The cSNP scanning and expression analysis of the duck FTH1 gene
Ferritin, heavy polypeptide 1 (FTH1) is a kind of ferritin complex that catalyzes the conversion of Fe(II) into Fe(III) to protect the cell from oxidative damage. Recent research has shown that FTH1 is also associated with several disease-related processes, such as inflammation and tumor progression and so on. In this study, the coding sequence (CDS) of FTH1 in Jinding ducks was cloned. The CDS sequence revealed an open reading frame of 546 nucleotides encoding a protein with 181 amino acids. Based on this sequence, the duck FTH1 protein is predicted to have conserved domains typical of eukaryotic ferritin. Only one synonymous mutation (c.447T > C) of the coding sequence of the duck FTH1 gene was identified in ducks. Quantitative RT-PCR results revealed that FTH1 expression in the liver and spleen was significantly downregulated after duck hepatitis virus 1 infection. Meanwhile, in DF-1 cells transfected with an FTH1 plasmid, expression of the antiviral marker gene Mx1 was significantly upregulated. These results suggest that the coding sequence of the duck FTH1 gene was highly conserved and FTH1 plays an important role in antivirus activity.
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- Pietrangelo A. The ferroportin disease. Blood Cells Mol Dis
2004; 32: 131-138.
- Fernandez-Real JM, Ricart-Engel W, Arroyo E, Balanca R,
Casamitjana-Abella R, Cabrero D, Fernandez-Castaner M,
Soler J. Serum ferritin as a component of the insulin resistance
syndrome. Diabetes Care 1998; 21: 62-68.
- Gozzelino R, Soares MP. coupling heme and iron metabolism
via ferritin H chain. Antioxid Redox Sign 2014; 20: 1754-1769.
- Hofer H, Osterreicher C, Jessner W, Penz M, Steindl-Mundal
P, Wrba F, Ferenci P. Hepatic iron concentration does not
predict response to standard and pegylated-IFN/ribavirin
therapy in patients with chronic hepatitis C. J Hepatol 2004;
40: 1018-1022.
- Yamanishi H, Iyama S, Yamaguchi Y, Kanakura Y, Iwatani Y.
Relation between iron content of serum ferritin and clinical
status factors extracted by factor analysis in patients with
hyperferritinemia. Clin Biochem 2002; 35: 523-529.
- Nielsen P, Engelhardt R, Dullmann J, Fischer R. Non-invasive
liver iron quantification by SQUID-biosusceptometry and
serum ferritin iron as new diagnostic parameters in hereditary
hemochromatosis. Blood Cell Mol Dis 2002; 29: 451-458.
- Ricolleau G, Charbonnel C, Lode L, Loussouarn D, Joalland
MP, Bogumil R, Jourdain S, Minvielle S, Campone M, Deporte-
Fety R et al. Surface-enhanced laser desorption/ionization
time of flight mass spectrometry protein profiling identifies
ubiquitin and ferritin light chain as prognostic biomarkers
in node-negative breast cancer tumors. Proteomics 2006; 6:
1963-1975.
- Costanzo F, Colombo M, Staempfli S, Santoro C, Marone
M, Frank R, Delius H, Cortese R. Structure of gene and
pseudogenes of human apoferritin H. Nucleic Acids Res 1986;
14: 721-736.
- Levi S, Luzzago A, Cesareni G, Cozzi A, Franceschinelli F,
Albertini A, Arosio P. Mechanism of ferritin iron uptake:
activity of the H-chain and deletion mapping of the ferro-
oxidase site. A study of iron uptake and ferro-oxidase activity
of human liver, recombinant H-chain ferritins, and of two
H-chain deletion mutants. J Biol Chem 1988; 263: 18086-
18092.
- Harrison PM, Arosio P. The ferritins: molecular properties,
iron storage function and cellular regulation. BBA-
Bioenergetics 1996; 1275: 161-203.
