Cenker Çağrı CINGI, Abdurrahman Fatih FİDAN, Durmuş Fatih BAŞER, Mehmet UÇAR, Zülfükar Kadir SARITAŞ

Stress response related to ultrasonographic examination in dogs

This study investigated whether abdominal ultrasonography, a frequently used examination method in clinics and hospitals, causes stress in dogs. For the study, 10 healthy dogs were used. Following general examination, intravenous catheters were placed in the V. cephalica accecorius of the dogs and blood specimens were collected to obtain serum and plasma samples (minute 0). After a 30- min break, ultrasonography was applied following shaving of the abdominal region. Serum and plasma samples were collected from the intravenous catheters at the 5th, 15th, and 20th minutes of the ultrasonographic examination during which the urinary bladder, left kidney, spleen, stomach, liver, right kidney, transverse colon, and ascending colon were examined. The total antioxidant status (TAS), total oxidant status (TOS), nitric oxide (NOx), and cortisol levels of serum and plasma samples were analyzed and differences were statistically determined with respect to time. No statistically significant time-dependent changes in the TAS and cortisol values were observed. However, there was a time-dependent change in the TOS and NOx values, but when comparing all the times, no changes were observed. The results showed that abdominal ultrasonographic examination, a routinely and frequently used method, does not induce any stress factors and therefore cannot lead to health problems.

Keywords:

