Ovarian Follicle Ultrastructure and Changes in Levels of Ovarian Steroids during Oogenesis in Chalcalburnus tarichi Pallas, 1811

The ultrastructure of the ovarian follicle and the levels of the ovarian 11-dehydrocorticosterone (11-DHC), estradiol-17b (E2), 17a-hydroxyprogesterone (17a-OHP), and progesterone (P) were studied during cortical alveoli, vitellogenesis and oocyte maturation in Chalcalburnus tarichi. The microvilli began to form on the oocyte surface during cortical alveoli phase and during vitellogenesis, came into contact in the pore canals of the zona radiata with microvilli formed from granulosa cells. While the special thecal cells posses specific organelles, which are characteristic of steroid-producing cells, namely, mitochondria with tubular cristae, smooth endoplasmic reticulum and lipid droplets, the granulosa cells contain organelles typical for protein-secreting cells. These findings suggest that during vitellogenesis the special thecal cells are the sites of steroid synthesis in the C. tarichi ovary. The levels of 11-DHC and progesterone were measured at lower concentration than other hormones. While there was a decline in 11-DHC level during cortical alveoli phase (from 52.60 ± 6.54 to 20.33 ± 6.74 ng/ml), during vitellogenesis it increased gradually and reached a peak at the end of vitellogenesis (68.90 ± 9.99 ng/ml). At the beginning of maturation phase, it decreased to baseline level (22.90 ± 1.87 ng/ml) and again increased at the end of the maturation (54 ± 0.75 ng/ml). E2 levels started to increase at cortical alveoli phase and this increase continued during vitellogenesis too. In February, a small decline was noted. However, at the end of vitellogenesis the level of E2 reached its maximum value (756.10 ± 26.5 ng/ml). At the maturation phase (in April) it decreased to 213.50 ± 25.5 ng/ml and again a slight increase was perceived (in May). 17a-OHP level was 100.10 ± 14.4 ng/ml at the beginning of the cortical alveoli phase and it decreased to 13.40 ± 1.3 ng/ml at the end of the phase. It started to increase during vitellogenesis and reached its maximum value at the end of vitellogenesis (213.90 ± 8.14 ng/ml). At the beginning of the maturation phase (in April) an evident decline was noted (68.00 ± 8.58 ng/ml). It increased again to 142.20 ± 4.45 ng/ml at the end of maturation (in May). P level was measured to 50.90 ± 2.37 ng/ml at the beginning of the cortical alveoli phase (in August), and it decreased to 22.80 ± 0.50 ng/ml at the end of the phase. This low value was kept at the beginning of vitellogenesis, but during vitellogenesis a small increase was noted and at the end of the phase it was measured as 50.80 ± 4.03 ng/ml. This low level is kept during maturation phase too. These results suggest that in C. tarichi none of these hormones (11-DHC, E2, 17a- OHP and P) is effective during the cortical alveoli phase, E2, 11-DHC and 17a- OHP are effective during vitellogenesis while P has no effect and 11-DHC and 17a- OHP are effective at the end of maturation while E2 and P have no role.

Anahtar Kelimeler:

Chalcalburnus tarichi, ovarian follicles, ovarian steroids, ultrastructure

Ovarian Follicle Ultrastructure and Changes in Levels of Ovarian Steroids during Oogenesis in Chalcalburnus tarichi Pallas, 1811

Keywords:

Chalcalburnus tarichi, ovarian follicles, ovarian steroids, ultrastructure,

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Kagawa, H., Young, G., Nagahama, Y.: Relationship between seasonal plasma estradiol-17βand testosterone levels and in vitro production by ovarian follicles of Amago salmon (Oncorhynchus rhodurus) Biol. Reprod., 1983; 29: 301-309.
Kagawa, H., Young, G., Nagahama, Y.: In vitro estradiol-17βand testosterone production by ovarian follicles of the Goldfish, Carassius auratus. Gen. Comp. Endocrinol., 1984; 54: 139-143.
Shimizu, A., Aida, K., Hanyu, I.: Endocrine profiles during the short reproductive cycle of an autumn-spawning bitterling, Acheilognathus rhombea. Gen. Comp. Endocrinol., 1985; 60: 361-371.
Sakai, N., Iwamatsu, T., Yamauchi, K., Suzuki, N., Nagahama, Y.: Influence of follicular development on steroid production in the medaka (Oryzias latipes) ovarian follicle in response to exogenous substrates. Gen. Comp. Endocrinol., 1988; 71: 516-523.
Venkatesh, B., Tan, C.H., Lam, T.J.: Blood steroid levels in the Goldfish: Measurement of six ovarian steroids in small volumes of serum by reverse-phase high-performance liquid chromatography and radioimmunoassay. Gen. Comp. Endocrinol., 1989; 76, 398- 407.
Sundararaj, B.I., Nath, P.: Synthesis of vitellogenin and its uptake by the ovary in the catfish, Heteropneustes fossilis (Bloch). Gen. Comp. Endocrinol., 1981; 43: 201-210.
Sundararaj, B.I., Nath, P., Burzava-Gerard, E.: Synthesis of vitellogenin and its uptake by the ovary in the catfish, Heteropneustes fossilis (Bloch) in response to carp gonadotropin and subunits. Gen. Comp. Endocrinol., 1982; 46: 93-98.
Pankhurst, N.W., Stacey, N.E., Van Der Kraak, G.: Reproductive development and plasma levels of reproductive hormones of goldeye, Hiodon alosoides (Rafinesque), taken from the North Saskatchewan river during the open-water season. Can. J. Zool., 1986; 64: 2843-2849.
Goetz, W.F. Bergman, H.L.: The effects of steroids in final maturation and ovulation of oocytes from brook trout (Salvelinus fontinalis) and yellow perch (Perca flavescens). Biol. Reprod., 1978; 18: 293-298.
Goswami, S.V., Sundararaj, B.I.: Temporal effects of ovine luteinizing hormones and desoxycorticosterone acetate on maturation and ovulation of oocytes of the Catfish, Heteropneustes fossilis (Blonch): An in vitro and in vivo study. J. Exp. Zool., 1971; 178: 457-466.
Pankhurst, N.W., Conroy, A.M.: Endocrine change during gonadal maturation and spawning in the orange roughy (Hoplostethus atlanticus Collett) a teleost from the Midslope waters of New Zealand. Gen. Comp. Endocrinol., 1988; 70: 262-273.
Battal, P., Ünal, G.: Hayvansal hormonların izokratik yüksek basınçlı sıvı kromatografisi (HPLC) yöntemiyle belirlenmesi. II. Ulusal Kromatografi Kongresi Kitapcığı 2001; 138-145.
Takashima, F., Hibiya, T.: An Atlas of Fish Histology. Gustav Fischer Verlag, 1995; 129-150.
Selman, K., Wallace, R.A.: Gametogenesis in Fundulus heteroclitus. Am. Zool., 1986; 26: 173-192.
Hamazaki, T., Iuchi, I., Yamagami, K.: Production of a “spawning female-specific substance” in hepatic cells and its accumulation in the ascites of the estrogen-treated adult fish, Orizias latipes. J, Exp. Zool., 1987; 242: 325-332.
Hamazaki, T., Iuchi, I., Yamagami, K.: Isolation and partial characterization of a “spawning female-specific substance” in the teleost, Orizias latipes. J. Exp. Zool., 1987; 242: 343-349.
Matsuyama, M., Adachi, S., Nagahama, Y., Matsuura, S.: Diurnal rhythm of oocyte development and plasma steroid hormone levels in the female red sea, Pagrus major, during the spawning season. Aquaculture, 1988; 73: 357-372.
Kagawa, H., Young, G., Adachi, S., Nagahama, Y.: Estradiol-17β production in amago salmon (Oncorhynchus rhodurus) ovarian follicles: Role of the thecal and granulosa cells. Gen. Comp. Endocrinol., 1982; 47: 440-448.
Kime, D.E., Scott, A.P., Canario A.V.M.: In vitro biosynthesis of steroids, including 11-deoxycortisol and 5α-pregnane- 3β,7α,17,20β-tetrol, by ovaries of the goldfish Carassius auratus during the stage of oocyte final maturation. Gen. Comp. Endocrinol., 1992; 87: 375-384.
Nagahama, Y., Hirose, K., Young, G., Adachi, S., Suzuki, K., Tamaoki, B.: Relative in vitro effectiveness of 17α-20β- dihydroxy-4-pregnen-3-one and other pregnene derivatives on germinal vesicle breakdown on oocytes of ayu (Plecoglossus altivelis), amago salmon (Oncorhynchus rhodurus), rainbow trout (Salmo gairdneri) and goldfish (Carassius auratus). Gen. Comp. Endocrinol., 1983; 51: 15-23.
Asahina, K., Hanyu, I., Aida, K., Nishina, H., Kobayashi, M.: Effects of hyophyseal, placental, hypophysiotropic, and steroid hormones on ovipositor elongation and ovulation in the rose bitterling, Rhodeus ocellatus ocellatus. Gen. Comp. Endocrinol., 1983; 52: 426-437.
Kobayashi, M., Aida, K., Hanyu, I.: Radioimmunoassay for silver carp gonadotropin. Bull. Japan. Soc. Sci. Fish., 1985; 51: 1085- 1091.
Sanngalaj, G.B., Freeman, H.C.: In vitro biosynthesis of 17α,20β dihydroxy-4-pregnen-3-3one by the ovaries, testes, and head kidneys of the atlantic salmon Salmo salar. Gen. Comp. Endocrinol., 1988; 69: 406-415.
Suzuki, K., Tamaoki, B., Hirose, K.: In vitro metabolism of 4- pregnenes in ovaries of a freshwater teleost, the ayu (Plecoglossus altivelis): Production of 17α,20β-dihydroxy-4- pregnen-3-one and its 5β-reduced metabolites, and activation of 3β-and 20β-hydroxysteroid dehydrogenases by treatment with a fish gonadotropin. Gen. Comp. Endocrinol., 1981; 45: 473-481.
Scott, A.P., Sumpter, J.P., Hardiman, P. A.: Hormone changes during ovulation in the rainbow trout (Salmo gairdneri Richardson). Gen. Comp. Endocrinol., 1983; 49: 128-134.