- Storr HL, Kind B, Parfitt DA, Chapple JP, Lorenz M, Koehler
K, Huebner A, Clark AJL. Deficiency of ferritin heavy-chain
nuclear import in triple A syndrome implies nuclear oxidative
damage as the primary disease mechanism. Mol Endocrinol
2009; 23: 2086-2094.
- Coffman LG, Parsonage D, D’Agostino R Jr, Torti FM, Torti SV.
Regulatory effects of ferritin on angiogenesis. P Natl Acad Sci
USA 2009; 106: 570-575.
- Li R, Luo C, Mines M, Zhang J, Fan GH. Chemokine CXCL12
induces binding of ferritin heavy chain to the chemokine
receptor CXCR4, alters CXCR4 signaling, and induces
phosphorylation and nuclear translocation of ferritin heavy
chain. J Biol Chem 2006; 281:37616-37627.
- Li X, Xu Q, Zhang Y, Bi YL, Zhao RX, Chen CY, Duan XJ, Chen
GH. Construction of a suppression subtractive hybridization
cDNA library to screen differentially expressed genes from
duck liver infected duck hepatitis virus. Acta Vet Zootechnica
Sinica 2012; 43: 211-219.
- Nair S, Arathy DS, Issac A, Sreekumar E. Differential gene
expression analysis of
in vitro
duck hepatitis B virus infected
primary duck hepatocyte cultures. Virol J 2011; 8: 363-374.
- Jiang Z, Applegate TJ, Lossie AC. Cloning, annotation and
developmental expression of the chicken intestinal
MUC2
gene. PLoS One 2013; 8: e53781.
- Toth TE. Studies of an agent causing mortality among
ducklings immune to duck virus hepatitis. Avian Dis 1969; 13:
834-846.
- Jin X, Zhang W, Zhang W, Gu C, Cheng G, Hu X. Identification
and molecular analysis of the highly pathogenic duck hepatitis
virus type 1 in Hubei province of China. Res Vet Sci 2008; 85:
595-598.
- Tseng CH, Knowles NJ, Tsai HJ. Molecular analysis of duck
hepatitis virus type 1 indicates that it should be assigned to a
new genus. Virus Res 2007; 123: 190-203.
- Arosio P, Ingrassia R, Cavadini P. Ferritins: a family of
molecules for iron storage, antioxidation and more. BBA-Gen
Subjects 2009; 1790: 589-599.
- Bou-Abdallah F. The iron redox and hydrolysis chemistry of
the ferritins. BBA-Gen Subjects 2010; 1800: 719-731.
- Chasteen ND. Ferritin. Uptake, storage, and release of iron.
Metal Ions Biol Syst 1998; 35: 479-514.
- Harrison PM, Arosio P. The ferritins: molecular properties,
iron storage function and cellular regulation. BBA-
Bioenergetics 1996; 1275: 161-203.
- Theil EC. The ferritin family of iron storage proteins. Adv
Enzymol Relat Areas Mol Biol 1990; 63: 421-449.
- Lee G, Han D, Song JY, Kim JH, Yoon S. Proteomic analysis
of swine hepatitis E virus (sHEV)-infected livers reveals
upregulation of apolipoprotein and downregulation of ferritin
heavy chain. FEMS Immunol Med Mic 2011; 61: 359-363.
- Dillon D, Runstadler J. Mx gene diversity and influenza
association among five wild dabbling duck species (
Anas
spp.)
in Alaska. Infect Genet Evol 2010; 10: 1085-1093.
- Samuel CE. Antiviral actions of interferons. Clin Microbiol
Rev 2001; 14: 778-809.
- Fernandez-Trujillo MA, Garcia-Rosado E, Alonso MC, Castro
D, Alvarez MC, Bejar J. Mx1, Mx2 and Mx3 proteins from
the gilthead seabream (
Sparus aurata
) show
in vitro
antiviral
activity against RNA and DNA viruses. Mol Immunol 2013;
56: 630-636.
- Haller O, Kochs G, Weber F. Interferon, Mx, and viral
countermeasures. Cytokine Growth F R 2007; 18: 425-433