y. Production
of hen egg yolk immunoglobulins simultaneously directed
against
Salmonella
Enteritidis and
Salmonella
Typhimurium in
the same egg yolk. Poultry Sci 2008; 87: 32-40.
- Hadimli HH, Pınarkara Y, Al-Shattrawi HJ. Determination of
pathogenicities of
Salmonella
isolates in mice. In: Proceedings
of the 11th National Congress of Veterinary Microbiology
(with International Guest Speakers), Antalya, Turkey; 2014.
pp. 20-21 (in Turkish with English abstract).
- Hadimli HH, Pınarkara Y, Sakmanoğlu A, Sayın Z, Erganiş O,
Uslu A, Al-Shattrawi HJ. Serotypes of
Salmonella
isolated from
feces of cattle, buffalo, and camel and sensitivities to antibiotics
in Turkey. Turk J Vet Anim Sci 2017; 41: 193-198.
- Guimarase MCC, Amaral LG, Rangel LBA, Silva IV, Matta
CGF, Matta MFR. Growth inhibition of
Staphylococcus aureus
by chicken egg yolk antibodies. Arch Immunol Ther Exp 2009;
57: 377-382.
- Trott DL, Yang M, Gonzalez J, Larson AE, Tepp WH,
Johnson EA, Cook ME. Egg yolk antibodies for detection and
neutralization of
Clostridium botulinum
type A neurotoxin. J
Food Prot 2009; 72: 1005-1011.
- Vega C, Bok M, Chacana P, Saif L, Fernandez F, Parreno V.
Egg yolk IgY: protection against rotavirus induced diarrhea
and modulatory effect on the systemic and mucosal antibody
responses in newborn calves. Vet Immun Immunpathol 2011;
142: 156-169.
- Wang LH, Hou XJ, Cai K, Li T, Liu YN, Tu W, Xiao L, Bao
SZ, Shi J, Gao X et al. Passive protection of purified yolk
immunoglobulin administered against Shiga toxin 1 in mouse
models. Can J Microbiol 2010; 56: 1003-1010.
- Wang LH, Li XY, Jin JS, Zhou Y, Li SY, Xu YP. Characterization
of chicken egg yolk immunoglobulins (IgYs) specific for
the most prevalent capsular serotypes of mastitis-causing
Staphylococcus aureus
. Vet Mic 2011; 149: 415-421.
- Rofaiil SK, Germin SS. Effectiveness of protective potential of
chicken egg yolk in controlling salmonellosis using laboratory
mice as a model. Egypt J Agric Res 2013; 91: 323-334.