Dog, ultrasonography, oxidative stress,

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1. Savaş T, Yurtman IY, Tölü C. Animal rights and animal welfare: philosophical view objective pursuit. Hayvansal Üretim 2009; 50 (1): 54-61 (in Turkish with an abstract in English).
2. Kara NK, Koyuncu M. Organic dairy farming and welfare. Uludağ Üniversitesi Ziraat Fakültesi Dergisi 2011; 25 (1): 165- 173 (in Turkish with an abstract in English).
3. Avci G, Küçükkurt I, Fidan AF, Eryavuz A, Aslan R et al. The influence of vitamin C and xylazine on cortisol, lipid peroxidation and some biochemical parameters in transported sheep. Fırat University Journal of Health Science (Vet) 2008; 22 (3): 147-152.
4. Dantzer R, Mormède P. Stress in farm animals: A need for a reevaluation. Journal of Animal Science 1983; 57 (1): 6-18.
5. Freeman LJ, Nixon PGF, Sallabank P, Reaveley D. Psychological stress and silent myocardial ischemia. American Heart Journal 1987; 114 (3): 477-482.
6. Fidan AF, Pamuk K, Ozdemir A, Saritas ZK, Tarakci U. Effects of dehorning by amputation on oxidant-antioxidant status in mature cattle. Revue de Medecine Veterinaire 2010; 161 (11): 502-508. doi: 10.12681/jhvms.15544
7. Mattoon JS, Nyland TG. Ultrasonography of the general abdomen. In: Nyland TG and Mattoon JS (editors). Veterinary Diagnostic Ultrasound. 1st ed. Philadelphia, PA, USA: WB Saunders Company; 1995. pp. 43-51.
8. Şındak N, Biricik HS. Ultrasonographic evaluation of intraabdominal organ diseases in dogs. Yüzüncü Yıl Üniversitesi Veterinerlik Fakültesi Dergisi 2006; 17 (1-2): 75-79 (in Turkish with an abstract in English).
9. Miranda KM, Espey MG, Wink AD. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5 (1): 62-71. doi: 10.1006/ niox.2000.0319
10. Ghiselli A, Serafini M, Natella F, Scaccini C. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data. Free Radical Biology and Medicine 2000; 29 (11): 1106-1114. doi: 10.1016/S0891-5849(00)00394-4
11. Kayar A, Dokuzeylul B, Kandemir FM, Kirbas A, Bayrakal A et al. Total oxidant and antioxidant capacities, nitric oxide and malondialdehyde levels in cats seropositive for the feline coronavirus. Veterinarni Medicina 2015; 60 (5): 274-281. doi: 10.17221/8180
12. Almeida BFM, Narciso LG, Melo LM, Preve PP, Bosco AM et al. Leishmaniasis causes oxidative stress and alteration of oxidative metabolism and viability of neutrophils in dogs. The Veterinary Journal 2013; 198 (3): 599-605. doi: 10.1016/j. tvjl.2013.08.024
13. Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 2005; 38 (12): 1103- 1111. doi: 10.1016/j.clinbiochem.2005.08.008
14. Plavec T, Nemec Svete A, Butinar J, Tozon N, Prezelj M et al. Antioxidant status in canine cancer patients. Acta Veterinaria 2008; 58 (2/3): 275-286. doi: 10.2298/AVB0803275P
15. Kogika MM, Lustoza MD, Hagiwara MK, Caragelasco DS, Martorelli CR et al. Evaluation of oxidative stress in the anemia of dogs with chronic kidney disease. Veterinary Clinical Pathology 2015; 44 (1): 70-78. doi: 10.1111/vcp.12225
16. Chaudhuri S, Varshney JP, Patra RC. Erythrocytic antioxidant defense, lipid peroxides level and blood iron, zinc and copper concentrations in dogs naturally infected with Babesia gibsoni. Research in Veterinary Science 2008; 85 (1): 120-124. doi: 10.1016/j.rvsc.2007.09.001
17. Olvera JRL. Capture, restraint and transport stress in Southern chamois (Rupicapra pyrenaica) modulation with acepromazine and evaluation using physiological parameters. PhD, Universitat Autònoma de Barcelona, Barcelona, Spain, 2004.
18. Cingi CC, Baser DF, Karafakioglu YS, Fidan AF. Stress response in dairy cows related to rectal examination. Acta Scientiae Veterinariae 2012; 40 (3): 1053.
19. Ortiz-de-Montellano M, Galindo-Maldonado F, CavazosArizpe EO, Aguayo-Arceo AM, Torres-Acosta JFJ et al. Effect of electro-ejaculation on the serum cortisol response of Criollo goats (Capra hircus). Small Ruminant Research 2007; 69: 228- 231. doi: 10.1016/j.smallrumres.2005.12.025
20. Morgan KN, Tromborg CT. Sources of stress in captivity. Applied Animal Behaviour Science 2007; 102: 262-302. doi: 10.1016/j.applanim.2006.05.032
21. Poole T. Happy animals make good science. Laboratory Animals 1997; 31 (2): 116-124. doi: 10.1258/002367797780600198
22. Beerda B, Schilder MBH, Bernadina W, Van Hooff JARAM, De Vries HW et al. Chronic stress in dogs subjected to social and spatial restriction. II. Hormonal and immunological responses. Physiology & Behavior 1999; 66 (2): 243-254. doi: 10.1016/ S0031-9384(98)00290-X
23. Tabakoğlu E, Durgut R. Veteriner hekimlikte oksidatif stres ve bazı önemli hastalıklarda oksidatif stresin etkileri. AVKAE Dergisi 2013; 3 (1): 69-75 (in Turkish).
24. Rahal A, Kumar A, Singh V, Yadav B, Tiwari R et al. Oxidative stress, prooxidants, and antioxidants: The interplay. BioMed Research International 2014; 2014: 761264. doi: 10.1155/2014/761264
25. Bohloli M, Uzun H, Aytac E, Toklu AS, Paksoy M et al. Hyperbaric oxygen (HBO) therapy after partial hepatectomy: an experimental study on oxidative stress in rats. Scandinavian Journal of Laboratory Animal Science 2007; 34: 131-140. doi: 10.1620/tjem.203.253
26. Rijnberk AD, Mol JA. Adrenocortical function. In: Kaneko JJ, Harvey JW, Bruss ML (editors). Clinical Biochemistry of Domestic Animals. 5th ed. San Diego, CA, USA: Academic Press; 1997. pp. 553-570.