Malgorzata WITESKA, Elzbieta KONDERA, Katarzyna LUGOWSKA

The effects of ichthyophthiriasis on some haematological parameters in common carp

The present study examined the haematological effects of ichthyophthiriasis in common carp. Blood was collected from 4 groups of juvenile carp: 1. healthy; 2. with minor symptoms of ichthyophthiriasis; 3. with severe symptoms of ichthyophthiriasis (moribund); survivors of ichthyophthiriasis after a 3-week recovery period. Blood was subjected to a standard haematological procedure and the following parameters were evaluated: haematocrit (Ht), haemoglobin concentration (Hb), erythrocyte (RBC) and leukocyte (WBC) counts, mean cell volume (MCV), mean cell haemoglobin (MCH), and mean cell haemoglobin concentration (MCHC). The differential leukocyte count and thrombocyte count were calculated using stained blood smears. No change in Ht or Hb, but a significant decrease in RBC compensated by an increase in MCV and MCH were observed. Fish with minor symptoms of disease had a significant increase in WBC (167.4 × 103/mL) without an alteration in the differential count, as compared to the controls (93.3 × 103/mL), while those with severe symptoms had a significant decrease in WBC (48.4 × 103/mL) accompanied by lymphopenia, and an increase in neutrophil and monocyte contribution. After 3 weeks of recovery WBC returned to a level similar to that in the controls (89.5 × 103/mL). In all the parasite-infested fish metabolic activity of phagocytes was significantly reduced, as compared to the controls. In the fish with severe symptoms of disease thrombocytosis and hypercoagulability were observed.

Anahtar Kelimeler:

Key words: Blood, Cyprinus carpio, fish, Ichthyophthirius multifiliis

The effects of ichthyophthiriasis on some haematological parameters in common carp

Keywords:

Key words: Blood, Cyprinus carpio, fish, Ichthyophthirius multifiliis,

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Scholz, T.: Parasites in cultured and feral fish. Vet. Parasitol., 1999; 84: 317-335. 2. Mifsud, C., Rowland, S.J.: Use of salt to control ichthyophthiriosis and prevent saprolegniosis in silver perch, Bidyanus bidyanus. Aquacult. Res., 2008; 39: 1175-1180. 3.
Hines, R.S., Spira, D.T.: Ichthyophthiriasis in the mirror carp
Cyprinus carpio (L.). III. Pathology. J. Fish Biol., 1974; 6: 189- 196. 4.
Tumbol, R.A., Powell, M.D., Nowak, B.F.: Ionic effects of
infection of Ichthyophthirius multifiliis in goldfish. J. Aquat.
Kırkağaç Uzbilek, M., Yavuzcan Yıldız, H.: A report on spontaneous diseases in the culture of grass carp (Ctenopharyngodon idella Val. 1844), Turkey. Turk. J. Vet. Anim. Sci., 2002; 26: 407-410.
Liu, Y.J., Lu C.P.: Role of Ichthyophthirius multifiliis in the infection of Aeromonas hydrophila. J. Vet. Med. B Inf. Dis. Vet. Public Health, 2004; 51: 222-224.
Dickerson, H.W., Clark, T.G.: Immune response of fishes to ciliates. Annu. Rev. Fish Dis., 1996; 6: 107-120.
Sin, Y.M., Ling, K.H., Lam, T.J.: Cell-mediated immune response of goldfish, Carassius auratus (L.), to Ichthyophthirius multifiliis. J. Fish Dis., 1996; 19: 1-7.
Zhao, X., Findly, R.C., Dickerson, H.W.: Cutaneous antibody- secreting cells and B cells in a teleost fish. Dev. Comp. Immunol., 2008; 32: 500-508.
Cross, M.L. Matthews, R.A.: Localized leukocyte response to Ichthyophthirius multifiliis establishment in immune carp Cyprinus carpio L. Vet. Immunol. Immunopathol., 1993; 38: 341-358.
Hines, R.S., Spira, D.T.: Ichthyophthiriasis in the mirror carp. II. Leukocyte response. J. Fish Biol., 1973; 5: 527-534.
Tavares-Dias, M., de Morales, F.R., Martins, M.L., Santana, A.E.: Haematological changes in Oreochromis niloticus (Osteichthyes: Cichlidae) with gill ichthyophthiriasis and saprolegniosis. Bol. Inst. Pesca, Sao Paulo, 2002; 28: 1-9.
Sopińska, A.: Usefulness of the NBT test for evaluation of metabolic activity of peripheral blood granulocytes in common carp. Med. Wet., 1985; 41: 738-740. (article in Polish)
Nikinmaa, M., Huestis, W.H.: Adrenergic swelling of nucleated erythrocytes: cellular mechanisms in a bird, domestic goose, and two teleosts, striped bass and rainbow trout. J. Exp. Biol., 1984; 113: 215-224.
Nikinmaa, M.: Haemoglobin function in vertebrates: evolutionary changes in cellular regulation in hypoxia. Resp. Physiol., 2001; 128: 317-329.
Kind, P.K., Grigg, G.C., Booth, D.T.: Physiological responses to prolonged aquatic hypoxia in the Queensland lungfish Neoceradotus forsteri. Resp. Physiol. Neurobi., 2002; 132: 179- 190.
Speckner, W., Schindler, J.F., Albers, C.: Age-dependent changes in volume and haemoglobin content of erythrocytes in the carp (Cyprinus carpio L.) J. Exp. Biol., 1989; 141: 133-149.
Kurovskaya, L.N., Osadchaya, S.A.: The influence of Ichthyophthirius multifiliis on underyearling carp, Cyprinus carpio. J. Ichthyol., 1993; 33: 81-92.
Alvarez-Pellitero, P.: Fish immunity and parasite infections: from innate immunity to immunoprophylactic prospects. Vet. Immunol. Immunopathol., 2008; 126: 171-198.
Casillas, E., Smith, L.S.: Effect of stress on blood coagulation and haematology in rainbow trout (Salmo gairdneri). J. Fish Biol., 1977; 10: 481-491.
Köllner, B., Fischer, U., Rombout, J.H.W.M., Taverne-Thielec, J.J., Hansen, J.D.: Potential involvement of rainbow trout thrombocytes in immune functions: a study using a panel of monoclonal antibodies and RT-PCR. Dev. Comp. Immunol., 2004; 28: 1049–1062.
Stosik, M., Deptuła, W., Travnicek, M., Baldy-Chudzik, K.: Phagocytic and bactericidal activity of blood thrombocytes in carps (Cyprinus carpio). Vet. Med. – Czech, 2002; 47: 21–25.
Tavares-Dias, M., Ono, E.A., Pilarski, F., Moraes, F.R.: Can thrombocytes participate in the removal of cellular debris in the blood circulation of teleost fish? A cytochemical study and ultrastructural analysis. J. Appl. Ichthyol., 2007; 23: 709-712.