Morphological diagnosis of Johne’s disease: a case report in cattle
The present case report deals with the morphological (gross and histopathological) and histochemical characterization of
Johne?s disease in a cow. The carcass was presented in a hidebound condition with sunken eyes and with a history of persistent and
nonresponsive diarrhea. Necropsy revealed generalized gelatinization of the visceral and peripheral fat. Thorough gross examination
unveiled major pathological changes in the digestive tract. The gallbladder was distended, with severely thickened walls and blood
mixed with bile. The small intestine was severely congested and the mesenteric lymph nodes were enlarged. The opening of the small
intestine showed marked thickening of the intestinal walls, with a corrugated appearance. Diffuse granulomatous lesions were observed
in the mesenteric lymph nodes. Histopathological examination revealed severe infiltration of intestinal layers and mesenteric lymph
nodes by macrophages, lymphocytes, and epithelioid cells with multiple Langhans giant cells. Ziehl=Neelsen staining demonstrated
multiple acid-fast bacilli in the cytoplasm of macrophages from impression smears.
___
- Al Hajri SM, Alluwaimi AM. ELISA and PCR for evaluation
of subclinical paratuberculosis in the Saudi dairy herds
.
Vet
Microbiol 2007; 121: 384-385.
- Stabel JR. Johne’s disease: a hidden threat
.
J. Dairy Sci 1997; 81:
283-288.
- Stabel JR, Wells SJ, Wagner BA. Relationships between fecal
culture, ELISA, and bulk tank milk test results for Johne’s
disease in US dairy herds
.
J Dairy Sci 2002; 85: 525-531.
- Whittington RJ, Windsor PA. In utero infection of cattle with
Mycobacterium avium
subsp.
paratuberculosis
: a critical review
and meta-analysis
.
Vet J 2009; 179: 60-69.
- Naser SA, Hulten K, Shafran I, Graham DY, El-Zaatari FA.
Specific seroreactivity of Crohn’s disease patients against p35
and p36 antigens of
M. avium
subsp.
paratuberculosis.
Vet
Microbiol 2000; 77: 497-504.
- Whitlock RH, Buergelt C. Preclinical and clinical manifestations
of paratuberculosis (including pathology)
.
Vet Clin North Am
Food Anim Pract 1996; 12: 345-356.
- Mahmoud OM, Haroun EM, Elfaki MG, Abbas B. Pigmented
paratuberculosis granulomata in the liver of sheep
.
Small Rumin
Res 2002; 43: 211- 217.
- Perez V, Garcia Marin JF, Badiola JJ. Description and
classification of different types of lesion associated with natural
paratuberculosis infection in sheep
.
J Comp Pathol 1996; 114:
107-122.
- Smith SL, Wilson PR, Collett MG, Heuer C, West DM, Stevenson
M, Chambers JP. Liver biopsy histopathology for diagnosis of
Johne’s disease in sheep
.
Vet Pathol 2014; 51: 915-918.
- Clarke CJ, Little D. The pathology of ovine paratuberculosis:
gross and histological changes in the intestine and other tissues
.
J Comp Pathol 1996; 114: 419-437.
- Singh AV, Singh SV, Singh PK, Sohal JS, Singh MK. High
prevalence of
Mycobacterium avium
subspecies
paratuberculosis
(‘Indian bison type’) in animal attendants suffering from
gastrointestinal complaints who work with goat herds endemic
for Johne’s disease in India
.
Int J Infect D is 2011; 15: e677-683.