Rotifer, Brachionus plicatilis, üretiminde küçük-ölçekli, yüksek-yoğunluklu, sürekli sistem performansı
Bu çalışmada rotifer Brachionus plicatilis, üretiminde kullanılan geleneksel, düşük yoğunlukta kesikli-kültür sistemlerine bir alternatif olarak, yüksek yoğunluklu, sürekli-kültür sisteminin performansı değerlendirilmiştir. On ile onsekiz gün arasında değişen 4 farklı denemede 1700-3760 rotifer/mL yoğunluğa ulaşılmıştır. Amonyak ve pH’a bağlı sorunlar, yüksek oranlarda su değişimi ve periyodik amonyak giderici kullanılarak kontrol altında tutulmuştur. NH3-N ve pH değerleri, sırasıyla 0-8,8 mg/L ve 6,9-9,3 değişirken, en önemli sorun 85 mL/L seviyesine kadar ulaşan toplam çökebilen katı maddelerin birikimi olmuştur. Katı madde kontrolünü etkin bir biçimde azaltacak basit ve düşük maliyetli filtrelerin geliştirilmesi için çalışmalar gereklidir. Konsantre alg ile sürekli sistemlerin birlikte kullanımı mevcut kesiklikültür sistemlerine göre etkin bir alternatif olabilir
The performance of a small-scale, high-density, continuous system for culturing the rotifer Brachionus plicatilis
The performance of a high-density continuous system was evaluated as an alternative to conventional low-density batch-culture systems for culturing the rotifer Brachionus plicatilis. Rotifer densities ranging from 1700 to 3760 individuals/mL were achieved in 4 different trials that lasted 10-18 days. Problems associated with NH3-N and pH were controlled by increasing the water exchange rate and periodic addition of ammonia remover. While NH3-N and pH values were 0-8.8 mg/L and 6.9-9.3, respectively, the most important problem was the accumulation of TSS at concentrations of up to 85 mL/L within a very short time after start-up. Further studies are required to develop simple and cost-effective filters that reduce TSS concentrations. Use of continuous systems in combination with concentrated algae is potentially an effective alternative to existing batch-culture systems.
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- Dhert, P., Rombaut, G., Suantika, G., Sorgeloos, P.: Advancement of rotifer culture and manipulation techniques in Europe. Aquaculture, 2001; 200: 129-146.
- Hagiwara, A., Gallardo, W.G., Assavaaree, M., Kotani, T., Araujo, A.B.: Live food production in Japan: recent progress and future aspects. Aquaculture, 2001; 200: 111-127. 6. Yoshimura, K., Tanaka, K., Yoshimatsu, T.: A novel culture system for the ultra-high density production of the rotifer, Brachionus rotundiformis - a preliminary report. Aquaculture, 2003; 227: 165-172.
- Fu, Y., Hada, A., Yamashita, T., Yoshida, Y., Hino, A.: Development of a continuous culture system for stable mass production of the marine rotifer Brachionus. Hydrobiologia, 1997; 358: 145-151. 8. Greenberg, A.E., Trussell, R.R., Clesceri, L.S.: Standard Methods for the Examination of Water and Wastewater. American Public Health Association, Washington. 1985; 98-99. 9. Øie, G., Reitan, K.I., Olsen, Y.: Comparison of rotifer culture quality with yeast plus oil and algal-based cultivation diets. Aquacult. Int., 1994; 2: 225-238.
- Bentley, C.D., Carroll, P.M., Watanabe, W.O., Riedel, A.M.: Intensive rotifer production in a pilot-scale continuous culture recirculating system using nonviable microalgae and an ammonia neutralizer. J. World Aquacult. Soc., 2008; 39: 625- 635.
- Riche, M., Pfeiffer, T.J., Garcia, J.: Evaluation of a sodium hydroxymethanesulfonate product for reducing total ammonia nitrogen in a small-scale rotifer batch culture system. N. Am. J. Aquacult., 2006; 68: 199-205.
- Yoshimura, K., Hagiwara, A., Yoshimatsu, T., Kitajima, C.: Culture technology of marine rotifers and the implications for intensive culture of marine fish in Japan. Mar. Freshwater Res., 1996; 47: 217-222.
- Yoshimura, K., Usuki, K., Yoshimatsu, T., Kitajima, C., Hagiwara, A.: Recent development of a high density mass culture system for the rotifer Brachionus rotundiformis Tschugunoff. Hydrobiologia, 1997; 358: 139-144.
- Yu, J.P., Hirayama, K.: The effect of un-ionized ammonia on the population growth of the rotifer in mass culture. B. Jpn. Soc. Sci. Fish., 1986; 52: 1509-1513.