The Effect of Proctodeal Gland Foam, and Depth and Frequency of Artificial Insemination on Fertility and Hatchability of Japanese Quail (Coturnix japonica)

The effect of the depth of semen deposition in the female reproductive tract, the addition of proctodeal gland foam to the net semen, and insemination frequency on fertility and hatchability of Japanese quails was investigated. In 2 experiments semen was collected from 30-32 males using the method of male stimulation by female. A group of 30-32 females was used in the fertility trials. In the first experiment semen obtained from group 1 was collected in empty glass tubes, while from group 2 it was collected in tubes containing Lake´s extender kept at 20 °C. Semen obtained from each group was deposited intravaginally or intrauterinely (4 groups of females were created, each comprising 8 specimens). In the second experiment, freshly collected semen was mixed with proctodeal gland foam and used for intravaginal insemination; semen from group 1 was inseminated 2x/week, while semen from group 2 was inseminated 3x/week. Keeping net (undiluted) semen or semen diluted with Lake´s extender for 15 min at room temperature reduced the number of live normal spermatozoa in relation to freshly collected semen. Egg fertility was higher after intravaginal insemination in comparison to intrauterine insemination (49.3% vs. 23.0% when undiluted semen was used, and 49.8% vs. 32.4 for semen diluted with Lake´s extender). Intravaginal insemination 3x/week with 20 ml of semen mixed 1:1 with proctodeal gland foam resulted in significantly higher (P < 0.01) egg fertility compared to insemination 2x/week (82.7% vs. 44.1%); the former was similar to that obtained in the naturally mated group (86.1%).

The Effect of Proctodeal Gland Foam, and Depth and Frequency of Artificial Insemination on Fertility and Hatchability of Japanese Quail (Coturnix japonica)

The effect of the depth of semen deposition in the female reproductive tract, the addition of proctodeal gland foam to the net semen, and insemination frequency on fertility and hatchability of Japanese quails was investigated. In 2 experiments semen was collected from 30-32 males using the method of male stimulation by female. A group of 30-32 females was used in the fertility trials. In the first experiment semen obtained from group 1 was collected in empty glass tubes, while from group 2 it was collected in tubes containing Lake´s extender kept at 20 °C. Semen obtained from each group was deposited intravaginally or intrauterinely (4 groups of females were created, each comprising 8 specimens). In the second experiment, freshly collected semen was mixed with proctodeal gland foam and used for intravaginal insemination; semen from group 1 was inseminated 2x/week, while semen from group 2 was inseminated 3x/week. Keeping net (undiluted) semen or semen diluted with Lake´s extender for 15 min at room temperature reduced the number of live normal spermatozoa in relation to freshly collected semen. Egg fertility was higher after intravaginal insemination in comparison to intrauterine insemination (49.3% vs. 23.0% when undiluted semen was used, and 49.8% vs. 32.4 for semen diluted with Lake´s extender). Intravaginal insemination 3x/week with 20 ml of semen mixed 1:1 with proctodeal gland foam resulted in significantly higher (P < 0.01) egg fertility compared to insemination 2x/week (82.7% vs. 44.1%); the former was similar to that obtained in the naturally mated group (86.1%).

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  • Van Drimmelen, G.C.: Artificial insemination of birds by the intraperitoneal route: a study in sex physiology of pigeons and fowls with reports upon a modified technique of semen collection, and a new technique of insemination, and observations on the spermatozoa in the genital organs of the fowl hen. Onderstepoort J. Vet. Res., 1951; 1 (suppl.): 1.
  • McFarquhar, A.M., Lake, P.E.: Artificial insemination in quail and the production of chicken-quail hybrids. J. Reprod. Fertil., 1964; 8: 261-263.
  • Marks, H.L., Lepore, P.D.: A procedure for artificial insemination of Japanese quail. Poult. Sci., 1965; 44: 1001-1003.
  • Chelmonska, B., Lukaszewicz, E., Kowalczyk, A., Jerysz, A.: The effect of proctodeal gland foam, diluent and dimethylacetamide addition on morphology and fertilising ability of Japanese quail (Coturnix japonica) spermatozoa. J. Poult. Sci., 2006; 43: 54-59.
  • Gwara, T., Walczak, M., Lukaszewicz, E., Chelmonska, B.: Effect of light source on characteristics of Japanese quail (Coturnix coturnix japonica) semen. Zesz. Nauk. AR we Wroclawiu, Zoot., 2004; 488: 103-109.
  • Lake, P.E.: Studies on the dilution and storage of fowl semen. J. Reprod. Fertil., 1960; 1: 30-35.
  • Fujihara, N., Nishiyama, H., Koga, O.: Physiological characteristics of quail (Coturnix coturnix japonica) spermatozoa. IVth Intern. Cong. of Andrology, Florence, Italy, 1989; 225-229.
  • Chelmonska, B., Jerysz, A., Wieliczko, A.: Morphological and bacteriological picture of Muscovy drakes semen. IIIthPolish- Czechoslovakian Symp. on Avian Physiology, Cracow, Poland, 1989; 18.
  • Lukaszewicz, E.: Study of the diluents for cock’s semen storage in the light of laboratory estimation and fertility rates. Zesz. Nauk. AR we Wroclawiu, Zoot., 1988; 168: 43-59.
  • Lukaszewicz, E.: Cryopreservation of Anser anser L. gander semen. Zesz. Nauk. AR we Wroclawiu, 2002; 440: 1-111.
  • Sontakke, S.D., Umapathy, G., Sivaram, V., Kholkute, S.D., Shivaji, S.: Semen characteristics, cryopreservation, and successful artificial insemination in the Blue rock pigeon (Columba livia). Theriogenology, 2004; 62: 139-153.
  • Amano, T., Watanabe, S.: Studies on artificial insemination in Japanese quail. 1. On a method of semen collection and evaluation of semen. J. Agric. Sci., 1966; 12: 59-62.
  • Kobayashi, S., Okamoto, S., Matsuo, T.: The influence of the foamy materials on the fertilising capacity of semen in the Japanese quail (Coturnix coturnix japonica). Agric. Bull. Sage University, 1972; 32: 89-95.
  • Ogawa, K., Nakanishi, Y., Tojo, H., Imanishi, M.: Effect of frothy fluid from cloacal gland on fertility in the Japanese quail (Coturnix coturnix japonica). Bull. Fac. Agric. Kagoshima Univ., 1974; 24: 35-40.
  • Cheng, K.M., McIntyre, R.F., Hickman, A.R.: Proctodeal gland foam enhances competitive fertilisation in domestic Japanese quail. Auk, 1989; 106: 286-291.
  • Surai, P.F., Wishart, G.J.: Poultry artificial insemination technology in the countries of the former USSR. World’s Poult. Sci. J., 1996; 52: 27-43.
  • Lepore, P.D., Marks, H.L.: Intravaginal insemination of Japanese quail: factors influencing the basic technique. Poult. Sci., 1966; 45: 888-891.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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