Thiago Marquez FERNANDES, Paulo Roberto ADONA, Samuel GUEMRA, Tiago Henrique Camara DE BEM, Moysés Dos Santos MIRANDA

Oocyte recovery and in vitro embryo production in cows treated with a single dose of follicle-stimulating hormone

Cows were treated with follicle-stimulating hormone (FSH) in a single dose and then subjected to ovum pick-up (OPU) for evaluation of oocyte recovery and in vitro fertilization (IVF). Cows underwent OPU followed by application of FSH (200 mg) on day 2 and new OPU on day 3. This methodology was repeated 3 times sequentially. In FSH-free the cows (control), OPU was performed at intervals of one week. The number of oocytes recovered was higher in the control cows (22.2 ± 1.5) compared to the treated cows (16.9 ± 1.4; P 0.05). There was no difference between the control cows (47.1 ± 4.2%) and the treated cows (45.3 ± 7.7%) with respect to the percentage of embryos (P > 0.05). There were no differences in pregnancy for the blastocysts between the control and the treated cows (50.0 ± 2.1 and 47.3 ± 2.5%; P > 0.05), respectively. In conclusion, the single-dose FSH protocol did not significantly alter the evaluated results of viable oocytes, in vitro production of embryos, or pregnancy percentage. However, there is a possibility of several consecutive OPU/IVF sessions every three days.

Keywords:

Bovine, cryopreservation, ovum pick-up, pregnancy,

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