Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep

Öz DIO2 was studied as a candidate gene for reproduction in sheep. Three pairs of primers were designed to detect single nucleotide polymorphisms (SNPs) of exon 2 of DIO2 in nonseasonal and seasonal reproduction breeds. The polymorphisms were detected using SeqMan II and analyzed by PCR-RFLP. The sheep DIO2 gene was cloned and its mutations were detected in five sheep breeds whose reproductive seasonality and litter sizes were different. Association analyses were performed to reveal the relationships between SNPs and reproductive seasonality as well as litter size in sheep. The results showed that a 1041-bp DNA fragment of the sheep DIO2 gene was obtained and a part of the coding sequence of 591 bp encodes 196 amino acids, having high homology with that of other mammals. Moreover, three mutations (G318T, G482C, and C885T) were detected. The test of genotype distribution in five sheep breeds showed that there were significant differences between nonseasonal and seasonal breeds for 318 and 482 loci. However, the associations between the three polymorphisms and sheep seasonal reproduction were not very distinct. Additionally, there was no significant associations between polymorphisms and litter sizes in Small Tail Han sheep. These results may provide the basic theory for further study.

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Bibtex @ { tbtkveterinary577105, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2016}, volume = {40}, pages = {142 - 149}, doi = {}, title = {Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep}, key = {cite}, author = {He, Jianning and Huang, Dongwei and Dı, Ran and Wang, Jinxin and Chu, Mingxing and Lıu, Qiuyue and Hu, Wenping and Wang, Xiangyu and Pan, Zhangyuan} }
APA He, J , Huang, D , Dı, R , Wang, J , Chu, M , Lıu, Q , Hu, W , Wang, X , Pan, Z . (2016). Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep . Turkish Journal of Veterinary and Animal Sciences , 40 (2) , 142-149 .
MLA He, J , Huang, D , Dı, R , Wang, J , Chu, M , Lıu, Q , Hu, W , Wang, X , Pan, Z . "Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep" . Turkish Journal of Veterinary and Animal Sciences 40 (2016 ): 142-149 <
Chicago He, J , Huang, D , Dı, R , Wang, J , Chu, M , Lıu, Q , Hu, W , Wang, X , Pan, Z . "Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep". Turkish Journal of Veterinary and Animal Sciences 40 (2016 ): 142-149
RIS TY - JOUR T1 - Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep AU - Jianning He , Dongwei Huang , Ran Dı , Jinxin Wang , Mingxing Chu , Qiuyue Lıu , Wenping Hu , Xiangyu Wang , Zhangyuan Pan Y1 - 2016 PY - 2016 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 142 EP - 149 VL - 40 IS - 2 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep %A Jianning He , Dongwei Huang , Ran Dı , Jinxin Wang , Mingxing Chu , Qiuyue Lıu , Wenping Hu , Xiangyu Wang , Zhangyuan Pan %T Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep %D 2016 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 40 %N 2 %R %U
ISNAD He, Jianning , Huang, Dongwei , Dı, Ran , Wang, Jinxin , Chu, Mingxing , Lıu, Qiuyue , Hu, Wenping , Wang, Xiangyu , Pan, Zhangyuan . "Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep". Turkish Journal of Veterinary and Animal Sciences 40 / 2 (Nisan 2016): 142-149 .
AMA He J , Huang D , Dı R , Wang J , Chu M , Lıu Q , Hu W , Wang X , Pan Z . Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep. Turkish Journal of Veterinary and Animal Sciences. 2016; 40(2): 142-149.
Vancouver He J , Huang D , Dı R , Wang J , Chu M , Lıu Q , Hu W , Wang X , Pan Z . Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep. Turkish Journal of Veterinary and Animal Sciences. 2016; 40(2): 142-149.
IEEE J. He , D. Huang , R. Dı , J. Wang , M. Chu , Q. Lıu , W. Hu , X. Wang ve Z. Pan , "Polymorphism of exon 2 of DIO2 gene and its association withseasonal reproduction in sheep", Turkish Journal of Veterinary and Animal Sciences, c. 40, sayı. 2, ss. 142-149, Nis. 2016
Turkish Journal of Veterinary and Animal Sciences
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

61.6b55.9b

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