Babu Lal JANGIR, Raj Bahadur BIND, Pawan KUMAR, Ramesh SOMVANSHI

Pathological studies and detection of different bovine papilloma virus types in buffalo cutaneous warts

Bovine papillomatosis is a common viral disease of cattle but is relatively less common in buffaloes. Bovine papilloma virus (BPV) -1 and -2 are mostly associated with cutaneous warts in buffaloes. The aim of the present work was to study the pathomorphology and association of different BPV types in buffalo cutaneous warts. A total of 10 cases were included in the present study, which revealed that growths were either single or multiple, variable in shape and size, and present at different body sites. Histopathologically, these were categorized as papilloma, fibropapilloma, and fibroma. Polymerase chain reaction (PCR) revealed the presence of BPV-1, -2, and -5 DNA. Quantitative real-time PCR showed varying amounts of viral DNA copy numbers. On immunohistochemistry, nuclear Ki-67 expression was observed mainly in the cells of suprabasal layers. In conclusion, DNA of BPV-1, -2, and -5 was detected either alone or their mixed infections. Nuclear Ki-67 immunoreactivity in the suprabasal layer indicated that this is the cellular proliferation site.

Keywords:

Bovine papilloma virus, buffalo, cutaneous warts, Ki-67,

PDF

___

Singh V, Somvanshi, R. BPV-2 associated papillomatosis in Indian water buffaloes. Indian J Anim Sci 2010; 80: 956-960.
Somvanshi R. Papillomatosis in buffaloes: a less-known disease. Trans Emerg Dis 2011; 58: 327-332.
Silvestre O, Borzacchiello G, Nava D, Iovane G, Russo V, Vecchio D, D’Ausilio F, Gault EA, Campo MS, Paciello O. Bovine papilloma virus type 1 DNA and E5 oncoprotein expression in water buffalo fibropapillomas. Vet Pathol 2009; 46: 636-641.
Doorbar J. The papilloma virus life cycle. J Clinc Virol 2005; 32S: 7S-15S.
Bernard HU, Burk RD, Chen Z, Van Doorslaer K, Zur HH, De Villiers EM. Classification of papilloma viruses based on 189 PV types and proposal of taxonomic amendments. Virology 2010; 401: 70-79.
Hatama S, Ishihara R, Ueda Y, Kanno T, Uchida I. Detection of a novel bovine papillomavirus type 11 (BPV-11) using xipapilloma virus consensus polymerase chain reaction primers. Arch Virol 2011; 156: 1281-1285.
Zhu W, Dong J, Shimizu E, Hatama S, Kadota K, Goto Y, Haga T. Characterization of novel bovine papilloma virus type 12 (BPV-12) causing epithelial papilloma. Arch Virol 2012; 157: 85-91.
Lunardi M, Alfieri AM, Otonel A, Rodrigues WB, De Miranda AB, Alfieri AF. Genetic characterization of a novel bovine papillomavirus member of the Deltapapillomavirus genus. Vet Microbiol 2013; 162: 207-213.
Lu S, Tiekso J, Hietanen S, Syrjaenen K, Havu VK, Syrjaenen S. Expression of cell-cycle proteins p53, p21 (WAF-1), PCNA and Ki-67 in benign, premalignant and malignant skin lesions with implicated HPV involvement. Acta Dermato Venereologica 1999; 79: 268-273.
Yaguiu A, de Carvalho C, de Freitas AC, Stocco DS. Papillomatosis in cattle: in situ detection of bovine papillomavirus DNA sequences in reproductive tissues. Brazilian J Morphol Sci 2006; 23: 525-529.
Aaltomaa S, Lipponen P, Papinaho S, Syrjanen K. Proliferating- cell nuclear antigen (PC10) immunolabelling and other proliferation indices as prognostic factors in breast cancer. J Cancer Res Clin Oncol 1993; 119: 288-294.
Jelinek F, Tachezy R. Cutaneous papillomatosis in cattle. J Comp Pathol 2005; 132: 70-81.
Kumar P, Jangir BL, Saikumar G, Somvanshi R. Co-infection of bovine papillo-mavirus type-1 and -10 in teat warts of dairy cattle. Vet Med 2013; 58: 605-608.
Pangty K, Singh S, Saikumar G, Goswami R, Somvanshi R. Detection of BPV-1 and -2 and quantification of BPV-1 by real-time PCR in cutaneous warts in cattle and buffaloes. Trans Emerg Dis 2010; 57: 185-196.
Kumar P, Nagarajan N, Kumar D, Bind RB, Somvanshi R. Detection and quantification of Bovine Papillomaviruses (BPVs) in cutaneous warts of cattle and buffaloes. Adv Anim Vet Sci 2013; 1: 53-58.
Campo MS, Jarrett WF, O’Neil V, Barron RJ. Latent papillomavirus infection in cattle. Res Vet Sci 1994; 56: 151- 157.
Campo MS. Animal model of papillomavirus pathogenesis. Virus Res 2002; 89: 249-261.
Kumar P, Nagarajan N, Saikumar G, Arya RS, Somvanshi R. Detection of bovine papilloma viruses in wart-like lesions of upper gastrointestinal tract of cattle and buffaloes. Trans Emerg Dis 2015; 62: 264-271.
Carvalho CCR, Batista MVA, Silva MAR, Balbino VQ, Freitas AC. Detection of bovine papillomavirus types, co-infection and a putative new BPV 11 subtype in cattle. Trans Emerg Dis 2012; 59: 441-447.
Boon ME, Howard CV, Velzen D. PCNA independence of Ki67 expression in HPV infection. Cell Biol Int 1993; 17: 1001-1004.